CAP4_ARATH
ID CAP4_ARATH Reviewed; 599 AA.
AC Q9SA65; Q1PFZ0;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Putative clathrin assembly protein At1g03050;
GN OrderedLocusNames=At1g03050; ORFNames=F10O3.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
CC -!- SUBCELLULAR LOCATION: Membrane, clathrin-coated pit {ECO:0000250}.
CC Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000250}. Note=Colocalized with clathrin in the Golgi
CC area. {ECO:0000250}.
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DR EMBL; AC006550; AAD25804.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27520.1; -; Genomic_DNA.
DR EMBL; DQ446223; ABE65594.1; -; mRNA.
DR PIR; C86161; C86161.
DR RefSeq; NP_171804.1; NM_100186.3.
DR AlphaFoldDB; Q9SA65; -.
DR SMR; Q9SA65; -.
DR STRING; 3702.AT1G03050.1; -.
DR PaxDb; Q9SA65; -.
DR PRIDE; Q9SA65; -.
DR ProteomicsDB; 240544; -.
DR EnsemblPlants; AT1G03050.1; AT1G03050.1; AT1G03050.
DR GeneID; 839416; -.
DR Gramene; AT1G03050.1; AT1G03050.1; AT1G03050.
DR KEGG; ath:AT1G03050; -.
DR Araport; AT1G03050; -.
DR TAIR; locus:2007524; AT1G03050.
DR eggNOG; KOG0251; Eukaryota.
DR HOGENOM; CLU_014098_3_0_1; -.
DR InParanoid; Q9SA65; -.
DR OMA; TWCKDAY; -.
DR OrthoDB; 532030at2759; -.
DR PhylomeDB; Q9SA65; -.
DR PRO; PR:Q9SA65; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SA65; baseline and differential.
DR Genevisible; Q9SA65; AT.
DR GO; GO:0005905; C:clathrin-coated pit; IBA:GO_Central.
DR GO; GO:0030136; C:clathrin-coated vesicle; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0090404; C:pollen tube tip; IDA:TAIR.
DR GO; GO:0005545; F:1-phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0032050; F:clathrin heavy chain binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0048268; P:clathrin coat assembly; IEA:InterPro.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IBA:GO_Central.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR GO; GO:0072659; P:protein localization to plasma membrane; IGI:TAIR.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR Gene3D; 1.20.58.150; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR011417; ANTH_dom.
DR InterPro; IPR014712; ANTH_dom_sf.
DR InterPro; IPR045192; AP180-like.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR PANTHER; PTHR22951; PTHR22951; 1.
DR Pfam; PF07651; ANTH; 1.
DR SMART; SM00273; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
PE 2: Evidence at transcript level;
KW Coated pit; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane;
KW Reference proteome.
FT CHAIN 1..599
FT /note="Putative clathrin assembly protein At1g03050"
FT /id="PRO_0000187070"
FT DOMAIN 26..162
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT REGION 332..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 580..599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..382
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 599 AA; 66971 MW; B4190E9B926BE1B2 CRC64;
MGSSKFKRAI GAVKDQTSVG LAKVNGRSAS LSELDVAIVK ATRHEEFPAE EKYIREILSL
TSYSRSYINA CVSTLSRRLN KTKCWTVALK TLILIQRLLG EGDQAYEQEI FFATRRGTRL
LNMSDFRDVS RSNSWDYSAF VRTYALYLDE RLDFRMQARH GKRGVYCVGG EADEEEQDQA
AADLSTAIVV RSQPIAEMKT EQIFIRIQHL QQLLDRFLAC RPTGNARNNR VVIVALYPIV
KESFQIYYDV TEIMGILIER FMELDIPDSI KVYDIFCRVS KQFEELDQFY SWCKNMGIAR
SSEYPEIEKI TQKKLDLMDE FIRDKSALEH TKQSKSVKSE ADEDDDEART EEVNEEQEDM
NAIKALPEPP PKEEDDVKPE EEAKEEVIIE KKQEEMGDLL DLGNTNGGEA GQAGDSLALA
LFDGPYASGS GSESGPGWEA FKDDSADWET ALVQTATNLS GQKSELGGGF DMLLLNGMYQ
HGAVNAAVKT STAYGASGSA SSMAFGSAGR PAATMLALPA PSTANGNAGN INSPVPMDPF
AASLEVAPPA YVQMNDMEKK QRMLMEEQMM WDQYSRDGRQ GHMNLRQNQN QPYSYTPQY
