ID   XRN2_NEUCR              Reviewed;        1072 AA.
AC   Q8WZX5;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=5'-3' exoribonuclease 2;
DE            EC=3.1.13.-;
DE   AltName: Full=Exoribonuclease 1;
GN   Name=exr-1; Synonyms=rat1; ORFNames=B7K22.110, NCU01643;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. Required for the
CC       processing of nuclear mRNA and rRNA precursors. May promote termination
CC       of transcription by RNA polymerase II (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AL670543; CAD21516.1; -; Genomic_DNA.
DR   EMBL; CM002237; EAA27029.1; -; Genomic_DNA.
DR   RefSeq; XP_956265.1; XM_951172.2.
DR   AlphaFoldDB; Q8WZX5; -.
DR   SMR; Q8WZX5; -.
DR   STRING; 5141.EFNCRP00000001742; -.
DR   EnsemblFungi; EAA27029; EAA27029; NCU01643.
DR   GeneID; 3872420; -.
DR   KEGG; ncr:NCU01643; -.
DR   VEuPathDB; FungiDB:NCU01643; -.
DR   HOGENOM; CLU_006038_1_1_1; -.
DR   InParanoid; Q8WZX5; -.
DR   Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004534; F:5'-3' exoribonuclease activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IBA:GO_Central.
DR   GO; GO:0034428; P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 5'-3'; IEA:EnsemblFungi.
DR   GO; GO:0051984; P:positive regulation of chromosome segregation; IEA:EnsemblFungi.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0106354; P:tRNA surveillance; IEA:EnsemblFungi.
DR   InterPro; IPR027073; 5_3_exoribonuclease.
DR   InterPro; IPR004859; Put_53exo.
DR   InterPro; IPR041412; Xrn1_helical.
DR   InterPro; IPR017151; Xrn2/3/4.
DR   PANTHER; PTHR12341; PTHR12341; 1.
DR   Pfam; PF17846; XRN_M; 1.
DR   Pfam; PF03159; XRN_N; 1.
DR   PIRSF; PIRSF037239; Exonuclease_Xrn2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Exonuclease; Hydrolase; Metal-binding; mRNA processing;
KW   Nuclease; Nucleus; Reference proteome; rRNA processing; Transcription;
KW   Transcription regulation; Transcription termination; Zinc; Zinc-finger.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..1072
FT                   /note="5'-3' exoribonuclease 2"
FT                   /id="PRO_0000249928"
FT   ZN_FING         269..286
FT                   /note="CCHC-type"
FT   REGION          414..459
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          509..577
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          865..911
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          943..1072
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          118..144
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        414..439
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        509..523
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        872..886
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        955..984
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1019..1042
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1072 AA;  118310 MW;  76C6549CCB87CFCD CRC64;
     MGIPAAFRWL STKYPKIISP VIEDQPITMP DGTVIPVDAT KPNPNGEEFD NLYLDMNGIV
     HPCSHPEDRP APKDEEEMMV EVFKYTDRVV NMVRPRKLLM IAVDGVAPRA KMNQQRSRRF
     RAAREAMEKE EDKQKFVELL KKQNGKPQEE EPVEVVVKKA FDSNSITPGT PFMDILAASL
     RYWCSYKLNT DPAWANIKVI ISDATVPGEG EHKIMEFVRS QRGSPNHDPN TRHVIYGLDA
     DLIMLGLATH EPHFRVLRED VFAQDSRPRL CKICGQKGHD AMNCKGEAKE KNGEFDEKDA
     VVPLKPFIWL HVSILREYLA IELDVPGLPF QFNLERAIDD WVFMCFFVGN DFLPHLPALE
     IRENGIDTLT AIWKDNLPVM GGYLTKDGHA DLERVQYIMA GLAKQEDAIF RRRKETEDRR
     EAGFKRRKLA DEARQRRAQG SRQIRQAAEN SGPPAGFSFH KINEFPGKAP QPSITHDMVV
     NRQAVDQANV ANKSAASVLK DQIKSMMAQG TSTPENGAES TETPAETAAA APATEEQAAP
     PAALGKRKAE LIEDAVAGPE ASSTEVTEIS ESDAEPTDSV RLWEEGYADR YYEQKFKVDR
     KDIDFRHKVG RAYAEGLAWV LEYYFQGCPS WEWFYPYHYA PFAADFVDLG KMEIKFEKGR
     ISRPFEQLMS VLPAASRHAI PEVFHSLMLE ETSEIRHFYP DDFEIDLNGK KMAWQGVALL
     PFIDMPRLLA AMETKSHLLS PEDHLRNAPG KDYLLLSDSH PTLYDHITSQ FYSKKQGTTK
     FELDPTKSAG LSGLVEKIDG YVPHGSLVYP LSRNSMPDVD YDRSLMVHYF FPDCSHTHKS
     MLLRGVQMPT PALTRSDIDI LKGKASRSGR SYGGVPLSNK NYSGRNEPIN YGPGGGQQGG
     RGRGGYQGGG GRGGGRGGYG GNGGGYQANG GGYYNGGNNG GGGGGYGGQP RDSYGPPPGW
     QPPPPPGLAG FGVGPPPPPP GAAYGGAPGG AGYGGYGSSR GGYQDNRYAL PPPPPQAYQA
     QGQQYGQGGS RGGYQGQGNQ YRGPPRQDRG YDNRGGYRGG GYRGGGDNRG YR