XERS_STRPF
ID XERS_STRPF Reviewed; 356 AA.
AC Q1J6H1;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN OrderedLocusNames=MGAS10750_Spy1062;
OS Streptococcus pyogenes serotype M4 (strain MGAS10750).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=370554;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGAS10750;
RX PubMed=16636287; DOI=10.1073/pnas.0510279103;
RA Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R.,
RA Musser J.M.;
RT "Molecular genetic anatomy of inter- and intraserotype variation in the
RT human bacterial pathogen group A Streptococcus.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Essential
CC to convert dimers of the bacterial chromosome into monomers to permit
CC their segregation at cell division. {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABF38012.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000262; ABF38012.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_002984598.1; NC_008024.1.
DR AlphaFoldDB; Q1J6H1; -.
DR SMR; Q1J6H1; -.
DR EnsemblBacteria; ABF38012; ABF38012; MGAS10750_Spy1062.
DR KEGG; spi:MGAS10750_Spy1062; -.
DR HOGENOM; CLU_027562_9_6_9; -.
DR Proteomes; UP000002434; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01816; Recomb_XerS; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023670; Recomb_XerS.
DR Pfam; PF13495; Phage_int_SAM_4; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..356
FT /note="Tyrosine recombinase XerS"
FT /id="PRO_0000372675"
FT DOMAIN 16..121
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 169..354
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 210
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 234
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 306
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 309
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 332
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 341
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
SQ SEQUENCE 356 AA; 41472 MW; 08B1E5FBC667864B CRC64;
MRRELLLEKI ETYKAIMPWY VLDYYQSKLA VPYSFTTLYE YLKEYKRFFD WLMDADLTQA
PKIADIDLST LEHLTKKDLE AFVLYLRERP SLNTYSTKEG LSQTTINRTL SALSSLYKYL
TEEVENDQGE PYFYRNVMKK VSTKKKKETL ASRAENIKQK LFLGDETLAF LDYVDKEYEQ
KLSNRAKSSF RKNKERDLAI IALLLASGVR LSEAVNLDLK DVNLNMMIIE VIRKGGKRDS
VNVAGFAKGY LESYLAVRQR RYKAEKQDLA FFLTEYRGVP NRMDASSIEK MVGKYSEDFK
IRVTPHKLRH TLATRLYDAT KSQVLVSHQL GHSSTQVTDL YTHIVNDEQK NALDNL