XERC_LACLE
ID XERC_LACLE Reviewed; 295 AA.
AC Q48733;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Tyrosine recombinase XerC {ECO:0000255|HAMAP-Rule:MF_01808};
GN Name=xerC {ECO:0000255|HAMAP-Rule:MF_01808};
OS Lactobacillus leichmannii.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=28039;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 4797 / DSM 20076 / BCRC 10699 / JCM 1148 / NBRC 3073 / NCIMB
RC 7854 / 326 / F59;
RX PubMed=8867465; DOI=10.1007/s002849900042;
RA Becker J., Brendel M.;
RT "Molecular characterization of the xerC gene of Lactobacillus leichmannii
RT encoding a site-specific recombinase and two adjacent heat shock genes.";
RL Curr. Microbiol. 32:232-236(1996).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. The XerC-
CC XerD complex is essential to convert dimers of the bacterial chromosome
CC into monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids.
CC {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD. {ECO:0000255|HAMAP-
CC Rule:MF_01808}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01808}.
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DR EMBL; X84261; CAA59018.1; -; Genomic_DNA.
DR AlphaFoldDB; Q48733; -.
DR SMR; Q48733; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR011931; Recomb_XerC.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR TIGRFAMs; TIGR02224; recomb_XerC; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..295
FT /note="Tyrosine recombinase XerC"
FT /id="PRO_0000095299"
FT DOMAIN 1..84
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 105..289
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 145
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 169
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 241
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 244
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 267
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 276
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
SQ SEQUENCE 295 AA; 33719 MW; EAEA676E187F5042 CRC64;
MTLEEQFLSY LKNERSYSPK TVLAYQKDLA AAKKFWQENG GFPGWDQISR RDLEIYLLAT
GQKLASSTLS RKLSSLKSFY RLLTRRGLVK ADPTVAIQLR RGKKKLPEFF YQDEVGQVIR
SLNDGKPLTV RNRAIVALFY ATGMRLSELT DLKIKQLDLE NGMILVHGKG NKDRYVFFDQ
ESKKYLEEYL QVARPSLLKN EPDTEAVFLN KLGRPISSRG IAKAVQQIFQ KAGLTAGAHP
HELRHSFATA MLNNGADLRS VQELLGHEDL STTQIYTHVS MQHLTAEYRQ HFPRK