XERC_MYCTU
ID XERC_MYCTU Reviewed; 298 AA.
AC P9WF35; L0TB67; P67628; Q10815;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Tyrosine recombinase XerC;
GN Name=xerC; OrderedLocusNames=Rv2894c; ORFNames=MTCY274.25c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. The XerC-
CC XerD complex is essential to convert dimers of the bacterial chromosome
CC into monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45696.1; -; Genomic_DNA.
DR PIR; A70926; A70926.
DR RefSeq; NP_217410.1; NC_000962.3.
DR RefSeq; WP_003414689.1; NZ_NVQJ01000006.1.
DR AlphaFoldDB; P9WF35; -.
DR SMR; P9WF35; -.
DR STRING; 83332.Rv2894c; -.
DR PaxDb; P9WF35; -.
DR DNASU; 887392; -.
DR GeneID; 45426881; -.
DR GeneID; 887392; -.
DR KEGG; mtu:Rv2894c; -.
DR TubercuList; Rv2894c; -.
DR eggNOG; COG4974; Bacteria.
DR OMA; QAFWYLI; -.
DR PhylomeDB; P9WF35; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT CHAIN 1..298
FT /note="Tyrosine recombinase XerC"
FT /id="PRO_0000095306"
FT DOMAIN 1..84
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 105..292
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 149
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 173
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 244
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 247
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 270
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 279
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ SEQUENCE 298 AA; 31980 MW; 640AEE781D523715 CRC64;
MQAILDEFDE YLALQCGRSV HTRRAYLGDL RSLFAFLADR GSSLDALTLS VLRSWLAATA
GAGAARTTLA RRTSAVKAFT AWAVRRGLLA GDPAARLQVP KARRTLPAVL RQDQALRAMA
AAESGAEQGD PLALRDRLIV ELLYATGIRV SELCGLDVDD IDTGHRLVRV LGKGNKQRTV
PFGQPAADAL HAWLVDGRRA LVTAESGHAL LLGARGRRLD VRQARTAVHQ TVAAVDGAPD
MGPHGLRHSA ATHLLEGGAD LRVVQELLGH SSLATTQLYT HVAVARLRAV HERAHPRA
