ID   XISA_NOSS1              Reviewed;         472 AA.
AC   P08862; Q44136;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 3.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Excisase A;
DE   AltName: Full=NifD element site-specific recombinase;
GN   Name=xisA; OrderedLocusNames=alr1442;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3006922; DOI=10.1016/0092-8674(86)90013-9;
RA   Lammers P.J., Golden J.W., Haselkorn R.;
RT   "Identification and sequence of a gene required for a developmentally
RT   regulated DNA excision in Anabaena.";
RL   Cell 44:905-911(1986).
RN   [2]
RP   SEQUENCE REVISION.
RA   Carrasco C.D., Golden J.W.;
RL   Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [4]
RP   FUNCTION.
RX   PubMed=3144039; DOI=10.1126/science.3144039;
RA   Golden J.W., Wiest D.R.;
RT   "Genome rearrangement and nitrogen fixation in Anabaena blocked by
RT   inactivation of xisA gene.";
RL   Science 242:1421-1423(1988).
CC   -!- FUNCTION: Essential for DNA excision. Site specific recombinase
CC       necessary for the excision of the 11 kb nifD element during heterocyst
CC       differentiation. {ECO:0000269|PubMed:3144039}.
CC   -!- SIMILARITY: Belongs to the XisA/XisC recombinase family. {ECO:0000305}.
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DR   EMBL; U38537; AAC82961.1; -; Genomic_DNA.
DR   EMBL; BA000019; BAB73399.1; -; Genomic_DNA.
DR   PIR; A24782; A24782.
DR   PIR; AG1986; AG1986.
DR   AlphaFoldDB; P08862; -.
DR   STRING; 103690.17130789; -.
DR   EnsemblBacteria; BAB73399; BAB73399; BAB73399.
DR   KEGG; ana:alr1442; -.
DR   eggNOG; COG0582; Bacteria.
DR   OMA; SEHTFFY; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IDA:CACAO.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IMP:CACAO.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0043158; P:heterocyst differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IMP:CACAO.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA excision; DNA recombination; Heterocyst; Reference proteome.
FT   CHAIN           1..472
FT                   /note="Excisase A"
FT                   /id="PRO_0000066009"
FT   DOMAIN          244..429
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        287
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        317
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        384
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        407
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        416
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   CONFLICT        55
FT                   /note="L -> S (in Ref. 1; AAC82961)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        82
FT                   /note="G -> R (in Ref. 1; AAC82961)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        166..167
FT                   /note="SE -> NK (in Ref. 1; AAC82961)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   472 AA;  55228 MW;  2A0E52A76919CD94 CRC64;
     MQNQGQDKYQ QAFADLEPLS STDGSFLGSS LQAQQQREHM RTKVLQDLDK VNLRLKSAKT
     KVSVRESNGS LQLRATLPIK PGDKDTNGTG RKQYNLSLNI PANLDGLKTA EEEAYELGKL
     IARKTFEWND KYLGKEATKK DSQTIGDLLE KFAEEYFKTH KRTTKSEHTF FYYFSRTQRY
     TNSKDLATAE NLINSIEQID KEWARYNAAR AISAFCITFN IEIDLSQYSK MPDRNSRNIP
     TDAEILSGIT KFEDYLVTRG NQVNEDVKDS WQLWRWTYGM LAVFGLRPRE IFINPNIDWW
     LSKENIDLTW KVDKECKTGE RQALPLHKEW IDEFDLRNPK YLEMLATAIS KKDKTNHAEI
     TALTQRISWW FRKVELDFKP YDLRHAWAIR AHILGIPIKA AADNLGHSMQ VHTQTYQRWF
     SLDMRKLAIN QALTKRNEFE VIREENAKLQ IENERLRMEI EKLKMEIAYK NS