XRCC4_ARATH
ID XRCC4_ARATH Reviewed; 264 AA.
AC Q682V0; Q9LS81;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=DNA repair protein XRCC4;
GN Name=XRCC4; OrderedLocusNames=At3g23100; ORFNames=MXC7.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND INTERACTION WITH LIG4.
RC STRAIN=cv. Columbia;
RX PubMed=11029705; DOI=10.1046/j.1365-313x.2000.00856.x;
RA West C.E., Waterworth W.M., Jiang Q., Bray C.M.;
RT "Arabidopsis DNA ligase IV is induced by gamma-irradiation and interacts
RT with an Arabidopsis homologue of the double strand break repair protein
RT XRCC4.";
RL Plant J. 24:67-78(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-264.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-264.
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH POLL.
RX PubMed=23660835; DOI=10.1104/pp.113.219022;
RA Roy S., Choudhury S.R., Sengupta D.N., Das K.P.;
RT "Involvement of AtPollambda in the repair of high salt- and DNA cross-
RT linking agent-induced double strand breaks in Arabidopsis.";
RL Plant Physiol. 162:1195-1210(2013).
CC -!- FUNCTION: May be involved in DNA non-homologous end joining (NHEJ)
CC required for double-strand break repair. May bind to DNA. The LIG4-
CC XRCC4 complex is probably responsible for the NHEJ ligation step, and
CC XRCC4 may enhance the joining activity of LIG4 (By similarity).
CC {ECO:0000250|UniProtKB:Q13426}.
CC -!- SUBUNIT: Interacts with LIG4 and specifically binds its tandem BRCT
CC domains (PubMed:11029705). Interacts with POLL (PubMed:23660835).
CC {ECO:0000269|PubMed:11029705, ECO:0000269|PubMed:23660835}.
CC -!- INTERACTION:
CC Q682V0; Q9LL84: LIG4; NbExp=2; IntAct=EBI-2128002, EBI-2127971;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Slightly induced by gamma radiation, but not by white light
CC or by UV-B. {ECO:0000269|PubMed:11029705}.
CC -!- SIMILARITY: Belongs to the XRCC4-XLF family. XRCC4 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAV91340.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAX49370.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD43030.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF233528; AAF91285.2; -; mRNA.
DR EMBL; AB026655; BAB02102.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76717.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76718.1; -; Genomic_DNA.
DR EMBL; AK175267; BAD43030.1; ALT_INIT; mRNA.
DR EMBL; BT020394; AAV91340.1; ALT_INIT; mRNA.
DR EMBL; BT021921; AAX49370.1; ALT_INIT; mRNA.
DR RefSeq; NP_001154639.1; NM_001161167.1.
DR RefSeq; NP_188951.2; NM_113211.2.
DR AlphaFoldDB; Q682V0; -.
DR SMR; Q682V0; -.
DR BioGRID; 7217; 3.
DR IntAct; Q682V0; 1.
DR STRING; 3702.AT3G23100.2; -.
DR iPTMnet; Q682V0; -.
DR PaxDb; Q682V0; -.
DR PRIDE; Q682V0; -.
DR ProteomicsDB; 242795; -.
DR EnsemblPlants; AT3G23100.1; AT3G23100.1; AT3G23100.
DR EnsemblPlants; AT3G23100.2; AT3G23100.2; AT3G23100.
DR GeneID; 821885; -.
DR Gramene; AT3G23100.1; AT3G23100.1; AT3G23100.
DR Gramene; AT3G23100.2; AT3G23100.2; AT3G23100.
DR KEGG; ath:AT3G23100; -.
DR Araport; AT3G23100; -.
DR TAIR; locus:2094593; AT3G23100.
DR eggNOG; ENOG502QWJA; Eukaryota.
DR HOGENOM; CLU_063979_0_0_1; -.
DR InParanoid; Q682V0; -.
DR OMA; MQKDISF; -.
DR OrthoDB; 940052at2759; -.
DR PhylomeDB; Q682V0; -.
DR PRO; PR:Q682V0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q682V0; baseline and differential.
DR Genevisible; Q682V0; AT.
DR GO; GO:0032807; C:DNA ligase IV complex; IBA:GO_Central.
DR GO; GO:0005958; C:DNA-dependent protein kinase-DNA ligase 4 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:TAIR.
DR GO; GO:0051103; P:DNA ligation involved in DNA repair; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0051351; P:positive regulation of ligase activity; IBA:GO_Central.
DR GO; GO:0010165; P:response to X-ray; IBA:GO_Central.
DR Gene3D; 1.20.5.370; -; 1.
DR Gene3D; 2.170.210.10; -; 1.
DR InterPro; IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
DR InterPro; IPR010585; DNA_repair_prot_XRCC4.
DR InterPro; IPR014751; XRCC4-like_C.
DR InterPro; IPR038051; XRCC4-like_N_sf.
DR InterPro; IPR009089; XRCC4_N_sf.
DR PANTHER; PTHR28559; PTHR28559; 1.
DR Pfam; PF06632; XRCC4; 1.
DR SUPFAM; SSF50809; SSF50809; 1.
DR PROSITE; PS00867; CPSASE_2; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..264
FT /note="DNA repair protein XRCC4"
FT /id="PRO_0000066049"
FT REGION 213..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..252
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 264 AA; 29854 MW; ACDFCCB198367F25 CRC64;
MIGVDSKSSS TTFIETMVES EKTKHTCLRL EISGADPIFV KGTWHNSRFD ISVTDGSSSW
ICNATEEEVA ERAAQWDQPV SEYLKLAEQY LGFQQPNSVY SFSDALEGSK RLSWTFEKEG
TKLEWRWKCK PSDDSKKITV GILDFLMEAN IRLSEEVVNK TRSFEKMRSE AERCLAQGEK
LCDEKTEFES ATYAKFLSVL NAKKAKLRAL RDKEDSVRVV EEEESTDKAE SFESGRSDDE
KSEEEASKKA TSSKARGGKR AARS
