XERS_STRE4
ID XERS_STRE4 Reviewed; 356 AA.
AC C0MBC3;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816}; OrderedLocusNames=SEQ_1186;
OS Streptococcus equi subsp. equi (strain 4047).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=553482;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4047;
RX PubMed=19325880; DOI=10.1371/journal.ppat.1000346;
RA Holden M.T.G., Heather Z., Paillot R., Steward K.F., Webb K., Ainslie F.,
RA Jourdan T., Bason N.C., Holroyd N.E., Mungall K., Quail M.A., Sanders M.,
RA Simmonds M., Willey D., Brooks K., Aanensen D.M., Spratt B.G., Jolley K.A.,
RA Maiden M.C.J., Kehoe M., Chanter N., Bentley S.D., Robinson C.,
RA Maskell D.J., Parkhill J., Waller A.S.;
RT "Genomic evidence for the evolution of Streptococcus equi: host
RT restriction, increased virulence, and genetic exchange with human
RT pathogens.";
RL PLoS Pathog. 5:E1000346-E1000346(2009).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Essential
CC to convert dimers of the bacterial chromosome into monomers to permit
CC their segregation at cell division. {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
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DR EMBL; FM204883; CAW93891.1; -; Genomic_DNA.
DR RefSeq; WP_012679572.1; NC_012471.1.
DR AlphaFoldDB; C0MBC3; -.
DR SMR; C0MBC3; -.
DR EnsemblBacteria; CAW93891; CAW93891; SEQ_1186.
DR KEGG; seu:SEQ_1186; -.
DR HOGENOM; CLU_027562_9_6_9; -.
DR OMA; EKNGAHM; -.
DR OrthoDB; 745068at2; -.
DR Proteomes; UP000001365; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01816; Recomb_XerS; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023670; Recomb_XerS.
DR Pfam; PF13495; Phage_int_SAM_4; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..356
FT /note="Tyrosine recombinase XerS"
FT /id="PRO_1000187911"
FT DOMAIN 16..121
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 169..354
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 210
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 234
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 306
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 309
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 332
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 341
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
SQ SEQUENCE 356 AA; 41429 MW; C1D7CB3740773ECA CRC64;
MKRDLLLTKI EEYKNIMPWY VLDYYQSKLS VPYSFTTLYE YLKEYKRFFE WLIDSDLSKA
ARIADVDLTT LEHLSKKDME AFILYLRERP SLNIYSTKKG VSQTTINRTL SALSSLYKYL
TEEVENEHGE PYFYRNVMKK VATKKKRETL AARAENIKQK LFLGDETMAF LDYVDKEYEY
KLSNRAKASF RKNKERDLAI IALLLASGIR LSEAVNLDLK DVNLNMMLVE VTRKGGKRDS
VNVAAFAKPH LEAYLSVRKD RYQAEKQDVA FFLTAYRGLP NRIDASSIEK MVGKYSEGFK
IRVTPHKLRH TLATRLYDTT KSQVLVSHQL GHASTQVTDL YTHIVNDEQK NALDKL