ID   XERS_STRP4              Reviewed;         356 AA.
AC   B5E4Q4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   23-FEB-2022, entry version 70.
DE   RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN   Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816}; OrderedLocusNames=SPG_1060;
OS   Streptococcus pneumoniae serotype 19F (strain G54).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=512566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G54;
RX   PubMed=11442348; DOI=10.1089/10766290152044995;
RA   Dopazo J., Mendoza A., Herrero J., Caldara F., Humbert Y., Friedli L.,
RA   Guerrier M., Grand-Schenk E., Gandin C., de Francesco M., Polissi A.,
RA   Buell G., Feger G., Garcia E., Peitsch M., Garcia-Bustos J.F.;
RT   "Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F
RT   clinical isolate.";
RL   Microb. Drug Resist. 7:99-125(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G54;
RA   Mulas L., Trappetti C., Hakenbeck R., Iannelli F., Pozzi G., Davidsen T.M.,
RA   Tettelin H., Oggioni M.;
RT   "Pneumococcal beta glucoside metabolism investigated by whole genome
RT   comparison.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC       the cutting and rejoining of the recombining DNA molecules. Essential
CC       to convert dimers of the bacterial chromosome into monomers to permit
CC       their segregation at cell division. {ECO:0000255|HAMAP-Rule:MF_01816}.
CC   -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC       {ECO:0000255|HAMAP-Rule:MF_01816}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01816}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001015; ACF56147.1; -; Genomic_DNA.
DR   RefSeq; WP_000817887.1; NC_011072.1.
DR   KEGG; spx:SPG_1060; -.
DR   HOGENOM; CLU_027562_9_6_9; -.
DR   OMA; EKNGAHM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   HAMAP; MF_01816; Recomb_XerS; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR023670; Recomb_XerS.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW   DNA integration; DNA recombination; DNA-binding.
FT   CHAIN           1..356
FT                   /note="Tyrosine recombinase XerS"
FT                   /id="PRO_1000187913"
FT   DOMAIN          16..121
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          169..354
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        210
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT   ACT_SITE        234
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT   ACT_SITE        306
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT   ACT_SITE        309
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT   ACT_SITE        332
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT   ACT_SITE        341
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
SQ   SEQUENCE   356 AA;  41169 MW;  6FFC67FA1F650550 CRC64;
     MKREILLERI DKLKQLMPWY VLEYYQSKLA VPYSFTTLYE YLKEYDRFFS WVLESGISNA
     DKISDIPLSV LENMSKKDME SFILYXRERP LLNANTTKQG VSQTTINRTL SALSSLYKYL
     TEEVENDQGE PYFYRNVMKK VSTKKKKETL AARAENIKQK LFLGDETEGF LTYIDQEHPQ
     QLSNRALSSF NKNKERDLAI IALLLASGVR LSEAVNLDLR DLNLKMMVID VTRKGGKRDS
     VNVAAFAKPY LENYLAIRNQ RYKTEKTDTA LFLTLYRGVP NRIDASSVEK MVAKYSEDFK
     VRVTPHKLRH TLATRLYDAT KSQVLVSHQL GHASTQVTDL YTHIVNDEQK NALDSL