ID   XERC_SERMA              Reviewed;         303 AA.
AC   O31087;
DT   22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Tyrosine recombinase XerC;
GN   Name=xerC;
OS   Serratia marcescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], OVEREXPRESSION OF NUCA, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=SM6;
RX   PubMed=9555916; DOI=10.1128/jb.180.8.2262-2264.1998;
RA   Guynn L.J., Dai W., Benedik M.J.;
RT   "Nuclease overexpression mutants of Serratia marcescens.";
RL   J. Bacteriol. 180:2262-2264(1998).
CC   -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC       the cutting and rejoining of the recombining DNA molecules. Binds
CC       cooperatively to specific DNA consensus sequences that are separated
CC       from XerD binding sites by a short central region, forming the
CC       heterotetrameric XerC-XerD complex that recombines DNA substrates. The
CC       complex is essential to convert dimers of the bacterial chromosome into
CC       monomers to permit their segregation at cell division. It also
CC       contributes to the segregational stability of plasmids. In the complex
CC       XerC specifically exchanges the top DNA strands (By similarity).
CC       {ECO:0000250}.
CC   -!- ACTIVITY REGULATION: FtsK may regulate the catalytic switch between
CC       XerC and XerD in the heterotetrameric complex during the two steps of
CC       the recombination process. {ECO:0000250}.
CC   -!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
CC       molecules of XerC and two molecules of XerD, in which XerC interacts
CC       with XerD via its C-terminal region, XerD interacts with XerC via its
CC       C-terminal region and so on. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Defects result in partial SOS induction, inducing
CC       some overexpression of the nuclease NucA protein.
CC       {ECO:0000269|PubMed:9555916}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. XerC subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF028736; AAC46276.1; -; Genomic_DNA.
DR   RefSeq; WP_033645020.1; NZ_WUUW01000013.1.
DR   AlphaFoldDB; O31087; -.
DR   SMR; O31087; -.
DR   STRING; 273526.SMDB11_4288; -.
DR   GeneID; 64310532; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006313; P:transposition, DNA-mediated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   HAMAP; MF_01808; Recomb_XerC_XerD; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   InterPro; IPR011931; Recomb_XerC.
DR   InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
DR   Pfam; PF02899; Phage_int_SAM_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   TIGRFAMs; TIGR02224; recomb_XerC; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW   DNA integration; DNA recombination; DNA-binding.
FT   CHAIN           1..303
FT                   /note="Tyrosine recombinase XerC"
FT                   /id="PRO_0000095326"
FT   DOMAIN          6..92
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          113..292
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        152
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        176
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        244
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        247
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        270
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        279
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   303 AA;  34115 MW;  093C508C55A46456 CRC64;
     MTPIAPSLQQ PVDAFLRYLK VERQLSPLTQ LSYSRQLTAL MRLAQEIGVT DWTTLDAARV
     RMLAARSKRA GLQSASLALR LSSLRSFLDW LVSQGVLHAN PAKGIRTPRS GRHLPKNIDV
     DEMNQLLEID LNDPLAVRDR AMLEVMYGAG LRLSELVGLD CRHVDMAAGE VWVMGKGSKE
     RKLPIGRTAV TWLEHWLAMR DLFGPQDDAM FLSNQGRRIS TRNVQKRFAE WGVKQGVNSH
     IHPHKLRHSF ATHMLESSGD LRAVQELLGH ANLTTTQIYT HLDFQHLANV YDAAHPRAKR
     GKS