ID   CAP2_ECOLX              Reviewed;         589 AA.
AC   P0DTF2;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2021, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=CD-NTase-associated protein 2 {ECO:0000303|PubMed:32544385};
GN   Name=cap2 {ECO:0000303|PubMed:32544385};
GN   ORFNames=ESG_RS0100130, geneC {ECO:0000303|PubMed:31533127};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TW11681;
RX   PubMed=21078854; DOI=10.1128/iai.00932-10;
RA   Sahl J.W., Steinsland H., Redman J.C., Angiuoli S.V., Nataro J.P.,
RA   Sommerfelt H., Rasko D.A.;
RT   "A comparative genomic analysis of diverse clonal types of enterotoxigenic
RT   Escherichia coli reveals pathovar-specific conservation.";
RL   Infect. Immun. 79:950-960(2011).
RN   [2]
RP   ANTIVIRAL DEFENSE, OPERON STRUCTURE, AND MUTAGENESIS OF 493-CYS--CYS-496.
RC   STRAIN=TW11681;
RX   PubMed=31533127; DOI=10.1038/s41586-019-1605-5;
RA   Cohen D., Melamed S., Millman A., Shulman G., Oppenheimer-Shaanan Y.,
RA   Kacen A., Doron S., Amitai G., Sorek R.;
RT   "Cyclic GMP-AMP signalling protects bacteria against viral infection.";
RL   Nature 574:691-695(2019).
RN   [3]
RP   NOMENCLATURE.
RC   STRAIN=TW11681;
RX   PubMed=32544385; DOI=10.1016/j.cell.2020.05.019;
RA   Lowey B., Whiteley A.T., Keszei A.F.A., Morehouse B.R., Antine S.P.,
RA   Cabrera V.J., Kashin D., Schwede F., Mekalanos J.J., Shao S., Lee A.S.Y.,
RA   Kranzusch P.J.;
RT   "CBASS immunity uses CARF-related effectors to sense 3'-5' and 2'-5'-linked
RT   cyclic oligonucleotide signals and protect bacteria from phage infection.";
RL   Cell 182:38-49(2020).
RN   [4]
RP   CLASSIFICATION AND NOMENCLATURE.
RX   PubMed=32839535; DOI=10.1038/s41564-020-0777-y;
RA   Millman A., Melamed S., Amitai G., Sorek R.;
RT   "Diversity and classification of cyclic-oligonucleotide-based anti-phage
RT   signalling systems.";
RL   Nat. Microbiol. 5:1608-1615(2020).
CC   -!- FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling
CC       system) provides immunity against bacteriophage. The CD-NTase protein
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate a diverse range
CC       of effectors, leading to bacterial cell death and thus abortive phage
CC       infection. A type II-A(GA) CBASS system (PubMed:32839535).
CC       {ECO:0000269|PubMed:31533127, ECO:0000303|PubMed:32839535}.
CC   -!- FUNCTION: Protects E.coli against phage infection. When capV and dncV
CC       are introduced in E.coli MG1655 there is 1000-fold protection against
CC       phage P1; protection against other phage (T2, T4, T5, T6 and lambda-
CC       vir) requires the 2 subsequent genes. {ECO:0000269|PubMed:31533127}.
CC   -!- INDUCTION: Part of the CBASS operon consisting of capV-dncV-cap2-cap3.
CC       {ECO:0000305|PubMed:31533127}.
CC   -!- MISCELLANEOUS: The sequence of this protein is available under the NCBI
CC       RefSeq accession WP_000390951.1. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the HesA/MoeB/ThiF
CC       family. {ECO:0000305}.
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DR   EMBL; AELD01000001; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_000390951.1; NZ_VDIV01000086.1.
DR   AlphaFoldDB; P0DTF2; -.
DR   GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR032701; Prok-E2_B_dom.
DR   InterPro; IPR045886; ThiF/MoeB/HesA.
DR   InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR   InterPro; IPR035985; Ubiquitin-activating_enz.
DR   PANTHER; PTHR43267; PTHR43267; 1.
DR   Pfam; PF14461; Prok-E2_B; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   SUPFAM; SSF69572; SSF69572; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..589
FT                   /note="CD-NTase-associated protein 2"
FT                   /id="PRO_0000451856"
FT   MUTAGEN         493..496
FT                   /note="CYGC->AYGA: Loss of defense against phage T4, T5, T6
FT                   and lambda."
FT                   /evidence="ECO:0000269|PubMed:31533127"
SQ   SEQUENCE   589 AA;  66805 MW;  19C5FA8C318770AA CRC64;
     MEEGRLHRVM LSCGYSFTYA RNLPEKAILY SQNCQQGYYT KEYTTVAGDV KIALIIRADP
     FIELPIAYIL ELPEQFKDRL MPHISLEGFL CYVEQMEADW DSNNLEDTYR EVDAQIQRTL
     VNSVSAAMEG SNDKKELEGE FTAYWRPSES LFVLSNANRS TRLKTFISQS VRPNGSSQVE
     YITVEESSPE NSGKVISAWL KLRYLPKNSL KEYHISTHYI SVNPSRVAGM KWPPASFRDL
     LSWLGKADHN AKNKVVEYIK KEGKKRYVFL FDVLKQDTFG IYVEFDLKSI DLKRYKHSAK
     NSTAKLSTVL GGKSVCSMYQ RLGVVRADIE TLLSRNTRRE GSAKLSEKRI ALVGCGTIGG
     YLAELLLRNG AGCGKGHLHL YDDDLYKPSN FGRHTLSAHD FGRYKSLSLA RKLKDSVHLP
     TQIIGFEKQF SIRADLMQKY DIIIDATGRP PVSKRMASIV RTIALEKRPK IIHAFNDGNG
     RASKVFIDDG RSCYGCMVSN PEKYRNGIDS RFCHIDISRE KNKNCGSTYT PYDAAVSNIT
     ASLTQMAVLS TLDPELKWTY SEHMLEGGRS LKSQFLPRQP NCPICNEYE