XPO1A_ARATH
ID XPO1A_ARATH Reviewed; 1075 AA.
AC Q9SMV6; F4KFL2;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 151.
DE RecName: Full=Protein EXPORTIN 1A {ECO:0000303|PubMed:18791220};
DE Short=AtCRM1 {ECO:0000303|PubMed:10652141};
DE Short=AtXPO1 {ECO:0000303|PubMed:10652141};
DE AltName: Full=Protein HEAT-INTOLERANT 2 {ECO:0000303|PubMed:20345641};
GN Name=XPO1 {ECO:0000303|PubMed:10652141};
GN Synonyms=HIT2 {ECO:0000303|PubMed:20345641},
GN XPO1A {ECO:0000303|PubMed:18791220};
GN OrderedLocusNames=At5g17020 {ECO:0000312|Araport:AT5G17020};
GN ORFNames=F2K13.170 {ECO:0000312|EMBL:CAC01715.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:CAB56597.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, INTERACTION WITH RAN1,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10652141; DOI=10.1046/j.1365-313x.1999.00644.x;
RA Haasen D., Koehler C., Neuhaus G., Merkle T.;
RT "Nuclear export of proteins in plants: AtXPO1 is the export receptor for
RT leucine-rich nuclear export signals in Arabidopsis thaliana.";
RL Plant J. 20:695-705(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia; TISSUE=Leaf, and Stem;
RA Haasen D., Neuhaus G., Merkle T.;
RT "Isolation and sequence analysis of a genomic clone of the nuclear export
RT receptor AtXPO1 (AtCRM1) from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR99-127(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION.
RX PubMed=16766674; DOI=10.1104/pp.106.081406;
RA Jakoby M.J., Weinl C., Pusch S., Kuijt S.J.H., Merkle T., Dissmeyer N.,
RA Schnittger A.;
RT "Analysis of the subcellular localization, function, and proteolytic
RT control of the Arabidopsis cyclin-dependent kinase inhibitor ICK1/KRP1.";
RL Plant Physiol. 141:1293-1305(2006).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=18791220; DOI=10.1534/genetics.108.094896;
RA Blanvillain R., Boavida L.C., McCormick S., Ow D.W.;
RT "Exportin1 genes are essential for development and function of the
RT gametophytes in Arabidopsis thaliana.";
RL Genetics 180:1493-1500(2008).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=20345641; DOI=10.1111/j.1469-8137.2010.03225.x;
RA Wu S.-J., Wang L.-C., Yeh C.-H., Lu C.-A., Wu S.-J.;
RT "Isolation and characterization of the Arabidopsis heat-intolerant 2 (hit2)
RT mutant reveal the essential role of the nuclear export receptor EXPORTIN1A
RT (XPO1A) in plant heat tolerance.";
RL New Phytol. 186:833-842(2010).
CC -!- FUNCTION: Receptor for the leucine-rich nuclear export signal (NES).
CC Binds cooperatively to the NES on its target protein and to the small
CC GTPase Ran in its active GTP-bound form (PubMed:10652141,
CC PubMed:16766674). Required for the maternal-to-embryonic transition and
CC during gametophyte development (PubMed:18791220). Involved in heat-
CC induced oxidative stress basal resistance (PubMed:20345641).
CC {ECO:0000269|PubMed:10652141, ECO:0000269|PubMed:16766674,
CC ECO:0000269|PubMed:18791220, ECO:0000269|PubMed:20345641}.
CC -!- SUBUNIT: Interacts with RAN1. {ECO:0000269|PubMed:10652141}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:Q9TVM2}. Nucleus membrane
CC {ECO:0000250|UniProtKB:Q9TVM2}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q9TVM2}; Nucleoplasmic side
CC {ECO:0000250|UniProtKB:Q9TVM2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SMV6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SMV6-2; Sequence=VSP_057489;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously, with higher levels in
CC stems, inflorescences and roots (PubMed:10652141, PubMed:20345641).
CC Present in mature pollen grains, unpollinated pistils, and 2-week-old
CC seedlings (PubMed:18791220). {ECO:0000269|PubMed:10652141,
CC ECO:0000269|PubMed:18791220, ECO:0000269|PubMed:20345641}.
CC -!- DISRUPTION PHENOTYPE: Gametophyte defective when both XPO1A and XPO1B
CC are disrupted. Abnormal pollen germination and tube growth, impaired
CC female gametophyte development and embryo lethal (PubMed:18791220).
CC Impaired ability to withstand moderate heat stress (37 degrees
CC Celsius). Enhanced sensitivity to methyl viologen (MV)-induced
CC oxidative stress (PubMed:20345641). {ECO:0000269|PubMed:18791220,
CC ECO:0000269|PubMed:20345641}.
CC -!- SIMILARITY: Belongs to the exportin family. {ECO:0000305}.
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DR EMBL; Y18469; CAB89280.1; -; mRNA.
DR EMBL; Y18470; CAB56597.1; -; Genomic_DNA.
DR EMBL; AL391141; CAC01715.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92373.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92374.1; -; Genomic_DNA.
DR EMBL; AY056126; AAL07205.1; -; mRNA.
DR EMBL; AY096633; AAM20283.1; -; mRNA.
DR PIR; T51557; T51557.
DR PIR; T52638; T52638.
DR RefSeq; NP_001190324.1; NM_001203395.1. [Q9SMV6-2]
DR RefSeq; NP_197204.1; NM_121708.3. [Q9SMV6-1]
DR AlphaFoldDB; Q9SMV6; -.
DR SMR; Q9SMV6; -.
DR IntAct; Q9SMV6; 2.
DR STRING; 3702.AT5G17020.1; -.
DR iPTMnet; Q9SMV6; -.
