XERS_STRPS
ID XERS_STRPS Reviewed; 356 AA.
AC B2IPW6;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816};
GN Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816}; OrderedLocusNames=SPCG_1138;
OS Streptococcus pneumoniae (strain CGSP14).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=516950;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGSP14;
RX PubMed=19361343; DOI=10.1186/1471-2164-10-158;
RA Ding F., Tang P., Hsu M.-H., Cui P., Hu S., Yu J., Chiu C.-H.;
RT "Genome evolution driven by host adaptations results in a more virulent and
RT antimicrobial-resistant Streptococcus pneumoniae serotype 14.";
RL BMC Genomics 10:158-158(2009).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Essential
CC to convert dimers of the bacterial chromosome into monomers to permit
CC their segregation at cell division. {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01816}.
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DR EMBL; CP001033; ACB90390.1; -; Genomic_DNA.
DR RefSeq; WP_000817882.1; NC_010582.1.
DR AlphaFoldDB; B2IPW6; -.
DR SMR; B2IPW6; -.
DR EnsemblBacteria; ACB90390; ACB90390; SPCG_1138.
DR GeneID; 60233685; -.
DR GeneID; 66806283; -.
DR KEGG; spw:SPCG_1138; -.
DR HOGENOM; CLU_027562_9_6_9; -.
DR OMA; EKNGAHM; -.
DR Proteomes; UP000001682; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01816; Recomb_XerS; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023670; Recomb_XerS.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding.
FT CHAIN 1..356
FT /note="Tyrosine recombinase XerS"
FT /id="PRO_0000372669"
FT DOMAIN 16..121
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 169..354
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 210
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 234
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 306
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 309
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 332
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
FT ACT_SITE 341
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01816"
SQ SEQUENCE 356 AA; 41171 MW; 1E3C68618B650155 CRC64;
MKREILLERI DKLKQLMPWY VLEYYQSKLA VPYSFTTLYE YLKEYDRFFS WVLESGISNA
DKISDIPLSV LENMSKKDME SFILYLRERP LLNANTTKQG VSQTTINRTL SALSSLYKYL
TEEVENDQGE PYFYRNVMKK VSTKKKKETL AARAENIKQK LFLGDETEGF LTYIDQEHPQ
QLSNRALSSF NKNKERDLAI IALLLASGVR LSEAVNLDLR DLNLKMMVID VTRKGGKRDS
VNVAAFAKPY LENYLAIRNQ RYKTEKTDTA LFLTLYRGVP NRIDASSVEK MVAKYSEDFK
VRVTPHKLRH TLATRLYDAT KSQVLVSHQL GHASTQVTDL YTHIVNDEQK NALDSL