XMAS_DROME
ID XMAS_DROME Reviewed; 2079 AA.
AC Q9U3V9; A0A4D6K557; C9QP61; D0IQ87; H1UUM5; Q8IR02; Q8MQS3; Q9U3V8; Q9VX76;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 03-AUG-2022, sequence version 4.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Protein xmas {ECO:0000303|PubMed:29779104};
GN Name=xmas {ECO:0000303|PubMed:29779104, ECO:0000312|FlyBase:FBgn0286809};
GN Synonyms=xmas-1 {ECO:0000303|PubMed:29779104,
GN ECO:0000312|FlyBase:FBgn0286809}, xmas-2 {ECO:0000303|PubMed:29779104,
GN ECO:0000312|FlyBase:FBgn0286809};
GN ORFNames=CG46386 {ECO:0000312|FlyBase:FBgn0286809};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
RA Xu E.Y., Kaufman T.C., Wu C.;
RT "Two overlapping genes, xmas-1 and xmas-2, are required for
RT spermatogenesis, oogenesis and embryogenesis.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
RC STRAIN=Berkeley {ECO:0000312|EMBL:ACX36519.1, ECO:0000312|EMBL:ACX85646.1,
RC ECO:0000312|EMBL:AEX98030.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ACX36519.1}, and
RC Head {ECO:0000312|EMBL:ACX85646.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, IDENTIFICATION IN THE AMEX COMPLEX, INTERACTION WITH E(Y)2,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=18034162; DOI=10.1038/sj.emboj.7601901;
RA Kurshakova M.M., Krasnov A.N., Kopytova D.V., Shidlovskii Y.V.,
RA Nikolenko J.V., Nabirochkina E.N., Spehner D., Schultz P., Tora L.,
RA Georgieva S.G.;
RT "SAGA and a novel Drosophila export complex anchor efficient transcription
RT and mRNA export to NPC.";
RL EMBO J. 26:4956-4965(2007).
RN [7]
RP INTERACTION WITH E(Y)2, AND SUBCELLULAR LOCATION.
RX DOI=10.21767/2471-8084.100023;
RA Popova V., Georgieva S., Kopytova D.;
RT "Orc3, A Subunit of Drosophila Pre-Replication Complex Directly Binds mRNA
RT and Interacts with ENY2 Subunit of the TREX-2 mRNA Export Complex.";
RL Biochem. Mol. Biol. J. 2:1-7(2016).
RN [8]
RP FUNCTION, IDENTIFICATION IN THE AMEX COMPLEX, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=27016737; DOI=10.1093/nar/gkw192;
RA Kopytova D., Popova V., Kurshakova M., Shidlovskii Y., Nabirochkina E.,
RA Brechalov A., Georgiev G., Georgieva S.;
RT "ORC interacts with THSC/TREX-2 and its subunits promote Nxf1 association
RT with mRNP and mRNA export in Drosophila.";
RL Nucleic Acids Res. 44:4920-4933(2016).
RN [9]
RP INTERACTION WITH PIWI, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=28472469; DOI=10.1093/nar/gkx355;
RA Ilyin A.A., Ryazansky S.S., Doronin S.A., Olenkina O.M., Mikhaleva E.A.,
RA Yakushev E.Y., Abramov Y.A., Belyakin S.N., Ivankin A.V., Pindyurin A.V.,
RA Gvozdev V.A., Klenov M.S., Shevelyov Y.Y.;
RT "Piwi interacts with chromatin at nuclear pores and promiscuously binds
RT nuclear transcripts in Drosophila ovarian somatic cells.";
RL Nucleic Acids Res. 45:7666-7680(2017).
RN [10]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=29779104; DOI=10.1134/s1607672918020102;
RA Kopytova D.V., Il'in Y.V., Nabirochkina E.N.;
RT "Alternative Splicing of the Xmas mRNA Encoding the mRNA Export Protein in
RT Drosophila melanogaster.";
RL Dokl. Biochem. Biophys. 479:87-89(2018).
RN [11]
RP INTERACTION WITH ORC3, AND MUTAGENESIS OF 1-MET--PRO-252; 1-MET--SER-339;
RP 1-MET--ALA-672; 1-MET--LYS-834 AND 671-ILE--ALA-1359.
RX PubMed=33689068; DOI=10.1134/s1607672921010063;
RA Kurshakova M.M., Kopytova D.V., Georgieva S.G.;
RT "Study of the Interaction between Xmas-2, the Main Protein of TREX-2 mRNA
RT Export Complex, and the Orc3 Protein, a Subunit of ORC Complex of D.
