CAP2_ARATH
ID CAP2_ARATH Reviewed; 653 AA.
AC Q8LF20; Q9SKK6;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Putative clathrin assembly protein At2g25430;
GN OrderedLocusNames=At2g25430; ORFNames=F13B15.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Root;
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-244, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBCELLULAR LOCATION: Membrane, clathrin-coated pit {ECO:0000250}.
CC Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000250}. Note=Colocalized with clathrin in the Golgi
CC area. {ECO:0000250}.
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DR EMBL; AC006300; AAD20703.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07701.1; -; Genomic_DNA.
DR EMBL; AY139760; AAM98081.1; -; mRNA.
DR EMBL; BT008299; AAP37658.1; -; mRNA.
DR EMBL; AY085092; AAM61646.1; -; mRNA.
DR PIR; C84648; C84648.
DR RefSeq; NP_565595.1; NM_128100.4.
DR AlphaFoldDB; Q8LF20; -.
DR SMR; Q8LF20; -.
DR BioGRID; 2433; 2.
DR STRING; 3702.AT2G25430.1; -.
DR iPTMnet; Q8LF20; -.
DR PaxDb; Q8LF20; -.
DR PRIDE; Q8LF20; -.
DR ProteomicsDB; 239180; -.
DR EnsemblPlants; AT2G25430.1; AT2G25430.1; AT2G25430.
DR GeneID; 817081; -.
DR Gramene; AT2G25430.1; AT2G25430.1; AT2G25430.
DR KEGG; ath:AT2G25430; -.
DR Araport; AT2G25430; -.
DR TAIR; locus:2040115; AT2G25430.
DR eggNOG; KOG0251; Eukaryota.
DR HOGENOM; CLU_014098_3_0_1; -.
DR InParanoid; Q8LF20; -.
DR OMA; FVRTFAM; -.
DR OrthoDB; 388752at2759; -.
DR PhylomeDB; Q8LF20; -.
DR PRO; PR:Q8LF20; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LF20; baseline and differential.
DR Genevisible; Q8LF20; AT.
DR GO; GO:0005905; C:clathrin-coated pit; IBA:GO_Central.
DR GO; GO:0030136; C:clathrin-coated vesicle; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0005545; F:1-phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0032050; F:clathrin heavy chain binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0048268; P:clathrin coat assembly; IEA:InterPro.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IBA:GO_Central.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR Gene3D; 1.20.58.150; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR011417; ANTH_dom.
DR InterPro; IPR014712; ANTH_dom_sf.
DR InterPro; IPR045192; AP180-like.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR PANTHER; PTHR22951; PTHR22951; 1.
DR Pfam; PF07651; ANTH; 2.
DR SMART; SM00273; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
PE 1: Evidence at protein level;
KW Coated pit; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..653
FT /note="Putative clathrin assembly protein At2g25430"
FT /id="PRO_0000187068"
FT DOMAIN 23..159
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT REGION 160..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 376..431
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 173..190
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..393
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..428
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 221
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8S9J8"
FT MOD_RES 244
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 203
FT /note="Missing (in Ref. 4; AAM61646)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="G -> GG (in Ref. 4; AAM61646)"
FT /evidence="ECO:0000305"
FT CONFLICT 501
FT /note="T -> A (in Ref. 4; AAM61646)"
FT /evidence="ECO:0000305"
FT CONFLICT 631
FT /note="A -> T (in Ref. 4; AAM61646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 653 AA; 72084 MW; A61B21EBA98206D0 CRC64;
MAPSIRKAIG AVKDQTSIGI AKVASNMAPD LEVAIVKATS HDDDPASEKY IREILNLTSL
SRGYILACVT SVSRRLSKTR DWVVALKALM LVHRLLNEGD PIFQEEILYS TRRGTRMLNM
SDFRDEAHSS SWDHSAFVRT YAGYLDQRLE LALFERKSGV SVNSGGNSSH HSNNDDRYGR
GRDDFRSPPP RSYDYENGGG GGSDFRGDNN GYGGVPKRSR SYGDMTEMGG GGGGGGRDEK
KVVTPLREMT PERIFGKMGH LQRLLDRFLS LRPTGLAKNS RMILIALYPV VRESFKLYAD
ICEVLAVLLD KFFDMEYSDC VKAFDAYASA AKQIDELIAF YNWCKETGVA RSSEYPEVQR
ITSKLLETLE EFVRDRAKRG KSPERKEIEA PPPVVEEEEP EPDMNEIKAL PPPENYTPPP
PPEPEPQPEK PQFTEDLVNL REDEVTADDQ GNKFALALFA GPPGNNGKWE AFSSNGVTSA
WQNPAAEPGK ADWELALVET TSNLEKQTAA LGGGFDNLLL NGMYDQGMVR QHVSTSQLTG
GSASSVALPL PGKTNNQVLA LPAPDGTVEK VNQDPFAASL TIPPPSYVQM AEMEKKQYLL
SQEQQLWQQY QRDGMRGQAS LAKMNTGPVP AYGMPPVNGM GPPPTGYYYN NPY
