XERH_HELPY
ID XERH_HELPY Reviewed; 362 AA.
AC O25386;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Tyrosine recombinase XerH {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000305};
GN Name=xerH {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000303|PubMed:19727445};
GN OrderedLocusNames=HP_0675 {ECO:0000312|EMBL:AAD07734.1};
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP NOMENCLATURE.
RX PubMed=19727445; DOI=10.1371/journal.pone.0006531;
RA Carnoy C., Roten C.A.;
RT "The dif/Xer recombination systems in proteobacteria.";
RL PLoS ONE 4:E6531-E6531(2009).
RN [3]
RP FUNCTION AS A RECOMBINASE, ACTIVITY REGULATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=22511919; DOI=10.1371/journal.pone.0033310;
RA Debowski A.W., Carnoy C., Verbrugghe P., Nilsson H.O., Gauntlett J.C.,
RA Fulurija A., Camilleri T., Berg D.E., Marshall B.J., Benghezal M.;
RT "Xer recombinase and genome integrity in Helicobacter pylori, a pathogen
RT without topoisomerase IV.";
RL PLoS ONE 7:E33310-E33310(2012).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Involved in
CC chromosome segregation. May contribute to chromosome decatenation.
CC {ECO:0000269|PubMed:22511919}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000269|PubMed:22511919}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02054,
CC ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion blocks difH recombination. Deletion
CC mutant shows impaired chromosome segregation, slight growth defect, UV
CC and ciprofloxacin susceptibility, resistance to oxidative stress and
CC inability to colonize mice. {ECO:0000269|PubMed:22511919}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerH subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_02054, ECO:0000305}.
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DR EMBL; AE000511; AAD07734.1; -; Genomic_DNA.
DR PIR; C64604; C64604.
DR RefSeq; NP_207469.1; NC_000915.1.
DR RefSeq; WP_000682431.1; NC_018939.1.
DR PDB; 5JJV; X-ray; 2.40 A; A/B=1-362.
DR PDB; 5JK0; X-ray; 2.10 A; A/B/C/D=1-362.
DR PDBsum; 5JJV; -.
DR PDBsum; 5JK0; -.
DR AlphaFoldDB; O25386; -.
DR SMR; O25386; -.
DR STRING; 85962.C694_03485; -.
DR PaxDb; O25386; -.
DR EnsemblBacteria; AAD07734; AAD07734; HP_0675.
DR KEGG; hpy:HP_0675; -.
DR PATRIC; fig|85962.47.peg.724; -.
DR eggNOG; COG4974; Bacteria.
DR OMA; EKNGAHM; -.
DR PhylomeDB; O25386; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_02054; Recomb_XerH; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR041308; Xer_N.
DR InterPro; IPR033683; XerH.
DR Pfam; PF18644; Phage_int_SAM_6; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT CHAIN 1..362
FT /note="Tyrosine recombinase XerH"
FT /id="PRO_0000435726"
FT DOMAIN 43..140
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 170..357
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 239
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 309
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 312
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 335
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 344
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT HELIX 11..29
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 38..49
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 54..66
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 70..72
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 73..87
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 106..116
FT /evidence="ECO:0007829|PDB:5JK0"
FT TURN 117..119
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 122..142
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 177..189
FT /evidence="ECO:0007829|PDB:5JK0"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 197..210
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 214..218
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:5JK0"
FT STRAND 225..228
FT /evidence="ECO:0007829|PDB:5JK0"
FT STRAND 231..237
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 239..241
FT /evidence="ECO:0007829|PDB:5JK0"
FT STRAND 243..249
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 254..264
FT /evidence="ECO:0007829|PDB:5JK0"
FT STRAND 273..275
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 285..298
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 308..323
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 326..333
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 338..341
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 342..344
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 349..351
FT /evidence="ECO:0007829|PDB:5JK0"
FT HELIX 352..356
FT /evidence="ECO:0007829|PDB:5JK0"
FT TURN 357..360
FT /evidence="ECO:0007829|PDB:5JK0"
SQ SEQUENCE 362 AA; 41851 MW; C0C3ED465C653C6D CRC64;
MKHPLEELKD PTENLLLWIG RFLRYKCTSL SNSQVKDQNK VFECLNELNQ ACSSSQLEKV
CKKARNAGLL GINTYALPLL KFHEYFSKAR LITERLAFNS LKNIDEVMLA EFLSVYTGGL
SLATKKNYRI ALLGLFSYID KQNQDENEKS YIYNITLKNI SGVNQSAGNK LPTHLNNEEL
EKFLESIDKI EMSAKVRARN RLLIKIIVFT GMRSNEALQL KIKDFTLENG CYTILIKGKG
DKYRAVMLKA FHIESLLKEW LIERELYPVK NDLLFCNQKG SALTQAYLYK QVERIINFAG
LRREKNGAHM LRHSFATLLY QKRHDLILVQ EALGHASLNT SRIYTHFDKQ RLEEAASIWE
EN