RT melanogaster.";
RL Dokl. Biochem. Biophys. 496:18-21(2021).
RN [12]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=33602059; DOI=10.1080/15476286.2021.1885198;
RA Glukhova A.A., Kurshakova M.M., Nabirochkina E.N., Georgieva S.G.,
RA Kopytova D.V.;
RT "PCID2, a subunit of the Drosophila TREX-2 nuclear export complex, is
RT essential for both mRNA nuclear export and its subsequent cytoplasmic
RT trafficking.";
RL RNA Biol. 18:1969-1980(2021).
CC -!- FUNCTION: Involved in mRNA export and mRNA coupled transcription
CC activation (PubMed:18034162, PubMed:27016737, PubMed:33602059).
CC Component of the nuclear pore complex (NPC)-associated TREX-2/AMEX
CC complex (anchoring and mRNA export complex) which functions in docking
CC export-competent ribonucleoprotein particles (mRNPs) to the nuclear
CC entrance of the nuclear pore complex (nuclear basket), thereby enabling
CC the export of mRNAs to the cytoplasm through the nuclear pores
CC (PubMed:18034162, PubMed:27016737). The TREX-2/AMEX complex also
CC functions with the transcriptional coactivator SAGA/TFTC complex, to
CC anchor a subset of transcription sites to the nuclear pore complex
CC basket in order to achieve efficient transcription and export of their
CC resulting mRNAs (PubMed:18034162). Within the complex, required for
CC localization of e(y)2 to the nuclear periphery (PubMed:18034162).
CC {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737,
CC ECO:0000269|PubMed:33602059}.
CC -!- SUBUNIT: Component of the nuclear pore complex (NPC)-associated TREX-
CC 2/AMEX complex (anchoring and mRNA export complex), composed of e(y)2,
CC xmas and PCID2 (PubMed:18034162, PubMed:27016737). Within the TREX-2/
CC AMEX complex, interactions with e(y)2 is required for localization of
CC e(y)2 to the nuclear periphery (PubMed:18034162, Ref.7). Interaction
CC between the TREX-2/AMEX complex and the ORC complex is required for ORC
CC localization to mRNPs, and consequently mRNA export (PubMed:27016737,
CC PubMed:33689068). Within the TREX-2/AMEX-ORC complex, interacts with
CC Orc6, (via C-terminus) with Orc3, and weakly interacts with Orc4
CC (PubMed:27016737, PubMed:33689068). However, another report found that
CC the interaction with Orc3 is not direct, instead it is mediated via
CC e(y)2 (Ref.7). Interacts with piwi (PubMed:28472469).
CC {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737,
CC ECO:0000269|PubMed:28472469, ECO:0000269|PubMed:33689068,
CC ECO:0000269|Ref.7}.
CC -!- INTERACTION:
CC Q9U3V9; Q9VYX1: e(y)2; NbExp=5; IntAct=EBI-2550689, EBI-2549759;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:28472469}. Nucleus {ECO:0000269|PubMed:29779104,
CC ECO:0000269|Ref.7}. Nucleus membrane {ECO:0000269|PubMed:18034162,
CC ECO:0000269|PubMed:27016737, ECO:0000269|PubMed:28472469,
CC ECO:0000269|PubMed:33602059}; Peripheral membrane protein
CC {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737,
CC ECO:0000269|PubMed:28472469, ECO:0000269|PubMed:33602059}. Cytoplasm
CC {ECO:0000269|PubMed:33602059}. Note=Localizes to nuclear periphery,
CC often in contact with the nuclear pore complex (NPC).
CC {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=C {ECO:0000312|FlyBase:FBgn0286809}; Synonyms=xmas
CC {ECO:0000303|PubMed:29779104};
CC IsoId=Q9U3V9-2; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0286809}; Synonyms=xmas-2
CC {ECO:0000303|PubMed:29779104};
CC IsoId=Q9U3V9-1; Sequence=VSP_061531, VSP_061532;
CC Name=B {ECO:0000312|FlyBase:FBgn0286809}; Synonyms=xmas-1
CC {ECO:0000303|PubMed:29779104};
CC IsoId=Q9U3V9-3; Sequence=VSP_061530;
CC -!- TISSUE SPECIFICITY: Expressed in ovaries (at protein level).
CC {ECO:0000269|PubMed:28472469}.
CC -!- TISSUE SPECIFICITY: [Isoform C]: Detected in the testes and ovaries,
CC with expression levels higher in oocytes than in testicular cells (at
CC protein level). {ECO:0000269|PubMed:29779104}.
