ID   XB32_ORYSJ              Reviewed;         496 AA.
AC   Q6KAE5; A3AC59;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Probable E3 ubiquitin-protein ligase XBOS32;
DE            EC=2.3.2.27;
DE   AltName: Full=Ankyrin repeat domain and RING finger-containing protein XBOS32;
DE   AltName: Full=RING-type E3 ubiquitin transferase XBOS32;
DE   AltName: Full=XB3 protein homolog 2;
GN   Name=XBOS32; OrderedLocusNames=Os02g0791200, LOC_Os02g54860;
GN   ORFNames=OJ1046_F07.26, OsJ_08676;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD19119.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAF10271.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAH00102.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AP003984; BAD19119.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP008208; BAF10271.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM000139; EAZ24898.1; -; Genomic_DNA.
DR   EMBL; AK120632; BAH00102.1; ALT_INIT; mRNA.
DR   RefSeq; XP_015622987.1; XM_015767501.1.
DR   RefSeq; XP_015622989.1; XM_015767503.1.
DR   AlphaFoldDB; Q6KAE5; -.
DR   SMR; Q6KAE5; -.
DR   STRING; 4530.OS02T0791200-01; -.
DR   PaxDb; Q6KAE5; -.
DR   GeneID; 4330973; -.
DR   KEGG; osa:4330973; -.
DR   eggNOG; ENOG502QR1Y; Eukaryota.
DR   HOGENOM; CLU_035461_0_0_1; -.
DR   InParanoid; Q6KAE5; -.
DR   OrthoDB; 539223at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6KAE5; OS.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:UniProt.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.25.40.20; -; 2.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   Pfam; PF12796; Ank_2; 3.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 6.
DR   SMART; SM00184; RING; 1.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 4.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   2: Evidence at transcript level;
KW   ANK repeat; Metal-binding; Reference proteome; Repeat; Transferase;
KW   Ubl conjugation pathway; Zinc; Zinc-finger.
FT   CHAIN           1..496
FT                   /note="Probable E3 ubiquitin-protein ligase XBOS32"
FT                   /id="PRO_0000395746"
FT   REPEAT          50..79
FT                   /note="ANK 1"
FT   REPEAT          83..112
FT                   /note="ANK 2"
FT   REPEAT          117..147
FT                   /note="ANK 3"
FT   REPEAT          180..209
FT                   /note="ANK 4"
FT   REPEAT          223..252
FT                   /note="ANK 5"
FT   ZN_FING         321..368
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ   SEQUENCE   496 AA;  53617 MW;  6DE90A25CDC989DB CRC64;
     MGFLSLVGNS FGCSASGERL VSAARDGDLQ EARALLEYNP RLARYSTFGG RNSPLHYAAA
     QGHHEIVSLL LESGVEINLR NYRGQTALMQ ACQYGHWEVV QTLMLFNANV HRTDYLNGGS
     ALHFAALHGH ARCLRLVLAD YVPSMPNFWN SMKDSLSEEG PSADLDEDGL FKMVNQKADG
     GLTPLHMAAL NGHVECVQLL LDLGASVIEA TIEDGTTIDL IGAGSTPLHY AACGGNAVCC
     QLLIARGASL SAQNASGWTP LMVARSWHRN SLEEILSKEP ESRIRTVPSP YLCLPLMSIM
     SIAREFGWRY LNQSPVCIDP CAVCLEGSCS VAAEGCKHEF CTRCALYLCS TSYTSVSPAG
     AIPCPLCRHP IIAFTALPGT SPIRELPRNS LSLSFCTTCP AVNSDSTPSI ASHLYRTEFQ
     CARMPPMGSS SFRSLSCQRL PAMKLNPSFC MGAMDTNPCL IRCSRFGPSF RRSASQGESS
     RRAWPLTFDP IAATGS