XPF_DICDI
ID XPF_DICDI Reviewed; 964 AA.
AC Q54PN5;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=DNA repair endonuclease XPF;
DE EC=3.1.-.-;
DE AltName: Full=DNA excision repair protein ERCC-4;
GN Name=ercc4; ORFNames=DDB_G0284419;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Structure-specific DNA repair endonuclease responsible for
CC the 5-prime incision during DNA repair. Involved in homologous
CC recombination that assists in removing interstrand cross-link (By
CC similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Heterodimer composed of ercc1 and xpf/ercc4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
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DR EMBL; AAFI02000064; EAL65258.1; -; Genomic_DNA.
DR RefSeq; XP_638619.1; XM_633527.1.
DR AlphaFoldDB; Q54PN5; -.
DR SMR; Q54PN5; -.
DR STRING; 44689.DDB0232364; -.
DR PaxDb; Q54PN5; -.
DR EnsemblProtists; EAL65258; EAL65258; DDB_G0284419.
DR GeneID; 8624590; -.
DR KEGG; ddi:DDB_G0284419; -.
DR dictyBase; DDB_G0284419; xpf.
DR eggNOG; KOG0442; Eukaryota.
DR HOGENOM; CLU_002265_2_0_1; -.
DR InParanoid; Q54PN5; -.
DR OMA; FHKILQA; -.
DR PhylomeDB; Q54PN5; -.
DR Reactome; R-DDI-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR PRO; PR:Q54PN5; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0000110; C:nucleotide-excision repair factor 1 complex; ISS:dictyBase.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0004519; F:endonuclease activity; TAS:dictyBase.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; ISS:dictyBase.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:dictyBase.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:dictyBase.
DR GO; GO:1901255; P:nucleotide-excision repair involved in interstrand cross-link repair; IBA:GO_Central.
DR GO; GO:0006296; P:nucleotide-excision repair, DNA incision, 5'-to lesion; ISS:dictyBase.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR010994; RuvA_2-like.
DR Pfam; PF02732; ERCC4; 1.
DR SMART; SM00891; ERCC4; 1.
DR SUPFAM; SSF47781; SSF47781; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..964
FT /note="DNA repair endonuclease XPF"
FT /id="PRO_0000331368"
FT DOMAIN 739..819
FT /note="ERCC4"
FT REGION 360..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 361..379
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..496
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..523
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 964 AA; 110646 MW; DF43358D74CEF98B CRC64;
MSLEFYKQIF EDCLNDDGLV VMGSGLGIHN IILGFLKFYS DTDDLVLFLD CQSNESLQNS
YLFYHERLLN FGIKYSNLPT MVNVETVSSS TKTNMYSKGG VYFGASSIFV LDYLTKRMPC
NLVSGIIIQN AHRITDTSIE YLLIKLFRQN NKKGFIKAFT TEPSLLVDEI GKLQRTMKYL
HLKKLFLWPR FHQHISLILD KHPPDLVELS IGMTNSMKRI EESLHLNTQR CISSLIAINK
LPRLNGGNGG NDSDSFEQIL KQQLKPMWSK LNLHSKQLVS SIKLLNQLKN NLLVYDCVTF
YSLLLYIQAS SDSLKEGLHT NSNYVDEVQP WLESKEAQNL FSASQERVYR FKKLNSLKSP
PKKLKTINND SSSSSSSSNL EKILTLEDNP KWNVLYQILQ EIEEDKEKQG TILIFVKDET
TVNQLSTYLD YGGYSFLLGK YEKLCSNQQY LDELRKKKEE NNNNNNSNNN NNNNNNNNNN
NSVKSLSSND NGGYSRYRRT KNFKDNRKQQ IIETSKKKKG KGGTNDQNLF NMGIQLVDGH
NKLNIPNPKF INQDGSGGGG DNGDENNNNN NNLNINDGLT IASMNEEING LNDFYELLSP
PYIVIHPISK SLTILDEIRP TFIIVYDPDI SITRQIEVYK AENPGTPVRL YFMTYSDSSE
EYQYISKLQR EKSSFEKLIR EKTNLIIDTE QEGKIQLVDN SKLELLDDMK STRNSRLGGL
MKNFDSIQQQ QQQQQQKKTI IIDSHEFKSS LPVVLHNNGY EIIPLRLEVG DFVLSPIHCI
ERKSVSDLIG SFNSGRLFTQ IEAMNRIYKN PILLIEFDPN QPFYLVAPDE LQKDYLSPFS
LPSKLVFLTK TFPRLRVIWS RSYYCTTKIY DQIKDGYPEP DPSMVNVIPE VNDDQNYNFN
AQDVLRTMPG VNDKNINLIM DNVDDLYHLS KLSLSEFSTI MNDSVNNNKN ATLLFNFFNN
QLNQ