CC -!- TISSUE SPECIFICITY: [Isoform A]: Detected in the testes and ovaries (at
CC protein level). {ECO:0000269|PubMed:29779104}.
CC -!- TISSUE SPECIFICITY: [Isoform B]: Detected in the testes.
CC {ECO:0000269|PubMed:29779104}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryos (at protein level).
CC {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737}.
CC -!- DEVELOPMENTAL STAGE: [Isoform C]: Not detected in embryos (at protein
CC level). {ECO:0000269|PubMed:29779104}.
CC -!- DEVELOPMENTAL STAGE: [Isoform A]: Detected in embryos (at protein
CC level). {ECO:0000269|PubMed:29779104}.
CC -!- SIMILARITY: Belongs to the SAC3 family. {ECO:0000305}.
CC -!- CAUTION: Isoform A (xmas-2) and isoform B (xmas-1) were originally
CC thought to be separate genes until a third longer transcript, isoform
CC C, was identified that encompasses both ORFs and supports their
CC existence as three alternatively spliced isoforms (PubMed:29779104).
CC Although most of the functional information for this protein is
CC attributed to isoform A/xmas-2 in the literature, it might also be
CC relevant for isoform C and isoform B/xmas-1.
CC {ECO:0000269|PubMed:29779104, ECO:0000305|PubMed:29779104}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACX36519.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ACX85646.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AEX98030.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF216664; AAF23814.1; -; Genomic_DNA.
DR EMBL; AF216664; AAF23815.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF48702.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09434.1; -; Genomic_DNA.
DR EMBL; AE014298; QCD25243.1; -; Genomic_DNA.
DR EMBL; AY128417; AAM75010.1; -; mRNA.
DR EMBL; BT099943; ACX36519.1; ALT_INIT; mRNA.
DR EMBL; BT100125; ACX85646.1; ALT_INIT; mRNA.
DR EMBL; BT133110; AEX98030.1; ALT_INIT; mRNA.
DR RefSeq; NP_524773.2; NM_080034.3.
DR RefSeq; NP_728054.1; NM_167558.2.
DR AlphaFoldDB; Q9U3V9; -.
DR SMR; Q9U3V9; -.
DR BioGRID; 68935; 20.
DR IntAct; Q9U3V9; 2.
DR MINT; Q9U3V9; -.
DR STRING; 7227.FBpp0074178; -.
DR PaxDb; Q9U3V9; -.
DR PRIDE; Q9U3V9; -.
DR ABCD; Q9U3V9; 2 sequenced antibodies.
DR EnsemblMetazoa; FBtr0474622; FBpp0423199; FBgn0286809.
DR GeneID; 44271; -.
DR KEGG; dme:Dmel_CG46386; -.
DR UCSC; CG32562-RA; d. melanogaster.
DR CTD; 44271; -.
DR FlyBase; FBgn0286809; xmas.
DR VEuPathDB; VectorBase:FBgn0286809; -.
DR eggNOG; KOG1860; Eukaryota.
DR HOGENOM; CLU_003138_0_0_1; -.
DR InParanoid; Q9U3V9; -.
DR OMA; DANFLWD; -.
DR OrthoDB; 1593971at2759; -.
DR PhylomeDB; Q9U3V9; -.
DR BioGRID-ORCS; 44271; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 44271; -.
DR PRO; PR:Q9U3V9; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0286809; Expressed in testis and 22 other tissues.
DR ExpressionAtlas; Q9U3V9; baseline and differential.
DR Genevisible; Q9U3V9; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034399; C:nuclear periphery; IDA:FlyBase.
DR GO; GO:0005643; C:nuclear pore; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0070390; C:transcription export complex 2; IDA:FlyBase.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006406; P:mRNA export from nucleus; IDA:FlyBase.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR CDD; cd12457; RRM_XMAS2; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR045107; SAC3/GANP/THP3.
DR InterPro; IPR005062; SAC3/GANP/THP3_conserved.
DR InterPro; IPR034366; XMAS2_RRM.
DR PANTHER; PTHR12436; PTHR12436; 1.
DR Pfam; PF03399; SAC3_GANP; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50250; PCI; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Membrane; mRNA transport; Nucleus; Oogenesis; Reference proteome;
KW RNA-binding; Spermatogenesis; Transport.
