ID   CAP18_RABIT             Reviewed;         171 AA.
AC   P25230;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Antimicrobial protein CAP18;
DE   AltName: Full=18 kDa cationic protein;
DE   AltName: Full=18 kDa lipopolysaccharide-binding protein;
DE   AltName: Full=CAP18-A;
DE   Contains:
DE     RecName: Full=Antimicrobial protein CAP7;
DE   Flags: Precursor;
GN   Name=CAP18;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 135-159.
RC   TISSUE=Bone marrow;
RX   PubMed=1883348; DOI=10.1016/0006-291x(91)91350-l;
RA   Larrick J.W., Morgan J.G., Palings I., Hirata M., Yen M.H.;
RT   "Complementary DNA sequence of rabbit CAP18 -- a unique lipopolysaccharide
RT   binding protein.";
RL   Biochem. Biophys. Res. Commun. 179:170-175(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 135-159, AND CHARACTERIZATION.
RX   PubMed=8132348; DOI=10.1128/iai.62.4.1421-1426.1994;
RA   Hirata M., Shimomura Y., Yoshida M., Morgan J.G., Palings I., Wilson D.,
RA   Yen M.H., Wright S.C., Larrick J.W.;
RT   "Characterization of a rabbit cationic protein (CAP18) with
RT   lipopolysaccharide-inhibitory activity.";
RL   Infect. Immun. 62:1421-1426(1994).
RN   [3]
RP   PROTEIN SEQUENCE OF 135-154, AND CHARACTERIZATION.
RX   PubMed=8254193;
RA   Larrick J.W., Hirata M., Zheng H., Zhong J., Bolin D., Cavaillon J.-M.,
RA   Warren H.S., Wright S.C.;
RT   "A novel granulocyte-derived peptide with lipopolysaccharide-neutralizing
RT   activity.";
RL   J. Immunol. 152:231-240(1994).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8313956; DOI=10.1016/0014-5793(94)80395-1;
RA   Tossi A., Scocchi M., Skerlavaj B., Gennaro R.;
RT   "Identification and characterization of a primary antibacterial domain in
RT   CAP18, a lipopolysaccharide binding protein from rabbit leukocytes.";
RL   FEBS Lett. 339:108-112(1994).
RN   [5]
RP   STRUCTURE BY NMR OF 135-166.
RX   PubMed=7649303; DOI=10.1016/0014-5793(95)00792-8;
RA   Chen C., Brock R., Luh F., Chou P.-J., Larrick J.W., Huang R.-F.,
RA   Huang T.-H.;
RT   "The solution structure of the active domain of CAP18 -- a
RT   lipopolysaccharide binding protein from rabbit leukocytes.";
RL   FEBS Lett. 370:46-52(1995).
CC   -!- FUNCTION: CAP18 binds to the lipid A moiety of bacterial
CC       lipopolysaccharides (LPS), a glycolipid present in the outer membrane
CC       of all Gram-negative bacteria. Has antibiotic activity.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Neutrophils.
CC   -!- SIMILARITY: Belongs to the cathelicidin family. {ECO:0000305}.
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DR   EMBL; M73998; AAA31187.1; -; mRNA.
DR   PIR; JQ1171; JQ1171.
DR   RefSeq; NP_001075774.1; NM_001082305.1.
DR   PDB; 1LYP; NMR; -; A=135-166.
DR   PDBsum; 1LYP; -.
DR   AlphaFoldDB; P25230; -.
DR   SMR; P25230; -.
DR   STRING; 9986.ENSOCUP00000013325; -.
DR   TCDB; 1.C.33.1.6; the cathelicidin (cathelicidin) family.
DR   GeneID; 100009142; -.
DR   KEGG; ocu:100009142; -.
DR   CTD; 820; -.
DR   eggNOG; ENOG502SAES; Eukaryota.
DR   InParanoid; P25230; -.
DR   OrthoDB; 1534863at2759; -.
DR   EvolutionaryTrace; P25230; -.
DR   Proteomes; UP000001811; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR001894; Cathelicidin-like.
DR   InterPro; IPR018216; Cathelicidin_CS.
DR   InterPro; IPR022746; Cathlecidin_C.
DR   InterPro; IPR046350; Cystatin_sf.
DR   PANTHER; PTHR10206; PTHR10206; 1.
DR   Pfam; PF12153; CAP18_C; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00946; CATHELICIDINS_1; 1.
DR   PROSITE; PS00947; CATHELICIDINS_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..171
FT                   /note="Antimicrobial protein CAP18"
FT                   /id="PRO_0000004754"
FT   CHAIN           135..171
FT                   /note="Antimicrobial protein CAP7"
FT                   /id="PRO_0000004755"
FT   DISULFID        85..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        107..124
FT                   /evidence="ECO:0000250"
FT   VARIANT         157
FT                   /note="K -> D"
FT   HELIX           136..165
FT                   /evidence="ECO:0007829|PDB:1LYP"
SQ   SEQUENCE   171 AA;  19805 MW;  D7BF2103BCFB13C4 CRC64;
     METHKHGPSL AWWSLLLLLL GLLMPPAIAQ DLTYREAVLR AVDAFNQQSS EANLYRLLSM
     DPQQLEDAKP YTPQPVSFTV KETECPRTTW KLPEQCDFKE DGLVKRCVGT VTRYQAWDSF
     DIRCNRAQES PEPTGLRKRL RKFRNKIKEK LKKIGQKIQG FVPKLAPRTD Y