XRN2_ASHGO
ID XRN2_ASHGO Reviewed; 945 AA.
AC Q74ZA0;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 4.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=5'-3' exoribonuclease 2;
DE EC=3.1.13.-;
GN Name=RAT1; OrderedLocusNames=AGR303W;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 311; 790-816; 833 AND 875.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. Required for the
CC processing of nuclear mRNA and rRNA precursors. May promote termination
CC of transcription by RNA polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC subfamily. {ECO:0000305}.
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DR EMBL; AE016820; AAS54793.2; -; Genomic_DNA.
DR RefSeq; NP_986969.2; NM_212031.2.
DR AlphaFoldDB; Q74ZA0; -.
DR SMR; Q74ZA0; -.
DR STRING; 33169.AAS54793; -.
DR PRIDE; Q74ZA0; -.
DR EnsemblFungi; AAS54793; AAS54793; AGOS_AGR303W.
DR GeneID; 4623272; -.
DR KEGG; ago:AGOS_AGR303W; -.
DR eggNOG; KOG2044; Eukaryota.
DR HOGENOM; CLU_006038_1_1_1; -.
DR OMA; CLHYYVH; -.
DR Proteomes; UP000000591; Chromosome VII.
DR GO; GO:0090730; C:Las1 complex; IEA:EnsemblFungi.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0110103; C:RNA polymerase II termination complex; IEA:EnsemblFungi.
DR GO; GO:0004534; F:5'-3' exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:EnsemblFungi.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1901408; P:negative regulation of phosphorylation of RNA polymerase II C-terminal domain; IEA:EnsemblFungi.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IEA:EnsemblFungi.
DR GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IEA:EnsemblFungi.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IBA:GO_Central.
DR GO; GO:1904595; P:positive regulation of termination of RNA polymerase II transcription; IEA:EnsemblFungi.
DR GO; GO:0043144; P:sno(s)RNA processing; IEA:EnsemblFungi.
DR GO; GO:0030847; P:termination of RNA polymerase II transcription, exosome-dependent; IEA:EnsemblFungi.
DR GO; GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IEA:EnsemblFungi.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR004859; Put_53exo.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR017151; Xrn2/3/4.
DR PANTHER; PTHR12341; PTHR12341; 1.
DR Pfam; PF17846; XRN_M; 1.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF037239; Exonuclease_Xrn2; 1.
PE 3: Inferred from homology;
KW Exonuclease; Hydrolase; mRNA processing; Nuclease; Nucleus;
KW Reference proteome; rRNA processing; Transcription;
KW Transcription regulation; Transcription termination.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..945
FT /note="5'-3' exoribonuclease 2"
FT /id="PRO_0000249917"
FT REGION 473..510
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 524..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 912..945
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..504
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 549..564
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 945 AA; 107269 MW; C778BA967A02C49F CRC64;
MGVPSFFRWL SRKYPKIISP VIEDTPQVVD GVKLPIDYSA ANPNGELDNL YLDMNGIVHP
CSHPENKPAP ETEDEMLLAV FEYTNRVLNM ARPRKVLMIA VDGVAPRAKM NQQRSRRFRS
ARDAKLANEE KARVLAEREA YGEMIDDAVK AKKSWDTNAI TPGTPFMDKL AAALRYWTSF
KLATDPGWKN LQVIISDATV PGEGEHKIMN FIRSQRADVQ YNPNTTHCIY GLDADLIFLG
LATHEPHFKI LREDVFAQDN RRRPRQHDMM DMSAEEKQAL IEQDAEKPFL WLHVSVLREY
LSAELLVPRL PFHFDLERAI DDWVFMCFFC GNDFLPHLPS LDVRENSIDI LVDIWKAVLP
SLKTYLTCDG KLNLKGVEKV LAQLGNRESD IFKKKYEQEL RKKEAQERRK MLKNNPNVTK
GKVDRNFTVP LESMPVFDVN GNAAAGSLNL TNKDFANMRK EITLANEGNT KALDALKRRS
DENRTQRTSD TRDFSQEELE SAIEVSKGDN LSAAASLRER ILAKRKAAED PEGAPEPKNP
HKAPSASETE AATPASSETA SQVDIPISTG MLTPGFLDTD TGVRLFEPGY HDRYYQAKFH
VPTGSIDALR KKVVRSYVEG ISWVLLYYYQ GCPSWTWYYP YHYAPFASDF TGISDLSVTF
DLGEPFLPYE QLMSVLPAAS SHNLPDVFRS LMSDPESEIY DFYPEEFPID MNGEKMAWKA
LVLLPFIDET RLLAAVRQKY PLLSEAERAR NVRRDEVLLI SNKNTHYERF LARLYTDPAP
QPVNFRHFKS GLAGTALSDA EGFRPNTKML CPLAAGSLPD ISTNLFLKTA YRMPQLPAPS
KSILLNGFIP PEAQLSPADR DAIMYKYPQR WNPHTMKHNL VPVGPAAVTQ YQPRIGGYRA
FLFHQQLMQD RPYPPAHMHS HSSGGPPLAQ GPRVTQSRYQ PRRFH