FT CHAIN 1..2079
FT /note="Protein xmas"
FT /id="PRO_0000082006"
FT DOMAIN 12..83
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 342..525
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT REGION 112..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..435
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 689..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 835..1359
FT /note="Sufficient for Orc3 binding"
FT /evidence="ECO:0000269|PubMed:33689068"
FT REGION 1328..1370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1342..1370
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..1360
FT /note="MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMTCT
FT VSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVDPDIQAELSALQSGWR
FT NEYGSGKPIKKPQNGSSGSGGSSMLPAIPVGPATAPVSRDRTPAQLRDLENMMRRPAHT
FT SEEKFSVLDARDKLLRLNRTQHKLSGATQGHCADMCPEKERVLREFQRQVAYYELQPGS
FT DELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMG
FT DWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINA
FT ENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCP
FT EVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSP
FT RKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQINEAGRVVLSRMHTVETEYKLPRQ
FT YELVEVKRVKSVGEVVSGEPLPPRDLYLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQE
FT EMQPQMPSQPPAVSKHSDTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSR
FT AQEFQEQAPATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKLR
FT ERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHKQTRTLELYQE
FT IFQDTLAEICQSEFMSHSRACRSYESMLDSITRDLVERQMEQSIYELGVMRVCIRRWRK
FT YRRTQQEKDTLFNQLPLSFGAENPEGVVNKRSMEDSLRLSRRYRLGEPCDYGKLLAGLE
FT EHSWLKLDLWHVLDKCLPVAQPGARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPD
FT ARFVDGGYIRGFSQGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKP
FT LLQASRCHDVAVIVQHPANTAFVQPDIPLQELCLRSFNIFRLRKSGNNRQRLMIALESA
FT VKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDTAIRSDTQLNPQRCV
FT DLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLPIESSLNTNRLEHFEPGWHLPERR
FT QRIVQLLERCKLPKMPVLPRSSSLAEAQCQWVLDYAQISQQEDCVEQIALQAIKILQYD
FT TDDYLNFVEYLAGERMQYILRQERNPPQGIVYNTKTLKRRFLSAWYYEFREPQIYEPVP
FT AEENAQMLEKQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKR
FT RDASDHKHAM -> MKSLVAQTECQLRPLKV (in isoform B)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_061530"
FT VAR_SEQ 1360..1370
FT /note="MSSLVSALKAA -> SKHKRKRSKSK (in isoform A)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_061531"
FT VAR_SEQ 1371..2079
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_061532"
FT MUTAGEN 1..834
FT /note="Missing: In xmas-2-dCID; no effect on interaction
FT with Orc3."
FT /evidence="ECO:0000269|PubMed:33689068"
FT MUTAGEN 1..672
FT /note="Missing: In xmas-2-C; no effect on interaction with
FT Orc3."
FT /evidence="ECO:0000269|PubMed:33689068"
FT MUTAGEN 1..339
FT /note="Missing: In xmas-2-dM; no effect on interaction with
FT Orc3."
FT /evidence="ECO:0000269|PubMed:33689068"
FT MUTAGEN 1..252
FT /note="Missing: In xmas-2-dRRM; no effect on interaction
FT with Orc3."
FT /evidence="ECO:0000269|PubMed:33689068"
FT MUTAGEN 671..1359
FT /note="Missing: In xmas-2-N; abolishes interaction with
FT Orc3."
FT /evidence="ECO:0000269|PubMed:33689068"
FT CONFLICT 96..281
