XRN2_CAEBR
ID XRN2_CAEBR Reviewed; 976 AA.
AC Q60SG7; A8XYW7;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=5'-3' exoribonuclease 2 homolog;
DE EC=3.1.13.-;
GN Name=xrn-2 {ECO:0000312|WormBase:CBG20900};
GN ORFNames=CBG20900 {ECO:0000312|WormBase:CBG20900};
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. Plays a role in
CC maintenance of steady-state concentration and turnover of microRNAs
CC (miRNA) by degradation of mature miRNA. Degradation role is enhanced
CC when in complex with paxt-1. Partially redundant to xrn-1 in miRNA
CC guide strand degradation. Implicated in differential regulation of
CC mRNAs such as let-7 by controlling the accumulation of mature miRNA.
CC Positively regulates molting of the pharyngeal cuticle.
CC {ECO:0000250|UniProtKB:Q9U299}.
CC -!- SUBUNIT: Interacts with paxt-1 (via N-terminus); the interaction is
CC direct and results in stabilization of xrn-2 in the complex.
CC {ECO:0000250|UniProtKB:Q9U299}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9U299}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC subfamily. {ECO:0000305}.
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DR EMBL; HE600928; CAP37834.2; -; Genomic_DNA.
DR AlphaFoldDB; Q60SG7; -.
DR SMR; Q60SG7; -.
DR STRING; 6238.CBG20900; -.
DR PRIDE; Q60SG7; -.
DR EnsemblMetazoa; CBG20900.1; CBG20900.1; WBGene00039805.
DR WormBase; CBG20900; CBP30614; WBGene00039805; Cbr-xrn-2.
DR eggNOG; KOG2044; Eukaryota.
DR HOGENOM; CLU_006038_1_2_1; -.
DR InParanoid; Q60SG7; -.
DR OMA; CLHYYVH; -.
DR OrthoDB; 685597at2759; -.
DR Proteomes; UP000008549; Chromosome II.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008409; F:5'-3' exonuclease activity; ISS:UniProtKB.
DR GO; GO:0004534; F:5'-3' exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000738; P:DNA catabolic process, exonucleolytic; ISS:UniProtKB.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR GO; GO:0010587; P:miRNA catabolic process; IEA:EnsemblMetazoa.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0060965; P:negative regulation of miRNA-mediated gene silencing; IEA:EnsemblMetazoa.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IBA:GO_Central.
DR GO; GO:0040034; P:regulation of development, heterochronic; IEA:EnsemblMetazoa.
DR GO; GO:0040028; P:regulation of vulval development; IEA:EnsemblMetazoa.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR004859; Put_53exo.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR017151; Xrn2/3/4.
DR PANTHER; PTHR12341; PTHR12341; 1.
DR Pfam; PF17846; XRN_M; 2.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF037239; Exonuclease_Xrn2; 1.
PE 3: Inferred from homology;
KW Exonuclease; Hydrolase; Metal-binding; mRNA processing; Nuclease; Nucleus;
KW Reference proteome; Transcription; Transcription regulation;
KW Transcription termination; Zinc; Zinc-finger.
FT CHAIN 1..976
FT /note="5'-3' exoribonuclease 2 homolog"
FT /id="PRO_0000249915"
FT ZN_FING 264..281
FT /note="CCHC-type"
FT REGION 411..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 535..788
FT /note="Interaction with paxt-1"
FT /evidence="ECO:0000250|UniProtKB:Q9U299"
FT REGION 815..976
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 976 AA; 111453 MW; BCF42CBA1961DE28 CRC64;
MGVPAFFRWL TKKYPATVVN ANEDRQRGVD GRRVPVDCTQ PNPNFQEFDN LYLDMNGIIH
PCTHPEDRPA PKNEDEMFAL IFEYIDRIFS IVRPRRLLYM AIDGVAPRAK MNQQRSRRFR
ASKEMAEKAA SIEEQRRRLI AEGIAVPQKK KDEEEAHFDS NCITPGTPFM ARLADALRYY
IHDRVTNDPA WANIEIILSD ANVPGEGEHK IMDYIRKQRG NPAHDPNTVH CLCGADADLI
MLGIATHEAN FNIIREEFVP NQPRACELCG QYGHELKECR GAENDTDLGD EYCKPEQREK
NFIFLRIPVL REYLEKEMAM PNLPFQFNLE RALDDWVFLC FFVGNDFLPH LPSLEIREGA
IDRLIKLYKE MVYEMKGYLT KDGIPELDRV EMIMRGLGKV EDEIFKRRQQ DEERFKENQK
NKKARMQQYG RGRGGRGRGR GQPAYVPSHG ILAPMSAPMH HSGESTRQMA SDARQAAMQF
NATNDANAQA AANLKALLNV KGEQSPAEVA AQESRKRKAE QPIIITDEDE EPKDDIRLYE
SGWKERYYRA KFDVGSDDVD FRHRVAWAYV EGLCWVLRYY YQGCSSWDWY FPYHYAPFAS
DFETVGEFKP DFTRPTKPFN PLEQLMSVFP AASKQHLPVE WQKLMTEDES PIIDLYPADF
RIDLNGKKYA WQGVALLPFV DEQRLLETLK SVYPTLTDEE KYRNTRGPNR IFIGRNHKSF
AFFQQVAESK SNDLVDLDPS LLNGVSGKIS YDSTATAPGL PFPSPVSHEE CQDLPTNCGI
CVLYEDPEYP ANYVFPAVRL DGAKEAEKTL RPEDWNERRD GRFNPTIGFN RNAPRGGLDL
SGQRHINHHV RGAMYDRQGG NDNYRGGYRG GYQGGYDDRR GGRGGGGYRG GYNDSRPDFG
RNYAGREGGG PQHYHEHQPG GGHGRPHDQQ PYQDNRRGGG YHRGGRGNGP TGYQRPPYRG
GRGRGGGGYQ GNSSWR
