ID   XERA_SACS2              Reviewed;         291 AA.
AC   Q980D9;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Tyrosine recombinase XerA {ECO:0000255|HAMAP-Rule:MF_02055, ECO:0000305};
GN   Name=xerA {ECO:0000255|HAMAP-Rule:MF_02055, ECO:0000303|PubMed:21113132};
GN   Synonyms=xerC/D {ECO:0000312|EMBL:AAK40704.1};
GN   OrderedLocusNames=SSO0375 {ECO:0000312|EMBL:AAK40704.1};
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   FUNCTION, AND DNA-BINDING.
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=21113132; DOI=10.1038/emboj.2010.301;
RA   Duggin I.G., Dubarry N., Bell S.D.;
RT   "Replication termination and chromosome dimer resolution in the archaeon
RT   Sulfolobus solfataricus.";
RL   EMBO J. 30:145-153(2011).
CC   -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC       the cutting and rejoining of the recombining DNA molecules. Probably
CC       involved in the resolution of chromosome dimers. Binds to the dif site.
CC       {ECO:0000269|PubMed:21113132}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02055}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. XerA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02055}.
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DR   EMBL; AE006641; AAK40704.1; -; Genomic_DNA.
DR   PIR; A99181; A99181.
DR   RefSeq; WP_009990677.1; NC_002754.1.
DR   AlphaFoldDB; Q980D9; -.
DR   SMR; Q980D9; -.
DR   STRING; 273057.SSO0375; -.
DR   EnsemblBacteria; AAK40704; AAK40704; SSO0375.
DR   GeneID; 44129348; -.
DR   KEGG; sso:SSO0375; -.
DR   PATRIC; fig|273057.12.peg.368; -.
DR   eggNOG; arCOG01241; Archaea.
DR   HOGENOM; CLU_027562_9_2_2; -.
DR   InParanoid; Q980D9; -.
DR   OMA; RIEVKHY; -.
DR   PhylomeDB; Q980D9; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   HAMAP; MF_02055; Recomb_XerA; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   InterPro; IPR033686; XerA.
DR   Pfam; PF13495; Phage_int_SAM_4; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA integration; DNA recombination; DNA-binding;
KW   Reference proteome.
FT   CHAIN           1..291
FT                   /note="Tyrosine recombinase XerA"
FT                   /id="PRO_0000435796"
FT   DOMAIN          9..102
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          115..279
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        150
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
FT   ACT_SITE        175
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
FT   ACT_SITE        231
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
FT   ACT_SITE        234
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
FT   ACT_SITE        257
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
FT   ACT_SITE        266
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT                   Rule:MF_02055"
SQ   SEQUENCE   291 AA;  33539 MW;  CDDB9519ED65B129 CRC64;
     MKLDLGSPPE SGDLYNAFIN ALIIAGAGNG TIKLYSTAVR DFLDFINKDP RKVTSEDLNR
     WISSLLNREG KVKGDEVEKK RAKSVTIRYY IIAVRRFLKW INVSVRPPIP KVRRKEVKAL
     DEIQIQKVLN ACKRTKDKLI IRLLLDTGLR ANELLSVLVK DIDLENNMIR VRNTKNGEER
     IVFFTDETKL LLRKYIKGKK AEDKLFDLKY DTLYRKLKRL GKKVGIDLRP HILRHTFATL
     SLKRGINVIT LQKLLGHKDI KTTQIYTHLV LDDLRNEYLK AMSSSSSKTP P