FT /note="Missing (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="A -> D (in Ref. 5; ACX36519)"
FT /evidence="ECO:0000305"
FT CONFLICT 778
FT /note="S -> N (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 785..787
FT /note="ESM -> VSV (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 942
FT /note="D -> E (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 1027
FT /note="E -> K (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 1217
FT /note="C -> Y (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 1262
FT /note="I -> V (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 1275
FT /note="S -> T (in Ref. 4; AAM75010)"
FT /evidence="ECO:0000305"
FT CONFLICT 1301
FT /note="K -> E (in Ref. 1; AAF23815)"
FT /evidence="ECO:0000305"
FT CONFLICT 1574
FT /note="A -> P (in Ref. 1; AAF23814)"
FT /evidence="ECO:0000305"
FT CONFLICT 1854
FT /note="Missing (in Ref. 5; ACX85646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2079 AA; 239476 MW; BDFE546359936333 CRC64;
MAEPRPGGYN YKTLLCRNIP ELFLDKYVAR SHFGRFGTLV NFVLRPRRMT CTVSYASEDE
AARALLDGAS FQGHLFDISY ADNETAPAQK TEEWVDPDIQ AELSALQSGW RNEYGSGKPI
KKPQNGSSGS GGSSMLPAIP VGPATAPVSR DRTPAQLRDL ENMMRRPAHT SEEKFSVLDA
RDKLLRLNRT QHKLSGATQG HCADMCPEKE RVLREFQRQV AYYELQPGSD ELICHERALK
QYSRSSADQE TPLPHELRNE TALHMTMSYL MHEIMDISER QDPQSHMGDW FHFVWDRTRS
IRKEITQQEL CSLGAVKLVE QCARFHIHCA ARLVDADPSV FDSKINAENL TKCLQTLKYM
YHDLRIKGVP CPKEAEFRGY IVLLNLADAN FLWDIGQLPA ELQSCPEVRQ AIQFYLALQD
TNFVRFFQLL ADKDTSYLSA CILVNYFTRL RVLGLHRLIQ AYRSPRKDEV SSLPLSYIAE
LLSFASEQEA ADFVQHYGLQ INEAGRVVLS RMHTVETEYK LPRQYELVEV KRVKSVGEVV
SGEPLPPRDL YLNHRPHNSF DDYGMLKSIA WTAKDQLAGM QQEEMQPQMP SQPPAVSKHS
DTLFKVPMQP DGTAAGFGVF AAAAVPPASS IDGFSFVLPK SRAQEFQEQA PATQQRRAQE
EAKHQALQVA IAAAKKREAE LMAIHEAKVA EAERVRQQKL RERQEQQRRQ QQELEEQRQR
EQEKLQLEKE RQLKLEQLFF VQQQEREAHK QTRTLELYQE IFQDTLAEIC QSEFMSHSRA
CRSYESMLDS ITRDLVERQM EQSIYELGVM RVCIRRWRKY RRTQQEKDTL FNQLPLSFGA
ENPEGVVNKR SMEDSLRLSR RYRLGEPCDY GKLLAGLEEH SWLKLDLWHV LDKCLPVAQP
GARRFYKLLI SLSGGQEGLQ LNCDLDRGLL QQPQSPDARF VDGGYIRGFS QGIGLSVMKI
RDDDHDWKAT DLAEANGIIC LIGLDDIRLL PDRLKPLLQA SRCHDVAVIV QHPANTAFVQ
PDIPLQELCL RSFNIFRLRK SGNNRQRLMI ALESAVKFLA KATERKRVGH LHQVETREYL
LVNLGSELFR RLKYAAEHDT AIRSDTQLNP QRCVDLFNEA VHRLQLVAGE DLSDWPQFPE
ELRVFVQPLP IESSLNTNRL EHFEPGWHLP ERRQRIVQLL ERCKLPKMPV LPRSSSLAEA
QCQWVLDYAQ ISQQEDCVEQ IALQAIKILQ YDTDDYLNFV EYLAGERMQY ILRQERNPPQ
GIVYNTKTLK RRFLSAWYYE FREPQIYEPV PAEENAQMLE KQPSSQAEVQ QLDFDEITSK
AEAVLKRFHQ RQDERHTLRE LNRSHKSRKR RDASDHKHAM SSLVSALKAA RSDYQGKSSA
VCRLVAQMVE ADQVKYQWTP NLTSLIDRKD ASTRHQWRPH VNHSTRHARK LPVQSISFLS
RCLAKKGEHA GLTSCVLSVK TRYAPKTLHG RELLIWRQSS MRPLLLPSSN LWTGVLSKRH
YVPRLRPAEE DEEVERRAVP QLRITKEQSE EEEHQLQRRK DGSRLRNECS EFYLPMGEQD
YSEREEENRG HRQAYVPSEL MTPELATKWQ TTSVTDRDRQ LLNLEQQMKT QPSVRMTTQT
WFSGNHRRYR SRRSGAKRYH VVSELEGWRR SSNHQPVPVF QGQPGEQEHL RHASHKASAR
SMPDGQERCQ MVWEQKKRSP WHRTEVTNSN KVRMPRIRAA KSAVMMAQEA RNKHHRLITK
KLVYRPRRTV NAVQAEETED QDTHHRHHGG GQKMSKRAPE RALLKQHLAD LLAVSKPEDS
FRIGYQPNAP TRQLLEQGKC YVSLRREQFI HLHPVRPNSL ILAVNLEVKE SPRQPVTTLH
RRDGAKRPRL VEDVVKPSLI TVIRQSAATW DHNGRKVPAK KSNLVGMPSK EHAYQRRLVR
NTSTLEAIVK AQAKPRSEPK SPSSATDYHR VASQKLPHVT SKADISKAAD PFKDLDKPRI
KEKEVASSWK QAYVVRSKMY DAITPYRRRA YPGKKCITKD KTDGYFLAKR SSAAAAGSSK
KVKPPKQPVV SPKVQVPSVL HKKKSRESLG PQTTKTGKL
