XG74_ACETH
ID XG74_ACETH Reviewed; 842 AA.
AC Q70DK5;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Xyloglucanase Xgh74A {ECO:0000303|PubMed:16207921};
DE EC=3.2.1.-;
DE Flags: Precursor;
GN Name=xghA {ECO:0000312|EMBL:CAE51306.1};
OS Acetivibrio thermocellus (Hungateiclostridium thermocellum) (Clostridium
OS thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=1515;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAE51306.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=F7 {ECO:0000312|EMBL:CAE51306.1};
RX PubMed=16207921; DOI=10.1099/mic.0.28206-0;
RA Zverlov V.V., Schantz N., Schmitt-Kopplin P., Schwarz W.H.;
RT "Two new major subunits in the cellulosome of Clostridium thermocellum:
RT xyloglucanase Xgh74A and endoxylanase Xyn10D.";
RL Microbiology 151:3395-3401(2005).
RN [2] {ECO:0000305}
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-764, FUNCTION, ACTIVE SITES,
RP AND MUTAGENESIS OF ASP-70 AND ASP-480.
RX PubMed=16772298; DOI=10.1074/jbc.m603583200;
RA Martinez-Fleites C., Guerreiro C.I., Baumann M.J., Taylor E.J.,
RA Prates J.A., Ferreira L.M., Fontes C.M., Brumer H., Davies G.J.;
RT "Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A,
RT reveal the structural basis for xyloglucan recognition and degradation.";
RL J. Biol. Chem. 281:24922-24933(2006).
CC -!- FUNCTION: Hydrolyzes the glucosidic bonds of unbranched Glc residues in
CC tamarind seed xyloglucan, producing XXXG, XLXG, XXLG and XLLG. Has low
CC activity on carboxymethylcellulose, lichenan,hydroxyethylcellulose and
CC glucuronoxylan, and no activity on xylan, polygalaturonic acid, wheat
CC arabinoxylan, rhamnogalacturan, curdlan, laminarin, galactomannan,
CC galactan, arabinan and pachyman or amorphous cellulose.
CC {ECO:0000269|PubMed:16207921, ECO:0000269|PubMed:16772298}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6.4 with tamarind seed xyloglucan as substrate. Retains
CC 50% of its activity between pH 5.7 and 7.8.
CC {ECO:0000269|PubMed:16207921};
CC Temperature dependence:
CC Optimum temperature is 75 degrees Celsius. Retains 25% of its
CC activity after incubation at 90 degrees Celsius for 30 minutes.
CC {ECO:0000269|PubMed:16207921};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 74 family.
CC {ECO:0000269|PubMed:16207921}.
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DR EMBL; AJ585344; CAE51306.1; -; Genomic_DNA.
DR PDB; 2CN2; X-ray; 2.10 A; A/B/C/D=28-764.
DR PDB; 2CN3; X-ray; 1.95 A; A/B=28-764.
DR PDBsum; 2CN2; -.
DR PDBsum; 2CN3; -.
DR AlphaFoldDB; Q70DK5; -.
DR SMR; Q70DK5; -.
DR IntAct; Q70DK5; 1.
DR CAZy; GH74; Glycoside Hydrolase Family 74.
DR BRENDA; 3.2.1.155; 1530.
DR EvolutionaryTrace; Q70DK5; -.
DR GO; GO:0043263; C:cellulosome; IDA:MENGO.
DR GO; GO:0033946; F:xyloglucan-specific endo-beta-1,4-glucanase activity; IDA:MENGO.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:2000899; P:xyloglucan catabolic process; IDA:MENGO.
DR Gene3D; 1.10.1330.10; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR002105; Dockerin_1_rpt.
DR InterPro; IPR016134; Dockerin_dom.
DR InterPro; IPR036439; Dockerin_dom_sf.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF00404; Dockerin_1; 1.
DR SUPFAM; SSF63446; SSF63446; 1.
DR PROSITE; PS00448; CLOS_CELLULOSOME_RPT; 2.
