XRN2_YARLI
ID XRN2_YARLI Reviewed; 1010 AA.
AC Q6C961;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=5'-3' exoribonuclease 2;
DE EC=3.1.13.-;
GN Name=RAT1; OrderedLocusNames=YALI0D13750g;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIB 122 / E 150;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. Required for the
CC processing of nuclear mRNA and rRNA precursors. May promote termination
CC of transcription by RNA polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC subfamily. {ECO:0000305}.
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DR EMBL; CR382130; CAG80989.1; -; Genomic_DNA.
DR RefSeq; XP_502801.1; XM_502801.1.
DR AlphaFoldDB; Q6C961; -.
DR SMR; Q6C961; -.
DR STRING; 4952.CAG80989; -.
DR EnsemblFungi; CAG80989; CAG80989; YALI0_D13750g.
DR GeneID; 2910260; -.
DR KEGG; yli:YALI0D13750g; -.
DR VEuPathDB; FungiDB:YALI0_D13750g; -.
DR HOGENOM; CLU_006038_1_1_1; -.
DR InParanoid; Q6C961; -.
DR OMA; ETWEYIV; -.
DR Proteomes; UP000001300; Chromosome D.
DR GO; GO:0090730; C:Las1 complex; IEA:EnsemblFungi.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0110103; C:RNA polymerase II termination complex; IEA:EnsemblFungi.
DR GO; GO:0004534; F:5'-3' exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:EnsemblFungi.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1901408; P:negative regulation of phosphorylation of RNA polymerase II C-terminal domain; IEA:EnsemblFungi.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IEA:EnsemblFungi.
DR GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IEA:EnsemblFungi.
DR GO; GO:0000956; P:nuclear-transcribed mRNA catabolic process; IBA:GO_Central.
DR GO; GO:1904595; P:positive regulation of termination of RNA polymerase II transcription; IEA:EnsemblFungi.
DR GO; GO:0043144; P:sno(s)RNA processing; IEA:EnsemblFungi.
DR GO; GO:0030847; P:termination of RNA polymerase II transcription, exosome-dependent; IEA:EnsemblFungi.
DR GO; GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IEA:EnsemblFungi.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR004859; Put_53exo.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR017151; Xrn2/3/4.
DR PANTHER; PTHR12341; PTHR12341; 1.
DR Pfam; PF17846; XRN_M; 1.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF037239; Exonuclease_Xrn2; 1.
PE 3: Inferred from homology;
KW Exonuclease; Hydrolase; mRNA processing; Nuclease; Nucleus;
KW Reference proteome; rRNA processing; Transcription;
KW Transcription regulation; Transcription termination.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..1010
FT /note="5'-3' exoribonuclease 2"
FT /id="PRO_0000249930"
FT REGION 402..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 481..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 754..781
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 879..923
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 990..1010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 402..424
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..504
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..530
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1010 AA; 112939 MW; 749C8CCBC661C1DA CRC64;
MGVPGLYRHL SQKCPKIVSD VIEDETTIVD GEEIPGSDYS APNPNGELDN LYLDMNGIVH
PCTHPEGEEA PPSEDEMLLA VFKYTERVLN VCRPRKVLMM AIDGVAPRAK MNQQRARRFR
SAKDAAIEAE STLESELKAK TIELQSSGEK GKSLETILDE FKQGLVNKPG KWDSNAITPG
TPFMEKLAAA LRYWVGYKLN TEPGWRDLKV VISDATVPGE GEHKIMEFIR SQRGDPTYNA
NTSHCIYGLD ADLIFLGLAT HEPKFKLLRE DVFRQNKTAG HQKKPFIWLN IDILREYLRE
DLRFTHPQTK IPFDLERAID DWVFLCFFVG NDFLPHLPAC DVRDGSINVL VNQWKNGMTR
WKDYITCDGH VNLAQAQDLL KNFATQEQRI LVHQEQDRVE YEKSQANKAA KQQAHRDSKK
LRQQGEQAAL ESMPLYDTKG ESVGDVQLTN RELVGQRNDI TLANLANQDA ADKLRFMLKR
GSDTGAEEPA KRAKIEEEPK EAESNSSEAT PPTANPSIAN DADLSNPNVT VHETTGYADH
VQFHKSGYTE RYYMNKFHAK EDEVDNLRRQ VAEEYMKGVC WVLMYYYHGC QSWSWFYPYH
YAPLAGDFDQ ISNMKVEFGE SAPFRPYEQL MSVLPAASGH NLPAVFRPLM SEENSPIIDY
YPENFQLDLN GKKQEWKAVV LLPFINADHL LEHVQAKYTE LTPEEKDRNT LREEIVIFHR
LGKYAKTLEK RGLMEKGVIV SKKPLVLNSH ETSGLAGTVE RQNEADRTSS KPHLTFPLNV
STDQTMPSFE ADKNMAMTLE YKIPKLEYEN KSMLLQGYIP PKYTLSDEER EAIRNRERQR
RGGQQDSRLF SNTDHRQQQE LLMNGSYLAF KKLSLRDQPP PAKEDWMLND PKLAGDRGNG
GYNNRGGFNN HRGGRGGFNN RGRGGFNHGG GGFNNSRGGY GGGGGGYGGG GGQGYGGGQG
YGGGQGYGGG QGYGGGGYGG GQGYGGGGYG GGYGGGHRGG HRGGRGGHRY
