XKR9_HUMAN
ID XKR9_HUMAN Reviewed; 373 AA.
AC Q5GH70; B2RNS9; B9EH74;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=XK-related protein 9 {ECO:0000305};
DE Short=hXKR9 {ECO:0000303|PubMed:25231987};
DE Contains:
DE RecName: Full=XK-related protein 9, processed form {ECO:0000250|UniProtKB:Q5GH62};
GN Name=XKR9 {ECO:0000303|PubMed:25231987, ECO:0000312|HGNC:HGNC:20937};
GN Synonyms=XRG9 {ECO:0000303|Ref.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Huang C.-H., Chen Y.;
RT "A superfamily of XK-related genes (XRG) widely expressed in vertebrates
RT and invertebrates.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=25231987; DOI=10.1074/jbc.m114.583419;
RA Suzuki J., Imanishi E., Nagata S.;
RT "Exposure of phosphatidylserine by Xk-related protein family members during
RT apoptosis.";
RL J. Biol. Chem. 289:30257-30267(2014).
CC -!- FUNCTION: [XK-related protein 9, processed form]: Phospholipid
CC scramblase that promotes phosphatidylserine exposure on apoptotic cell
CC surface (PubMed:25231987). Phosphatidylserine is a specific marker only
CC present at the surface of apoptotic cells and acts as a specific signal
CC for engulfment (PubMed:25231987). {ECO:0000269|PubMed:25231987}.
CC -!- CATALYTIC ACTIVITY: [XK-related protein 9, processed form]:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-
CC sn-glycero-3-phospho-L-serine(out); Xref=Rhea:RHEA:38663,
CC ChEBI:CHEBI:57262; Evidence={ECO:0000305|PubMed:25231987};
CC -!- ACTIVITY REGULATION: Activated upon caspase cleavage to generate the
CC XK-related protein 9, processed form. Does not act prior the onset of
CC apoptosis. {ECO:0000250|UniProtKB:Q5GH62}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q5GH62};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- PTM: [XK-related protein 9]: Undergoes proteolytic processing by
CC caspase-3 (CASP3), caspase-6 (CASP6) and caspase-7 (CASP7) to generate
CC the XK-related protein 9, processed form, leading to its activation.
CC {ECO:0000250|UniProtKB:Q5GH62}.
CC -!- SIMILARITY: Belongs to the XK family. {ECO:0000305}.
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DR EMBL; AY534247; AAT07096.1; -; mRNA.
DR EMBL; BC137104; AAI37105.1; -; mRNA.
DR EMBL; BC137105; AAI37106.1; -; mRNA.
DR CCDS; CCDS34905.1; -.
DR RefSeq; NP_001011720.1; NM_001011720.1.
DR RefSeq; NP_001274187.1; NM_001287258.1.
DR RefSeq; NP_001274188.1; NM_001287259.1.
DR RefSeq; NP_001274189.1; NM_001287260.1.
DR RefSeq; XP_011515826.1; XM_011517524.2.
DR RefSeq; XP_011515827.1; XM_011517525.2.
DR RefSeq; XP_016868893.1; XM_017013404.1.
DR AlphaFoldDB; Q5GH70; -.
DR SMR; Q5GH70; -.
DR STRING; 9606.ENSP00000386141; -.
DR TCDB; 2.A.112.1.12; the kx blood-group antigen (kxa) family.
DR iPTMnet; Q5GH70; -.
DR PhosphoSitePlus; Q5GH70; -.
DR BioMuta; XKR9; -.
DR DMDM; 74707815; -.
DR MassIVE; Q5GH70; -.
DR PaxDb; Q5GH70; -.
DR PeptideAtlas; Q5GH70; -.
DR PRIDE; Q5GH70; -.
DR Antibodypedia; 51209; 7 antibodies from 6 providers.
DR DNASU; 389668; -.
DR Ensembl; ENST00000408926.8; ENSP00000386141.3; ENSG00000221947.8.
DR Ensembl; ENST00000520030.5; ENSP00000431088.1; ENSG00000221947.8.
DR GeneID; 389668; -.
DR KEGG; hsa:389668; -.
DR MANE-Select; ENST00000408926.8; ENSP00000386141.3; NM_001011720.2; NP_001011720.1.
DR UCSC; uc003xyq.3; human.
DR CTD; 389668; -.
DR DisGeNET; 389668; -.
DR GeneCards; XKR9; -.
DR HGNC; HGNC:20937; XKR9.
DR HPA; ENSG00000221947; Tissue enhanced (intestine, testis).
DR neXtProt; NX_Q5GH70; -.
DR OpenTargets; ENSG00000221947; -.
DR PharmGKB; PA142670567; -.
DR VEuPathDB; HostDB:ENSG00000221947; -.
DR eggNOG; KOG4790; Eukaryota.
DR GeneTree; ENSGT01050000244963; -.
DR HOGENOM; CLU_028534_2_0_1; -.
DR InParanoid; Q5GH70; -.
DR OMA; VSMEFLY; -.
DR OrthoDB; 1230316at2759; -.
DR PhylomeDB; Q5GH70; -.
DR TreeFam; TF316454; -.
DR PathwayCommons; Q5GH70; -.
DR BioGRID-ORCS; 389668; 15 hits in 1060 CRISPR screens.
DR ChiTaRS; XKR9; human.
DR GenomeRNAi; 389668; -.
DR Pharos; Q5GH70; Tdark.
DR PRO; PR:Q5GH70; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q5GH70; protein.
DR Bgee; ENSG00000221947; Expressed in duodenum and 97 other tissues.
DR ExpressionAtlas; Q5GH70; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0017128; F:phospholipid scramblase activity; ISS:UniProtKB.
DR GO; GO:1902742; P:apoptotic process involved in development; IBA:GO_Central.
DR GO; GO:0043652; P:engulfment of apoptotic cell; IBA:GO_Central.
DR GO; GO:0070782; P:phosphatidylserine exposure on apoptotic cell surface; ISS:UniProtKB.
DR InterPro; IPR018629; XK-rel.
DR Pfam; PF09815; XK-related; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..373
FT /note="XK-related protein 9"
FT /id="PRO_0000190796"
FT CHAIN 1..357
FT /note="XK-related protein 9, processed form"
FT /evidence="ECO:0000250|UniProtKB:Q5GH62"
FT /id="PRO_0000453293"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT SITE 357..358
FT /note="Cleavage; by caspase-3, caspase-6 and caspase-7"
FT /evidence="ECO:0000250|UniProtKB:Q5GH62"
SQ SEQUENCE 373 AA; 43406 MW; E6B9AF692FED626D CRC64;
MKYTKQNFMM SVLGIIIYVT DLIVDIWVSV RFFHEGQYVF SALALSFMLF GTLVAQCFSY
SWFKADLKKA GQESQHCFLL LHCLQGGVFT RYWFALKRGY HAAFKYDSNT SNFVEEQIDL
HKEVIDRVTD LSMLRLFETY LEGCPQLILQ LYILLEHGQA NFSQYAAIMV SCCAISWSTV
DYQVALRKSL PDKKLLNGLC PKITYLFYKL FTLLSWMLSV VLLLFLNVKI ALFLLLFLWL
LGIIWAFKNN TQFCTCISME FLYRIVVGFI LIFTFFNIKG QNTKCPMSCY YIVRVLGTLG
ILTVFWVCPL TIFNPDYFIP ISITIVLTLL LGILFLIVYY GSFHPNRSAE TKCDEIDGKP
VLRECRMRYF LME
