ID   CAP13_ROSEK             Reviewed;         234 AA.
AC   A0A150XSR0;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2021, sequence version 2.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=CD-NTase-associated protein 13 {ECO:0000305};
DE            Short=Cap13 {ECO:0000305};
DE   AltName: Full=TM-STING {ECO:0000303|PubMed:32877915};
DE            Short=ReSTING {ECO:0000303|PubMed:32877915};
GN   Name=cap13 {ECO:0000305}; ORFNames=MB14_13770;
OS   Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB
OS   14502 / KMM 6017).
OC   Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Roseivirgaceae;
OC   Roseivirga.
OX   NCBI_TaxID=279360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017;
RA   Selvaratnam C., Thevarajoo S., Goh K.M., Ee R., Chan K.-G., Chong C.S.;
RT   "Genome sequencing of Roseivirga ehrenbergii KMM 6017.";
RL   Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   C-DI-GMP-BINDING, NUCLEOTIDE-BINDING, AND DOMAIN.
RX   PubMed=32877915; DOI=10.1038/s41586-020-2719-5;
RA   Morehouse B.R., Govande A.A., Millman A., Keszei A.F.A., Lowey B., Ofir G.,
RA   Shao S., Sorek R., Kranzusch P.J.;
RT   "STING cyclic dinucleotide sensing originated in bacteria.";
RL   Nature 586:429-433(2020).
RN   [3]
RP   CLASSIFICATION AND NOMENCLATURE.
RX   PubMed=32839535; DOI=10.1038/s41564-020-0777-y;
RA   Millman A., Melamed S., Amitai G., Sorek R.;
RT   "Diversity and classification of cyclic-oligonucleotide-based anti-phage
RT   signalling systems.";
RL   Nat. Microbiol. 5:1608-1615(2020).
CC   -!- FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling
CC       system) provides immunity against bacteriophage. The CD-NTase protein
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate a diverse range
CC       of effectors, leading to bacterial cell death and thus abortive phage
CC       infection. A type I-D CBASS(GG) system (PubMed:32839535).
CC       {ECO:0000303|PubMed:32839535, ECO:0000305|PubMed:32877915}.
CC   -!- FUNCTION: Binds c-di-GMP (synthesized by the cognate CdnE encoded
CC       upstream in the same operon) and about 10-fold less well 3'3'-cGAMP,
CC       but not c-di-AMP, 2'3'-cGAMP or cUMP-AMP (tested with a protein without
CC       the transmembrane region) (PubMed:32877915). The effector protein for
CC       this CBASS system, its activity is stimulated by c-di-GMP and leads to
CC       cell death (Probable). {ECO:0000269|PubMed:32877915,
CC       ECO:0000305|PubMed:32877915}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The cyclic nucleotide binds in the C-terminal bacterial STING
CC       region. {ECO:0000305|PubMed:32877915}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the bacterial STING
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=KYG81645.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; LQZQ01000002; KYG81645.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A0A150XSR0; -.
DR   SMR; A0A150XSR0; -.
DR   EnsemblBacteria; KYG81645; KYG81645; MB14_13770.
DR   Proteomes; UP000075583; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..234
FT                   /note="CD-NTase-associated protein 13"
FT                   /id="PRO_0000451883"
FT   TRANSMEM        20..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   234 AA;  27006 MW;  0881E0EA267D7832 CRC64;
     MKYGLLQENG KVAEFIQIKT IMKNVIANVS TAITLALMIL WIKYPNRIEW EAIIGILLVI
     KEVTIRWQIG KIESLEFSPA ISLAHGYVNN FLEPAINELL MKASNNINFS IYIPHDLEEL
     SDQQIDRMKL QIEANGYRLK EIKLKKKTGR PHDLLLVEKQ EGTLSYFDFP RTLLSLQSYI
     DYKVDSTKNE FSEEKKIAMG AKLVDAFHNE VDRLIKKKNL EGIVTFVSKD LELY