ID   XGEA_ASPOR              Reviewed;         240 AA.
AC   Q2UK93;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Probable xyloglucan-specific endo-beta-1,4-glucanase A;
DE            EC=3.2.1.151;
DE   AltName: Full=Xyloglucanase A;
DE   AltName: Full=Xyloglucanendohydrolase A;
DE   Flags: Precursor;
GN   Name=xgeA; ORFNames=AO090003000905;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in
CC       xyloglucan with retention of the beta-configuration of the glycosyl
CC       residues. Specific for xyloglucan and does not hydrolyze other cell
CC       wall components (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=xyloglucan + H2O = xyloglucan oligosaccharides.; EC=3.2.1.151;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 12 (cellulase H) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP007155; BAE58022.1; -; Genomic_DNA.
DR   RefSeq; XP_001820024.1; XM_001819972.2.
DR   AlphaFoldDB; Q2UK93; -.
DR   SMR; Q2UK93; -.
DR   CAZy; GH12; Glycoside Hydrolase Family 12.
DR   EnsemblFungi; BAE58022; BAE58022; AO090003000905.
DR   GeneID; 5992007; -.
DR   KEGG; aor:AO090003000905; -.
DR   VEuPathDB; FungiDB:AO090003000905; -.
DR   HOGENOM; CLU_051064_0_1_1; -.
DR   OMA; KSLPSVW; -.
DR   Proteomes; UP000006564; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:InterPro.
DR   GO; GO:0033946; F:xyloglucan-specific endo-beta-1,4-glucanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.180; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013319; GH11/12.
DR   InterPro; IPR002594; GH12.
DR   PANTHER; PTHR34002; PTHR34002; 1.
DR   Pfam; PF01670; Glyco_hydro_12; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..240
FT                   /note="Probable xyloglucan-specific endo-beta-1,4-glucanase
FT                   A"
FT                   /id="PRO_0000394073"
SQ   SEQUENCE   240 AA;  25595 MW;  2BA49C17A2676DEA CRC64;
     MKFLTPLVLS SLASAAALNR RADMCGQWDT TTTDKFTLYN NLWGEGNADS GSQCTGLDSD
     DGNTIAWHTS WTWTGGAGQV KSFANVAYNF EATQLSQLSS IPSTWKWENT GSDIVADVAY
     DLFTSSSADG DEEYEIMIWL AALGGAGPIS STGSAIATPT VGGQSWSLYS GPNGQMTVFS
     FVASSTTEDF SADLNDFLKY LQEEQGMPSS QYLTHVQAGT EPFSGSNVKF TTSSYSVSVA