XERS_STRPN
ID XERS_STRPN Reviewed; 356 AA.
AC Q97QP2;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816}; OrderedLocusNames=SP_1159;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Essential
CC to convert dimers of the bacterial chromosome into monomers to permit
CC their segregation at cell division. {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- INTERACTION:
CC Q97QP2; P63544: apt; NbExp=2; IntAct=EBI-2207218, EBI-2207316;
CC Q97QP2; Q97QS2: eno; NbExp=2; IntAct=EBI-2207218, EBI-2207206;
CC Q97QP2; Q97SE5: gatC; NbExp=2; IntAct=EBI-2207218, EBI-2207053;
CC Q97QP2; Q97NV3: groES; NbExp=2; IntAct=EBI-2207218, EBI-2206949;
CC Q97QP2; Q97S73: grpE; NbExp=2; IntAct=EBI-2207218, EBI-2207065;
CC Q97QP2; P0A4T1: malR; NbExp=2; IntAct=EBI-2207218, EBI-2207435;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
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DR EMBL; AE005672; AAK75268.1; -; Genomic_DNA.
DR PIR; C95134; C95134.
DR RefSeq; WP_000817881.1; NZ_AKVY01000001.1.
DR AlphaFoldDB; Q97QP2; -.
DR SMR; Q97QP2; -.
DR IntAct; Q97QP2; 6.
DR STRING; 170187.SP_1159; -.
DR EnsemblBacteria; AAK75268; AAK75268; SP_1159.
DR KEGG; spn:SP_1159; -.
DR eggNOG; COG4974; Bacteria.
DR OMA; EKNGAHM; -.
DR PhylomeDB; Q97QP2; -.
DR BioCyc; SPNE170187:G1FZB-1179-MON; -.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01816; Recomb_XerS; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023670; Recomb_XerS.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..356
FT /note="Tyrosine recombinase XerS"
FT /id="PRO_0000095363"
FT DOMAIN 16..121
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 169..354
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 210
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 234
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 306
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 309
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 332
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 341
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
SQ SEQUENCE 356 AA; 41190 MW; 1CFC76488B650555 CRC64;
MKREILLERI DKLKQLMPWY VLEYYQSKLA VPYSFTTLYE YLKEYDRFFS WVLESGISNA
DKISDIPLSV LENMSKKDME SFILYLRERP LLNANTTKQG VSQTTINRTL SALSSLYKYL
TEEVENDQGE PYFYRNVMKK VSTKKKKETL AARAENIKQK LFLGDETEGF LTYIDQEHPQ
QLSNRALSSF NKNKERDLAI IALLLASGVR LSEAVNLDLR DLNLKMMVID VTRKGCKRDS
VNVAAFAKPY LENYLAIRNQ RYKTEKTDTA LFLTLYRGVP NRIDASSVEK MVAKYSEDFK
VRVTPHKLRH TLATRLYDAT KSQVLVSHQL GHASTQVTDL YTHIVSDEQK NALDSL