XERH_CAMJE
ID XERH_CAMJE Reviewed; 354 AA.
AC Q0PA27;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Tyrosine recombinase XerH {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000305};
GN Name=xerH {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000303|PubMed:23861023};
GN Synonyms=xerD {ECO:0000312|EMBL:CAL34991.1};
GN OrderedLocusNames=Cj0863c {ECO:0000312|EMBL:CAL34991.1};
OS Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS 11168).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Campylobacteraceae; Campylobacter.
OX NCBI_TaxID=192222;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700819 / NCTC 11168;
RX PubMed=10688204; DOI=10.1038/35001088;
RA Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA Barrell B.G.;
RT "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT reveals hypervariable sequences.";
RL Nature 403:665-668(2000).
RN [2]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, AND MUTAGENESIS OF TYR-333.
RC STRAIN=RM1221;
RX PubMed=23861023; DOI=10.1007/s00438-013-0765-5;
RA Leroux M., Rezoug Z., Szatmari G.;
RT "The Xer/dif site-specific recombination system of Campylobacter jejuni.";
RL Mol. Genet. Genomics 288:495-502(2013).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Binds to
CC the complete atypical dif motif (difH) site and to both halves
CC separately. {ECO:0000269|PubMed:23861023}.
CC -!- ACTIVITY REGULATION: FtsK is required for efficient recombination.
CC {ECO:0000269|PubMed:23861023}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02054,
CC ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000305}.
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DR EMBL; AL111168; CAL34991.1; -; Genomic_DNA.
DR PIR; G81359; G81359.
DR RefSeq; WP_002852607.1; NC_002163.1.
DR RefSeq; YP_002344270.1; NC_002163.1.
DR AlphaFoldDB; Q0PA27; -.
DR SMR; Q0PA27; -.
DR IntAct; Q0PA27; 12.
DR STRING; 192222.Cj0863c; -.
DR PaxDb; Q0PA27; -.
DR PRIDE; Q0PA27; -.
DR EnsemblBacteria; CAL34991; CAL34991; Cj0863c.
DR GeneID; 905165; -.
DR KEGG; cje:Cj0863c; -.
DR PATRIC; fig|192222.6.peg.851; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_027562_9_6_7; -.
DR OMA; EKNGAHM; -.
DR Proteomes; UP000000799; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_02054; Recomb_XerH; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR041308; Xer_N.
DR InterPro; IPR033683; XerH.
DR Pfam; PF18644; Phage_int_SAM_6; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; DNA integration; DNA recombination;
KW DNA-binding; Reference proteome.
FT CHAIN 1..354
FT /note="Tyrosine recombinase XerH"
FT /id="PRO_0000435725"
FT DOMAIN 48..134
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 163..346
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 231
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 298
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 301
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 324
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 333
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT MUTAGEN 333
FT /note="Y->F: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23861023"
SQ SEQUENCE 354 AA; 40611 MW; 6660C22931FBCCCD CRC64;
MKYPLDCEEN FEKSFLFWLA KYVKFKLNSL SNKELKNPQA LAEVNFALTK GVKNIDELDA
LAKKARNAGL SGVNTYFNPL KKVFEYLNFY KLYSLKQIDE ELIVEVLASI TGALSDASKK
NYRIAVINFF DFLDKQNEED EKAHIFDINL KNWAGIAGSK GVKLPEFMSK EELKKFLDAI
ENADFKNNTI RNKLIIKIII FTGIRVSEAI NIKMGDISEE NDLYIIRIRA KGNKYRVVMI
KKELIYDLLK NVSINYISKD ALLFVNKKGT PLTQSYVSRI VEQLLFRAGI RKQKNGAHML
RHTFATLLYK KQKDLVLVQE ALGHASLNTS RIYTHFDNDK LKLAAQVAKE LSDS
