ID   XIS_BACSU               Reviewed;          64 AA.
AC   O31490;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=ICEBs1 excisionase;
DE   AltName: Full=Recombination directionality factor xis;
GN   Name=xis; Synonyms=sacV; OrderedLocusNames=BSU04830;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   FUNCTION.
RX   PubMed=18005101; DOI=10.1111/j.1365-2958.2007.06000.x;
RA   Lee C.A., Auchtung J.M., Monson R.E., Grossman A.D.;
RT   "Identification and characterization of int (integrase), xis (excisionase)
RT   and chromosomal attachment sites of the integrative and conjugative element
RT   ICEBs1 of Bacillus subtilis.";
RL   Mol. Microbiol. 66:1356-1369(2007).
RN   [3]
RP   INDUCTION.
RX   PubMed=16105942; DOI=10.1073/pnas.0505835102;
RA   Auchtung J.M., Lee C.A., Monson R.E., Lehman A.P., Grossman A.D.;
RT   "Regulation of a Bacillus subtilis mobile genetic element by intercellular
RT   signaling and the global DNA damage response.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12554-12559(2005).
RN   [4]
RP   INDUCTION.
RX   PubMed=17511812; DOI=10.1111/j.1365-2958.2007.05748.x;
RA   Auchtung J.M., Lee C.A., Garrison K.L., Grossman A.D.;
RT   "Identification and characterization of the immunity repressor (ImmR) that
RT   controls the mobile genetic element ICEBs1 of Bacillus subtilis.";
RL   Mol. Microbiol. 64:1515-1528(2007).
CC   -!- FUNCTION: Required for the excision of the integrative and conjugative
CC       element ICEBs1. Excision of ICEBs1 requires two sites, attL and attR,
CC       at the left and right ends of the integrated ICEBs1.
CC       {ECO:0000269|PubMed:18005101}.
CC   -!- INDUCTION: Constitutively expressed at very low levels. Induced in
CC       response to overproduction of RapI or treatment with DNA-damaging agent
CC       mitomycin C (MMC). {ECO:0000269|PubMed:16105942,
CC       ECO:0000269|PubMed:17511812}.
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DR   EMBL; AL009126; CAB12290.1; -; Genomic_DNA.
DR   PIR; D69703; D69703.
DR   RefSeq; NP_388364.1; NC_000964.3.
DR   RefSeq; WP_003240395.1; NZ_JNCM01000031.1.
DR   AlphaFoldDB; O31490; -.
DR   STRING; 224308.BSU04830; -.
DR   TCDB; 3.A.7.14.3; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR   PaxDb; O31490; -.
DR   PRIDE; O31490; -.
DR   EnsemblBacteria; CAB12290; CAB12290; BSU_04830.
DR   GeneID; 939938; -.
DR   KEGG; bsu:BSU04830; -.
DR   PATRIC; fig|224308.179.peg.513; -.
DR   OMA; TADICEL; -.
DR   BioCyc; BSUB:BSU04830-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   DNA integration; DNA recombination; DNA-binding; Reference proteome.
FT   CHAIN           1..64
FT                   /note="ICEBs1 excisionase"
FT                   /id="PRO_0000360641"
SQ   SEQUENCE   64 AA;  7309 MW;  C0B1481D1269C8F5 CRC64;
     MKGEFLTARD IQKILGVKQA KSYDIIRTLN AQMKEEGYMV IQGKVSRAKF EECYCYKGPK
     SQTG