ID   XERA_PYRHO              Reviewed;         285 AA.
AC   O59490;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Tyrosine recombinase XerA {ECO:0000255|HAMAP-Rule:MF_02055};
GN   Name=xerA {ECO:0000255|HAMAP-Rule:MF_02055}; OrderedLocusNames=PH1826;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC       the cutting and rejoining of the recombining DNA molecules.
CC       {ECO:0000255|HAMAP-Rule:MF_02055}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02055}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. XerA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02055}.
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DR   EMBL; BA000001; BAA30945.1; -; Genomic_DNA.
DR   PIR; B71194; B71194.
DR   RefSeq; WP_010885885.1; NC_000961.1.
DR   AlphaFoldDB; O59490; -.
DR   SMR; O59490; -.
DR   STRING; 70601.3258262; -.
DR   EnsemblBacteria; BAA30945; BAA30945; BAA30945.
DR   GeneID; 1442667; -.
DR   KEGG; pho:PH1826; -.
DR   eggNOG; arCOG01241; Archaea.
DR   OMA; HSFASHM; -.
DR   OrthoDB; 57897at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   HAMAP; MF_02055; Recomb_XerA; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   InterPro; IPR033686; XerA.
DR   Pfam; PF02899; Phage_int_SAM_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA integration; DNA recombination; DNA-binding.
FT   CHAIN           1..285
FT                   /note="Tyrosine recombinase XerA"
FT                   /id="PRO_0000095357"
FT   DOMAIN          7..84
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          100..274
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        135
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT   ACT_SITE        160
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT   ACT_SITE        229
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT   ACT_SITE        252
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
FT   ACT_SITE        261
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02055"
SQ   SEQUENCE   285 AA;  32859 MW;  542BF09FEBFCE9F7 CRC64;
     MKEREEIVNS DILEEFATYL ELEGKSKNTI RMYTYYLSKF FEEGYSPTAR DALKFLAKLR
     KSGYSIRSLN LVIQALKSYF KFEGLDSEAE KLKNPKIPKS LPKSLTEDEV KKIVSVADNL
     RDKLILLLLY GAGLRVSELC NLKIEDVNFE KSFLIVRGGK GGKDRVIPIS KTLLFEIERY
     LKTRKDNSPY LFVEKRRNRK DKLSPKTVWM LVKKYGKKVG LNVTPHQLRH SFATHMLERG
     VDIRIIQELL GHANLSTTQI YTKVTTKHLR EAIEKAKLIE TILGG