XERC_PROMI
ID XERC_PROMI Reviewed; 307 AA.
AC O31207;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Tyrosine recombinase XerC {ECO:0000255|HAMAP-Rule:MF_01808};
GN Name=xerC {ECO:0000255|HAMAP-Rule:MF_01808};
OS Proteus mirabilis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Morganellaceae; Proteus.
OX NCBI_TaxID=584;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=UM-240-82;
RX PubMed=9675854; DOI=10.1111/j.1574-6968.1998.tb13071.x;
RA Villion M., Szatmari G.;
RT "Cloning and characterisation of the Proteus mirabilis xerD gene.";
RL FEMS Microbiol. Lett. 164:83-90(1998).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Binds
CC cooperatively to specific DNA consensus sequences that are separated
CC from XerD binding sites by a short central region, forming the
CC heterotetrameric XerC-XerD complex that recombines DNA substrates. The
CC complex is essential to convert dimers of the bacterial chromosome into
CC monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids. In the complex
CC XerC specifically exchanges the top DNA strands. {ECO:0000255|HAMAP-
CC Rule:MF_01808}.
CC -!- ACTIVITY REGULATION: FtsK may regulate the catalytic switch between
CC XerC and XerD in the heterotetrameric complex during the two steps of
CC the recombination process. {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD, in which XerC interacts
CC with XerD via its C-terminal region, XerD interacts with XerC via its
CC C-terminal region and so on. {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01808}.
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DR EMBL; AF033498; AAB87500.1; -; Genomic_DNA.
DR RefSeq; WP_004246325.1; NZ_WURR01000011.1.
DR AlphaFoldDB; O31207; -.
DR SMR; O31207; -.
DR STRING; 584.AOUC001_17880; -.
DR PRIDE; O31207; -.
DR GeneID; 6800511; -.
DR PATRIC; fig|584.106.peg.216; -.
DR OMA; HSFASHM; -.
DR OrthoDB; 745068at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR011931; Recomb_XerC.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR TIGRFAMs; TIGR02224; recomb_XerC; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..307
FT /note="Tyrosine recombinase XerC"
FT /id="PRO_0000095313"
FT DOMAIN 9..95
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 116..296
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 155
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 179
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 248
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 251
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 274
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 283
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
SQ SEQUENCE 307 AA; 34882 MW; F314DB2A374BBCBE CRC64;
MSQIIDVPET LSLAIDSFLS YIEVERRLSP VTVENYQRQL MTIAQMMVAI KINQWSLLES
QHVRMLLAKS HRSGLQPASL ALRFSALRSF LDWQVSQGML AVNPAKGVRT PKSGRHLPKN
MDVDEVSQLM NIDLKDPLSV RDRTMLEVMY GAGLRLSELT NLNINDIDLQ EGEVRVLGKG
SKERKVPLGR KAVEWLQHWF AMRELYSPED TAVFISTKSG KRLSVRSVQK RFELWGVKQG
LSSHVNPHKL RHSFATHLLE SSGDLRAVQE LLGHANLSTT QVYTHLDFQH LAKVYDAAHP
RAKREKS
