CAP2_VIBCH
ID CAP2_VIBCH Reviewed; 584 AA.
AC Q9KVG6;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=CD-NTase-associated protein 2 {ECO:0000303|PubMed:32544385};
GN Name=cap2 {ECO:0000303|PubMed:32544385};
GN OrderedLocusNames=VC_0180 {ECO:0000312|EMBL:AAF93356.1};
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [2]
RP ANTIVIRAL DEFENSE, AND OPERON STRUCTURE.
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=31533127; DOI=10.1038/s41586-019-1605-5;
RA Cohen D., Melamed S., Millman A., Shulman G., Oppenheimer-Shaanan Y.,
RA Kacen A., Doron S., Amitai G., Sorek R.;
RT "Cyclic GMP-AMP signalling protects bacteria against viral infection.";
RL Nature 574:691-695(2019).
RN [3]
RP ANTIVIRAL DEFENSE, NOMENCLATURE, AND OPERON STRUCTURE.
RC STRAIN=El Tor C6706;
RX PubMed=32544385; DOI=10.1016/j.cell.2020.05.019;
RA Lowey B., Whiteley A.T., Keszei A.F.A., Morehouse B.R., Antine S.P.,
RA Cabrera V.J., Kashin D., Schwede F., Mekalanos J.J., Shao S., Lee A.S.Y.,
RA Kranzusch P.J.;
RT "CBASS immunity uses CARF-related effectors to sense 3'-5' and 2'-5'-linked
RT cyclic oligonucleotide signals and protect bacteria from phage infection.";
RL Cell 182:38-49(2020).
RN [4]
RP CLASSIFICATION AND NOMENCLATURE.
RX PubMed=32839535; DOI=10.1038/s41564-020-0777-y;
RA Millman A., Melamed S., Amitai G., Sorek R.;
RT "Diversity and classification of cyclic-oligonucleotide-based anti-phage
RT signalling systems.";
RL Nat. Microbiol. 5:1608-1615(2020).
CC -!- FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling
CC system) provides immunity against bacteriophage. The CD-NTase protein
CC synthesizes cyclic nucleotides in response to infection; these serve as
CC specific second messenger signals. The signals activate a diverse range
CC of effectors, leading to bacterial cell death and thus abortive phage
CC infection. A type II-A(GA) CBASS system (PubMed:32839535).
CC {ECO:0000269|PubMed:31533127, ECO:0000303|PubMed:32839535}.
CC -!- FUNCTION: Protects E.coli against phage P1 and T2 infection. When the 4
CC gene operon capV-dncV-cap2-cap3 is introduced in E.coli MG1655 there is
CC about 100-fold protection against phages P1 and T2 (PubMed:31533127).
CC Protects E.coli against phage T2 infection. When the CBASS operon
CC (capV-dcnV-cap2-cap3) is introduced in E.coli MG1655 there is a more
CC than 10(3) decrease in the efficiency of T2 plaque formation. Protects
CC 100-fold against phage T5, offers no protection against T7
CC (PubMed:32544385). {ECO:0000269|PubMed:31533127,
CC ECO:0000269|PubMed:32544385}.
CC -!- INDUCTION: Part of the CBASS operon consisting of capV-dncV-cap2-cap3.
CC {ECO:0000305|PubMed:31533127, ECO:0000305|PubMed:32544385}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the HesA/MoeB/ThiF
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE003852; AAF93356.1; -; Genomic_DNA.
DR PIR; G82354; G82354.
DR RefSeq; NP_229837.1; NC_002505.1.
DR RefSeq; WP_001884104.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KVG6; -.
DR SMR; Q9KVG6; -.
DR STRING; 243277.VC_0180; -.
DR DNASU; 2614191; -.
DR EnsemblBacteria; AAF93356; AAF93356; VC_0180.
DR GeneID; 57738920; -.
DR KEGG; vch:VC_0180; -.
DR PATRIC; fig|243277.26.peg.164; -.
DR eggNOG; COG0476; Bacteria.
DR HOGENOM; CLU_032708_1_0_6; -.
DR OMA; RNINNEA; -.
DR BioCyc; VCHO:VC0180-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0061503; F:tRNA threonylcarbamoyladenosine dehydratase; IBA:GO_Central.
DR GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR GO; GO:0061504; P:cyclic threonylcarbamoyladenosine biosynthetic process; IBA:GO_Central.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR032701; Prok-E2_B_dom.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR PANTHER; PTHR43267; PTHR43267; 1.
DR Pfam; PF14461; Prok-E2_B; 1.
DR Pfam; PF00899; ThiF; 1.
DR SUPFAM; SSF69572; SSF69572; 1.
PE 3: Inferred from homology;
KW Antiviral defense; Reference proteome.
FT CHAIN 1..584
FT /note="CD-NTase-associated protein 2"
FT /id="PRO_0000451857"
SQ SEQUENCE 584 AA; 65493 MW; 8D5BEE2AD4E5B763 CRC64;
MKQELHHTLL GCGFRYTPAK QMPKGILLDT KSRRKGYYVK EYSTKGGVFV IALVLWNDPH
IQLPFAYILQ QPEQYKGRLL PHINFGFCLC YVTQMEADWN SNDLKSTYQD VDEQIQLTLD
NSVASVESGT SNDVELEGEF SAYWQSEEEL YLLAKPSRKA QLKAHLVEAE LSSGSIRREY
VAACSEQSEE LVKWLNQRKF DESSLQEVSI TTHCISVKPN RLAGVNWPPS CLREVLSWLK
LVDYSAHART VTLLMAKRTK RHILLFDVEG QDELAVYLEL NLDVIGKRYF GRNAARKRNI
NNEAALLGGK FVSANFKRLG VTRADRDTLL SRNQSRPDVG NLSQKRIALI GCGTIGGYLA
ELLLRSGAGC GENYFHLYDN DSFKPHNFAR HSLTAHNFGL AKSIALANSL KEAVHIAQSI
KGIDRQFPIQ ADVLSKYDIV IDATGRPPVS KRLAAVARTL IADIRPVLIH AFNDGNGRAS
KVLVDDGRCC YGCMMADPAV YRNNIDLRFE GIDLAKEKHI SCGSTYTPYD AAVSHITAAL
AQEAVLNTLE HTLPWNYSEH MLDGSRSKKP RTLKRFSGCN ICDE
