XERC_PSEFL
ID XERC_PSEFL Reviewed; 299 AA.
AC Q8VS06; O05324;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Tyrosine recombinase XerC {ECO:0000255|HAMAP-Rule:MF_01808};
GN Name=xerC {ECO:0000255|HAMAP-Rule:MF_01808}; Synonyms=sss;
OS Pseudomonas fluorescens.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=294;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ROOT COLONIZATION.
RC STRAIN=WCS365;
RX PubMed=9618537; DOI=10.1073/pnas.95.12.7051;
RA Dekkers L.C., Phoelich C.C., van der Fits L., Lugtenberg B.J.J.;
RT "A site-specific recombinase is required for competitive root colonization
RT by Pseudomonas fluorescens WCS365.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:7051-7056(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=F113;
RX PubMed=11872710; DOI=10.1128/jb.184.6.1587-1596.2002;
RA Sanchez-Contreras M., Martin M., Villacieros M., O'Gara F., Bonilla I.,
RA Rivilla R.;
RT "Phenotypic selection and phase variation occur during alfalfa root
RT colonization by Pseudomonas fluorescens F113.";
RL J. Bacteriol. 184:1587-1596(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Q8r1-96;
RA Mavrodi O.V., Mavrodi D.V., Weller D.M., Thomashow L.S.;
RT "Role of sss recombinase gene in wheat rhizosphere colonization by
RT Pseudomonas fluorescens Q8r1-96.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. The XerC-
CC XerD complex is essential to convert dimers of the bacterial chromosome
CC into monomers to permit their segregation at cell division. It also
CC contributes to the segregational stability of plasmids.
CC {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC molecules of XerC and two molecules of XerD. {ECO:0000255|HAMAP-
CC Rule:MF_01808}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01808}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01808}.
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DR EMBL; Y12268; CAA72946.1; -; Genomic_DNA.
DR EMBL; AF416734; AAL73390.1; -; Genomic_DNA.
DR EMBL; AY172655; AAO17715.1; -; Genomic_DNA.
DR PIR; T10461; T10461.
DR RefSeq; WP_003206809.1; NZ_QQAQ01000005.1.
DR AlphaFoldDB; Q8VS06; -.
DR SMR; Q8VS06; -.
DR STRING; 690597.JH730915_gene4357; -.
DR GeneID; 57262434; -.
DR PATRIC; fig|294.126.peg.1037; -.
DR eggNOG; COG4973; Bacteria.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR011931; Recomb_XerC.
DR InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR TIGRFAMs; TIGR02224; recomb_XerC; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..299
FT /note="Tyrosine recombinase XerC"
FT /id="PRO_0000095315"
FT DOMAIN 1..85
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 106..285
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 146
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 170
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 237
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 240
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 263
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT ACT_SITE 272
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01808"
FT CONFLICT 34
FT /note="L -> V (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="D -> N (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
FT CONFLICT 131
FT /note="R -> Q (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="P -> A (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
FT CONFLICT 211
FT /note="R -> K (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="Missing (in Ref. 2; AAL73390)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 299 AA; 33772 MW; 842FFD0203AD8984 CRC64;
MERQLDAYCE HLRSERQVSP HTLSAYRRDL EKVLGWCQKQ NIGSWAALDI QRLRSLIARL
HQQGQSSRSL ARLLSAVRGL YHYLNREGLC DHDPATGLAP PKGERRLPKT LDTDRALQLL
EGAVEDDFLA RRDQAILELF YSSGLRLSEL TGLNLDQLDL ADGMVQVLGK GSKTRLLPVG
RKAREALEQW LPLRALTNPA DDAVFVSQQG RRLGPRAIQL RVKAAGEREL GQNLHPHMLR
HSFASHLLES SQDLRAVQEL LGHSDIKTTQ IYTHLDFQHL ATVYDSAHPR AKRIKGDES
