XRN2_KLULA
ID XRN2_KLULA Reviewed; 992 AA.
AC Q6CKX0;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=5'-3' exoribonuclease 2;
DE EC=3.1.13.-;
GN Name=RAT1; OrderedLocusNames=KLLA0F07469g;
OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=284590;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. Required for the
CC processing of nuclear mRNA and rRNA precursors. May promote termination
CC of transcription by RNA polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR382126; CAG98127.1; -; Genomic_DNA.
DR RefSeq; XP_455419.1; XM_455419.1.
DR AlphaFoldDB; Q6CKX0; -.
DR SMR; Q6CKX0; -.
DR STRING; 28985.XP_455419.1; -.
DR EnsemblFungi; CAG98127; CAG98127; KLLA0_F07469g.
DR GeneID; 2895935; -.
DR KEGG; kla:KLLA0_F07469g; -.
DR eggNOG; KOG2044; Eukaryota.
DR HOGENOM; CLU_006038_1_1_1; -.
DR InParanoid; Q6CKX0; -.
DR OMA; CLHYYVH; -.
DR Proteomes; UP000000598; Chromosome F.
DR GO; GO:0090730; C:Las1 complex; IEA:EnsemblFungi.
DR GO; GO:0110103; C:RNA polymerase II termination complex; IEA:EnsemblFungi.
DR GO; GO:0004534; F:5'-3' exoribonuclease activity; IEA:EnsemblFungi.
DR GO; GO:0019843; F:rRNA binding; IEA:EnsemblFungi.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1901408; P:negative regulation of phosphorylation of RNA polymerase II C-terminal domain; IEA:EnsemblFungi.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IEA:EnsemblFungi.
DR GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IEA:EnsemblFungi.
DR GO; GO:0034428; P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 5'-3'; IEA:EnsemblFungi.
DR GO; GO:0051984; P:positive regulation of chromosome segregation; IEA:EnsemblFungi.
DR GO; GO:1904595; P:positive regulation of termination of RNA polymerase II transcription; IEA:EnsemblFungi.
DR GO; GO:0043144; P:sno(s)RNA processing; IEA:EnsemblFungi.
DR GO; GO:0030847; P:termination of RNA polymerase II transcription, exosome-dependent; IEA:EnsemblFungi.
DR GO; GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IEA:EnsemblFungi.
DR GO; GO:0106354; P:tRNA surveillance; IEA:EnsemblFungi.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR004859; Put_53exo.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR017151; Xrn2/3/4.
DR PANTHER; PTHR12341; PTHR12341; 1.
DR Pfam; PF17846; XRN_M; 2.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF037239; Exonuclease_Xrn2; 1.
PE 3: Inferred from homology;
KW Exonuclease; Hydrolase; mRNA processing; Nuclease; Nucleus;
KW Reference proteome; rRNA processing; Transcription;
KW Transcription regulation; Transcription termination.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..992
FT /note="5'-3' exoribonuclease 2"
FT /id="PRO_0000249927"
FT REGION 528..568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..992
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..545
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 546..563
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 916..980
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 992 AA; 114291 MW; CAB1090F1953ED9B CRC64;
MGVPSFFRWL SRKYPKIISP VLEEYPVIED GVQLPLDYSS ANPNGELDNL YLDMNGIVHP
CSHPENKPPP ETEDEMLLAV FEYTNRVLNM ARPRKVLMIA VDGVAPRAKM NQQRARRFRS
ARDAKLQNEA REQVLRERED YGETIDENVK SKKTWDSNAI TPGTPFMDKL ATALRYWTSF
KLATDPGWKN LQIIISDATV PGEGEHKIMN FIRSQRADTQ YNPNTTHCIY GLDADLIFLG
LATHEPHFKI LREDVFANNN YKKPKPQDMI NLSEEEKQQL IQQDSEKPFL WLHISVLREY
LSAELAIPHL SFQFDFERAI DDWVFMCFFC GNDFLPHLPC LDVRENSIDI LVDIWKTVLP
KTKTYLTCDG TLNLEPVEAL LEQLGDRETD LFKKKYIQEV RKQEAHDRRK KLKSNPNVSQ
GKVDRNFMIP LENMPVYDVD GNAAEGSLNL SNKDFANMRK EINLANEGDG EAAKALKLKS
EKNSGVVEFS SEELKNSVQL SKTANYDAAT SLQEKLIAKK MAMRDEENAE ELSLKRKSED
VDSAEKETSN EPEDDEADYD EDEGSTVPPA VIHTGIVKSG FIDTDESVRL YEPGYHDRYY
QHKFHVPAKD IPALQKDVIR CYVEGISWVL LYYYQGCASW TWYYPYHYAP FAQDFKNIKN
LDIHFDLGEP FLPYEQLMSV LPAASGHALP EIFRPLMSDP NSEIIDFYPE EFPVDMNGKK
MAWQGIALLP FIDETRLLKT VREQYSKLSD SEKARNVRKK DALLISNKNV NYDLFMKNLY
GENPVNVIEF RHFKSGLSGF VTQAEEGFEL NSKLICPING GGLPDLSTNL FLKLSYTQPV
VAGRCKSLVL NGYIPPQPML TPQDRDCIIY KYSNRWNPSM MKYNIVPVGP SGITQYQPRV
GGYRSFFFHK EQTAYAQQPQ QNREYMHSQQ RQPQGSRYQQ SRYNNGNYNN SNNGYSDNNN
GNTGKYNRHY NNSRGNSNRY SNSNDRRREF RR
