ID   CAP8A_STAAU             Reviewed;         222 AA.
AC   P72367;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Capsular polysaccharide type 8 biosynthesis protein cap8A;
GN   Name=cap8A;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Becker;
RX   PubMed=8606192; DOI=10.1128/jb.178.7.2118-2126.1996;
RA   Sau S., Lee C.Y.;
RT   "Cloning of type 8 capsule genes and analysis of gene clusters for the
RT   production of different capsular polysaccharides in Staphylococcus
RT   aureus.";
RL   J. Bacteriol. 178:2118-2126(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Becker;
RX   PubMed=9045821; DOI=10.1128/jb.179.5.1614-1621.1997;
RA   Sau S., Sun J., Lee C.Y.;
RT   "Molecular characterization and transcriptional analysis of type 8 capsule
RT   genes in Staphylococcus aureus.";
RL   J. Bacteriol. 179:1614-1621(1997).
RN   [3]
RP   FUNCTION.
RC   STRAIN=Becker;
RX   PubMed=9245821; DOI=10.1099/00221287-143-7-2395;
RA   Sau S., Bhasin N., Wann E.R., Lee J.C., Foster T.J., Lee C.Y.;
RT   "The Staphylococcus aureus allelic genetic loci for serotype 5 and 8
RT   capsule expression contain the type-specific genes flanked by common
RT   genes.";
RL   Microbiology 143:2395-2405(1997).
CC   -!- FUNCTION: Required for the biosynthesis of type 8 capsular
CC       polysaccharide (Cap8/CP8). Might act as the chain-length regulator.
CC       {ECO:0000269|PubMed:9245821}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CpsC/CapA family. {ECO:0000305}.
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DR   EMBL; U73374; AAB49430.1; -; Genomic_DNA.
DR   PDB; 3BFV; X-ray; 1.80 A; A/B=194-222.
DR   PDBsum; 3BFV; -.
DR   AlphaFoldDB; P72367; -.
DR   SMR; P72367; -.
DR   TCDB; 8.A.3.2.4; the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.
DR   EvolutionaryTrace; P72367; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR   GO; GO:0015774; P:polysaccharide transport; IEA:InterPro.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   InterPro; IPR005701; MPA1-like.
DR   Pfam; PF02706; Wzz; 1.
DR   TIGRFAMs; TIGR01006; polys_exp_MPA1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Capsule biogenesis/degradation; Cell membrane;
KW   Exopolysaccharide synthesis; Membrane; Transmembrane; Transmembrane helix;
KW   Virulence.
FT   CHAIN           1..222
FT                   /note="Capsular polysaccharide type 8 biosynthesis protein
FT                   cap8A"
FT                   /id="PRO_0000217225"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   222 AA;  24867 MW;  260072CA71E1164F CRC64;
     MESTLELTKI KEVLQKNLKI LIILPLLFLI ISAIVTFFVL SPKYQANTQI LVNQTKGDNP
     QFMAQEVQSN IQLVNTYKEI VKSPRILDEV SKDLNNKYSP SKLSSMLTIT NQENTQLINI
     QVKSGHKQDS EKIANSFAKV TSKQIPKIMS LDNVSILSKA DGTAVKVAPK TVVNLIGAFF
     LGLVVALIYI FFKVIFDKRI KDEEDVEKEL GLPVLGSIQK FN