XERS_STRR6
ID XERS_STRR6 Reviewed; 356 AA.
AC Q7ZAK7;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Tyrosine recombinase XerS {ECO:0000255|HAMAP-Rule:MF_01816, ECO:0000305};
GN Name=xerS {ECO:0000255|HAMAP-Rule:MF_01816, ECO:0000303|PubMed:17630835};
GN OrderedLocusNames=spr1046;
OS Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=171101;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL J. Bacteriol. 183:5709-5717(2001).
RN [2]
RP FUNCTION AS A RECOMBINASE, AND ACTIVITY REGULATION.
RC STRAIN=ATCC BAA-255 / R6;
RX PubMed=17630835; DOI=10.1371/journal.pgen.0030117;
RA Le Bourgeois P., Bugarel M., Campo N., Daveran-Mingot M.-L., Labonte J.,
RA Lanfranchi D., Lautier T., Pages C., Ritzenthaler P.;
RT "The unconventional Xer recombination machinery of
RT Streptococci/Lactococci.";
RL PLoS Genet. 3:E117-E117(2007).
RN [3]
RP FUNCTION.
RX PubMed=25569614; DOI=10.1371/journal.pgen.1004934;
RA Johnston C., Mortier-Barriere I., Granadel C., Polard P., Martin B.,
RA Claverys J.P.;
RT "RecFOR is not required for pneumococcal transformation but together with
RT XerS for resolution of chromosome dimers frequently formed in the
RT process.";
RL PLoS Genet. 11:E1004934-E1004934(2015).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules. Essential
CC to convert dimers of the bacterial chromosome into monomers to permit
CC their segregation at cell division (PubMed:17630835). Required for
CC resolution of chromosome dimers frequently formed by transformation
CC (PubMed:25569614). {ECO:0000269|PubMed:17630835,
CC ECO:0000269|PubMed:25569614}.
CC -!- ACTIVITY REGULATION: FtsK is required for recombination.
CC {ECO:0000255|HAMAP-Rule:MF_01816, ECO:0000269|PubMed:17630835}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01816,
CC ECO:0000305}.
CC -!- MISCELLANEOUS: In contrast to the XerC-XerD complex present in non-
CC streptococcaceae bacteria, XerS acts as a single recombinase required
CC to recombine difSL recombination site. {ECO:0000305|PubMed:17630835}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerS subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01816, ECO:0000305}.
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DR EMBL; AE007317; AAK99850.1; -; Genomic_DNA.
DR PIR; F98002; F98002.
DR RefSeq; NP_358640.1; NC_003098.1.
DR RefSeq; WP_000817882.1; NC_003098.1.
DR AlphaFoldDB; Q7ZAK7; -.
DR SMR; Q7ZAK7; -.
DR STRING; 171101.spr1046; -.
DR EnsemblBacteria; AAK99850; AAK99850; spr1046.
DR GeneID; 60233685; -.
DR GeneID; 66806283; -.
DR KEGG; spr:spr1046; -.
DR PATRIC; fig|171101.6.peg.1137; -.
DR eggNOG; COG4974; Bacteria.
DR HOGENOM; CLU_027562_9_6_9; -.
DR OMA; EKNGAHM; -.
DR Proteomes; UP000000586; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; -; 1.
DR HAMAP; MF_01816; Recomb_XerS; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR023670; Recomb_XerS.
DR Pfam; PF02899; Phage_int_SAM_1; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT CHAIN 1..356
FT /note="Tyrosine recombinase XerS"
FT /id="PRO_0000095364"
FT DOMAIN 16..121
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 169..354
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 210
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
FT ACT_SITE 234
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
FT ACT_SITE 306
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
FT ACT_SITE 309
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
FT ACT_SITE 332
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
FT ACT_SITE 341
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000250|UniProtKB:P0A8P8, ECO:0000255|HAMAP-
FT Rule:MF_01816"
SQ SEQUENCE 356 AA; 41171 MW; 1E3C68618B650155 CRC64;
MKREILLERI DKLKQLMPWY VLEYYQSKLA VPYSFTTLYE YLKEYDRFFS WVLESGISNA
DKISDIPLSV LENMSKKDME SFILYLRERP LLNANTTKQG VSQTTINRTL SALSSLYKYL
TEEVENDQGE PYFYRNVMKK VSTKKKKETL AARAENIKQK LFLGDETEGF LTYIDQEHPQ
QLSNRALSSF NKNKERDLAI IALLLASGVR LSEAVNLDLR DLNLKMMVID VTRKGGKRDS
VNVAAFAKPY LENYLAIRNQ RYKTEKTDTA LFLTLYRGVP NRIDASSVEK MVAKYSEDFK
VRVTPHKLRH TLATRLYDAT KSQVLVSHQL GHASTQVTDL YTHIVNDEQK NALDSL