DR PROSITE; PS51766; DOCKERIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Cellulose degradation; Glycosidase;
KW Hydrolase; Polysaccharide degradation; Repeat; Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..842
FT /note="Xyloglucanase Xgh74A"
FT /evidence="ECO:0000255"
FT /id="PRO_0000395870"
FT REPEAT 134..144
FT /note="BNR 1"
FT /evidence="ECO:0000255"
FT REPEAT 185..196
FT /note="BNR 2"
FT /evidence="ECO:0000255"
FT REPEAT 252..262
FT /note="BNR 3"
FT /evidence="ECO:0000255"
FT REPEAT 358..368
FT /note="BNR 4"
FT /evidence="ECO:0000255"
FT REPEAT 533..541
FT /note="BNR 5"
FT /evidence="ECO:0000255"
FT REPEAT 577..586
FT /note="BNR 6"
FT /evidence="ECO:0000255"
FT REPEAT 616..626
FT /note="BNR 7"
FT /evidence="ECO:0000255"
FT REPEAT 660..671
FT /note="BNR 8"
FT /evidence="ECO:0000255"
FT REPEAT 708..718
FT /note="BNR 9"
FT /evidence="ECO:0000255"
FT DOMAIN 771..841
FT /note="Dockerin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01102"
FT ACT_SITE 70
FT /note="Nucleophile"
FT /evidence="ECO:0000269|PubMed:16772298"
FT ACT_SITE 480
FT /note="Proton donor"
FT /evidence="ECO:0000269|PubMed:16772298"
FT MUTAGEN 70
FT /note="D->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:16772298"
FT MUTAGEN 480
FT /note="D->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:16772298"
FT STRAND 38..43
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 65..68
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 70..72
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:2CN3"
FT TURN 79..82
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:2CN3"
FT HELIX 93..99
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 101..106
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 114..118
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 122..126
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 131..141
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:2CN3"
FT TURN 156..159
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 174..177
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 184..189
FT /evidence="ECO:0007829|PDB:2CN3"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 221..226
FT /evidence="ECO:0007829|PDB:2CN3"
FT HELIX 228..230
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 240..245
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 251..256
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 270..278
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 282..291
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 301..307
FT /evidence="ECO:0007829|PDB:2CN3"
FT TURN 308..310
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 328..335
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 343..348
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 357..362
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 368..373
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 375..377
FT /evidence="ECO:0007829|PDB:2CN2"
FT STRAND 379..386
FT /evidence="ECO:0007829|PDB:2CN3"
FT HELIX 391..394
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 415..417
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 425..428
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 433..437
FT /evidence="ECO:0007829|PDB:2CN3"
FT HELIX 439..444
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 448..452
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 461..466
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 469..471
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 473..478
FT /evidence="ECO:0007829|PDB:2CN3"
FT TURN 479..481
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 485..488
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 502..510
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 513..522
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 530..536
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 555..559
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 566..569
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 576..580
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 596..599
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 607..611
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 614..620
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 638..643
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 646..653
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 659..664
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 676..684
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 694..701
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 704..712
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 734..737
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 740..747
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 749..751
FT /evidence="ECO:0007829|PDB:2CN3"
FT STRAND 753..758
FT /evidence="ECO:0007829|PDB:2CN3"
SQ SEQUENCE 842 AA; 92393 MW; CC686932DBA95FD3 CRC64;
MVKKFTSKIK AAVFAAVVAA TAIFGPAISS QAVTSVPYKW DNVVIGGGGG FMPGIVFNET
EKDLIYARAD IGGAYRWDPS TETWIPLLDH FQMDEYSYYG VESIATDPVD PNRVYIVAGM
YTNDWLPNMG AILRSTDRGE TWEKTILPFK MGGNMPGRSM GERLAIDPND NRILYLGTRC
GNGLWRSTDY GVTWSKVESF PNPGTYIYDP NFDYTKDIIG VVWVVFDKSS STPGNPTKTI
YVGVADKNES IYRSTDGGVT WKAVPGQPKG LLPHHGVLAS NGMLYITYGD TCGPYDGNGK
GQVWKFNTRT GEWIDITPIP YSSSDNRFCF AGLAVDRQNP DIIMVTSMNA WWPDEYIFRS
TDGGATWKNI WEWGMYPERI LHYEIDISAA PWLDWGTEKQ LPEINPKLGW MIGDIEIDPF
NSDRMMYVTG ATIYGCDNLT DWDRGGKVKI EVKATGIEEC AVLDLVSPPE GAPLVSAVGD
LVGFVHDDLK VGPKKMHVPS YSSGTGIDYA ELVPNFMALV AKADLYDVKK ISFSYDGGRN
WFQPPNEAPN SVGGGSVAVA ADAKSVIWTP ENASPAVTTD NGNSWKVCTN LGMGAVVASD
RVNGKKFYAF YNGKFYISTD GGLTFTDTKA PQLPKSVNKI KAVPGKEGHV WLAAREGGLW
RSTDGGYTFE KLSNVDTAHV VGFGKAAPGQ DYMAIYITGK IDNVLGFFRS DDAGKTWVRI
NDDEHGYGAV DTAITGDPRV YGRVYIATNG RGIVYGEPAS DEPVPTPPQV DKGLVGDLNG
DNRINSTDLT LMKRYILKSI EDLPVEDDLW AADINGDGKI NSTDYTYLKK YLLQAIPELP
KK
