CAP10_ARATH
ID CAP10_ARATH Reviewed; 544 AA.
AC Q9LHS0;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Putative clathrin assembly protein At5g35200;
GN OrderedLocusNames=At5g35200; ORFNames=K3D20.4, T25C13.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBCELLULAR LOCATION: Membrane, clathrin-coated pit {ECO:0000250}.
CC Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000250}. Note=Colocalized with clathrin in the Golgi
CC area. {ECO:0000250}.
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DR EMBL; AP002031; BAA98205.1; -; Genomic_DNA.
DR EMBL; AF080121; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93944.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68678.1; -; Genomic_DNA.
DR EMBL; AY081284; AAL91173.1; -; mRNA.
DR EMBL; BT000375; AAN15694.1; -; mRNA.
DR RefSeq; NP_001330406.1; NM_001344100.1.
DR RefSeq; NP_198370.1; NM_122911.4.
DR AlphaFoldDB; Q9LHS0; -.
DR SMR; Q9LHS0; -.
DR STRING; 3702.AT5G35200.1; -.
DR iPTMnet; Q9LHS0; -.
DR PaxDb; Q9LHS0; -.
DR PRIDE; Q9LHS0; -.
DR ProteomicsDB; 239148; -.
DR EnsemblPlants; AT5G35200.1; AT5G35200.1; AT5G35200.
DR EnsemblPlants; AT5G35200.2; AT5G35200.2; AT5G35200.
DR GeneID; 833474; -.
DR Gramene; AT5G35200.1; AT5G35200.1; AT5G35200.
DR Gramene; AT5G35200.2; AT5G35200.2; AT5G35200.
DR KEGG; ath:AT5G35200; -.
DR Araport; AT5G35200; -.
DR TAIR; locus:2182432; AT5G35200.
DR eggNOG; KOG0251; Eukaryota.
DR HOGENOM; CLU_014098_2_1_1; -.
DR InParanoid; Q9LHS0; -.
DR OMA; QMMMMGP; -.
DR OrthoDB; 553740at2759; -.
DR PhylomeDB; Q9LHS0; -.
DR PRO; PR:Q9LHS0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LHS0; baseline and differential.
DR Genevisible; Q9LHS0; AT.
DR GO; GO:0005905; C:clathrin-coated pit; IBA:GO_Central.
DR GO; GO:0030136; C:clathrin-coated vesicle; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005545; F:1-phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0032050; F:clathrin heavy chain binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0048268; P:clathrin coat assembly; IEA:InterPro.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IBA:GO_Central.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR Gene3D; 1.20.58.150; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR011417; ANTH_dom.
DR InterPro; IPR014712; ANTH_dom_sf.
DR InterPro; IPR045192; AP180-like.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR PANTHER; PTHR22951; PTHR22951; 1.
DR Pfam; PF07651; ANTH; 1.
DR SMART; SM00273; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
PE 1: Evidence at protein level;
KW Coated pit; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..544
FT /note="Putative clathrin assembly protein At5g35200"
FT /id="PRO_0000187076"
FT DOMAIN 28..166
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT REGION 338..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 342
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 544 AA; 61179 MW; 344A875061D62323 CRC64;
MSGGGGSQSS LRRYLGAIKD TTTVSLAKVN SDYKELDIAI VKATNHVERP SKERYIRAIF
MAISATRPRA DVAYCIHALA RRLSRTHNWA VALKTLIVIH RALREVDQTF HEEVINYSRS
RSHMLNMSHF KDDSGPNAWA YSAWVRFYAL FLEERLECFR VLKYDVEVDP PRTKDLDTPD
LLEQLPALQE LLFRVLDCQP EGAAVQNHII QLALSMVISE STKIYQALTD GIDNLVDKFF
DMQRNDAVKA LDMYRRAVKQ AGRLSEFFEV CKSVNVGRGE RFIKIEQPPT SFLQAMEEYV
KEAPLAAGVK KEQVVEKLTA PKEILAIEYE IPPKVVEEKP ASPEPVKAEA EKPVEKQPDL
LSMDDPAPMV SELEEKNALA LAIVPVSVEQ PHSTTDFTNG NSTGWELALV TAPSSNEGAA
ADSKLAGGLD KLTLDSLYED AIRVSQQQNR SYNPWEQNPV HNGHMMHQPF YASNGVAAPQ
PFQMANQNHQ TFGYQHQNAG MMMGPVQQPY QQQQQNMNNP FGNPFVSNGN PQQPQGYNPY
PRYM