DR PaxDb; Q9SMV6; -.
DR PRIDE; Q9SMV6; -.
DR ProMEX; Q9SMV6; -.
DR ProteomicsDB; 242512; -. [Q9SMV6-1]
DR EnsemblPlants; AT5G17020.1; AT5G17020.1; AT5G17020. [Q9SMV6-1]
DR EnsemblPlants; AT5G17020.2; AT5G17020.2; AT5G17020. [Q9SMV6-2]
DR GeneID; 831565; -.
DR Gramene; AT5G17020.1; AT5G17020.1; AT5G17020. [Q9SMV6-1]
DR Gramene; AT5G17020.2; AT5G17020.2; AT5G17020. [Q9SMV6-2]
DR KEGG; ath:AT5G17020; -.
DR Araport; AT5G17020; -.
DR TAIR; locus:2148216; AT5G17020.
DR eggNOG; KOG2020; Eukaryota.
DR HOGENOM; CLU_011906_0_0_1; -.
DR InParanoid; Q9SMV6; -.
DR OMA; HNINTLC; -.
DR PhylomeDB; Q9SMV6; -.
DR PRO; PR:Q9SMV6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SMV6; baseline and differential.
DR Genevisible; Q9SMV6; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005049; F:nuclear export signal receptor activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0009553; P:embryo sac development; IGI:TAIR.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009846; P:pollen germination; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR GO; GO:0006611; P:protein export from nucleus; ISS:TAIR.
DR GO; GO:0009408; P:response to heat; IMP:UniProtKB.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IBA:GO_Central.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR041123; CRM1_repeat.
DR InterPro; IPR041235; Exp1_repeat_2.
DR InterPro; IPR013598; Exportin-1/Importin-b-like.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR045065; XPO1/5.
DR InterPro; IPR014877; XPO1_C_dom.
DR InterPro; IPR040485; XPO1_repeat_3.
DR PANTHER; PTHR11223; PTHR11223; 1.
DR Pfam; PF08767; CRM1_C; 1.
DR Pfam; PF18777; CRM1_repeat; 1.
DR Pfam; PF18784; CRM1_repeat_2; 1.
DR Pfam; PF18787; CRM1_repeat_3; 1.
DR Pfam; PF03810; IBN_N; 1.
DR Pfam; PF08389; Xpo1; 1.
DR SMART; SM01102; CRM1_C; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Membrane; mRNA transport;
KW Nuclear pore complex; Nucleus; Protein transport; Reference proteome;
KW Repeat; Translocation; Transport.
FT CHAIN 1..1075
FT /note="Protein EXPORTIN 1A"
FT /id="PRO_0000432145"
FT DOMAIN 37..103
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 91..130
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 135..171
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 232..267
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 336..373
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 388..425
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 474..513
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 563..600
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 612..649
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 682..719
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 756..793
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 798..835
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 894..934
FT /note="HEAT 12"
FT /evidence="ECO:0000255"
FT VAR_SEQ 308..322
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_057489"
SQ SEQUENCE 1075 AA; 123243 MW; 9FAEC2F8421848B8 CRC64;
MAAEKLRDLS QPIDVGVLDA TVAAFFVTGS KEERAAADQI LRDLQANPDM WLQVVHILQN
TNSLDTKFFA LQVLEGVIKY RWNALPVEQR DGMKNYISEV IVQLSSNEAS FRSERLYVNK
LNVILVQIVK HDWPAKWTSF IPDLVAAAKT SETICENCMA ILKLLSEEVF DFSRGEMTQQ
KIKELKQSLN SEFKLIHELC LYVLSASQRQ DLIRATLSAL HAYLSWIPLG YIFESTLLET
LLKFFPVPAY RNLTIQCLTE VAALNFGDFY NVQYVKMYTI FIGQLRIILP PSTKIPEAYS
SGSGEEQAFI QNLALFFTSF FKFHIRVLES TPEVVSLLLA GLEYLINISY VDDTEVFKVC
LDYWNSLVLE LFDAHHNSDN PAVSASLMGL QPFLPGMVDG LGSQVMQRRQ LYSHPMSKLR
GLMINRMAKP EEVLIVEDEN GNIVRETMKD NDVLVQYKIM RETLIYLSHL DHDDTEKQML
RKLNKQLSGE EWAWNNLNTL CWAIGSISGS MAEDQENRFL VMVIRDLLNL CEITKGKDNK
AVIASNIMYV VGQYPRFLRA HWKFLKTVVN KLFEFMHETH PGVQDMACDT FLKIVQKCKR
KFVIVQVGEN EPFVSELLTG LATTVQDLEP HQIHSFYESV GNMIQAESDP QKRDEYLQRL
MALPNQKWAE IIGQARHSVE FLKDQVVIRT VLNILQTNTS AATSLGTYFL SQISLIFLDM
LNVYRMYSEL VSTNITEGGP YASKTSFVKL LRSVKRETLK LIETFLDKAE DQPHIGKQFV
PPMMESVLGD YARNVPDARE SEVLSLFATI INKYKATMLD DVPHIFEAVF QCTLEMITKN
FEDYPEHRLK FFSLLRAIAT FCFPALIKLS SPQLKLVMDS IIWAFRHTER NIAETGLNLL
LEMLKNFQQS EFCNQFYRSY FMQIEQEIFA VLTDTFHKPG FKLHVLVLQQ LFCLPESGAL
TEPLWDATTV PYPYPDNVAF VREYTIKLLS SSFPNMTAAE VTQFVNGLYE SRNDPSGFKN
NIRDFLVQSK EFSAQDNKDL YAEEAAAQRE RERQRMLSIP GLIAPNEIQD EMVDS
