CAP8_ARATH
ID CAP8_ARATH Reviewed; 571 AA.
AC Q8LBH2; Q8S8K2; Q9ZVE6;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Putative clathrin assembly protein At2g01600;
GN OrderedLocusNames=At2g01600; ORFNames=F2I9.22, T8O11.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 266-571.
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane, clathrin-coated pit {ECO:0000250}.
CC Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000250}. Note=Colocalized with clathrin in the Golgi
CC area. {ECO:0000250}.
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DR EMBL; AC005560; AAM15059.1; -; Genomic_DNA.
DR EMBL; AC006069; AAD12692.3; -; Genomic_DNA.
DR EMBL; CP002685; AEC05472.1; -; Genomic_DNA.
DR EMBL; AF424593; AAL11587.1; -; mRNA.
DR EMBL; AY096639; AAM20138.1; -; mRNA.
DR EMBL; AY133828; AAM91762.1; -; mRNA.
DR EMBL; AY087211; AAM67368.1; -; mRNA.
DR PIR; G84426; G84426.
DR RefSeq; NP_565267.1; NM_126221.4.
DR AlphaFoldDB; Q8LBH2; -.
DR SMR; Q8LBH2; -.
DR STRING; 3702.AT2G01600.1; -.
DR iPTMnet; Q8LBH2; -.
DR PaxDb; Q8LBH2; -.
DR PRIDE; Q8LBH2; -.
DR ProteomicsDB; 240278; -.
DR EnsemblPlants; AT2G01600.1; AT2G01600.1; AT2G01600.
DR GeneID; 814689; -.
DR Gramene; AT2G01600.1; AT2G01600.1; AT2G01600.
DR KEGG; ath:AT2G01600; -.
DR Araport; AT2G01600; -.
DR TAIR; locus:2049587; AT2G01600.
DR eggNOG; KOG0251; Eukaryota.
DR HOGENOM; CLU_014098_2_0_1; -.
DR InParanoid; Q8LBH2; -.
DR OMA; VAWDYSS; -.
DR OrthoDB; 553740at2759; -.
DR PhylomeDB; Q8LBH2; -.
DR PRO; PR:Q8LBH2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LBH2; baseline and differential.
DR Genevisible; Q8LBH2; AT.
DR GO; GO:0005905; C:clathrin-coated pit; IBA:GO_Central.
DR GO; GO:0030136; C:clathrin-coated vesicle; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005545; F:1-phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0030276; F:clathrin binding; IDA:TAIR.
DR GO; GO:0032050; F:clathrin heavy chain binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IPI:TAIR.
DR GO; GO:0048268; P:clathrin coat assembly; IEA:InterPro.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IDA:TAIR.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR Gene3D; 1.20.58.150; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR011417; ANTH_dom.
DR InterPro; IPR014712; ANTH_dom_sf.
DR InterPro; IPR045192; AP180-like.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR PANTHER; PTHR22951; PTHR22951; 1.
DR Pfam; PF07651; ANTH; 1.
DR SMART; SM00273; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
PE 2: Evidence at transcript level;
KW Coated pit; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane;
KW Reference proteome.
FT CHAIN 1..571
FT /note="Putative clathrin assembly protein At2g01600"
FT /id="PRO_0000187074"
FT DOMAIN 24..161
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT REGION 325..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..571
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..346
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..535
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 542..571
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 380
FT /note="W -> L (in Ref. 4; AAM67368)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="A -> T (in Ref. 4; AAM67368)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 571 AA; 63629 MW; AA1FCCC1C4A7F048 CRC64;
MGTLQSWRKA YGALKDSTKV GLVRVNSEYA DLDVAIVKAT NHVECPPKDR HLRKIFAATS
VTRARADVAY CIHALSRRLH KTRNWTVALK TLIVIHRLLR EGDPTFREEL LNFSQRGRIL
QLSNFKDDSS PIAWDCSAWV RTYALFLEER LECFRVLKYD TEAERLPKSN PGQDKGYSRT
RDLDGEELLE QLPALQQLLY RLIGCRPEGA ANHNHVIQYA LALVLKESFK VYCAINDGII
NLIDKFFEMA KHEAITSLEI YKRAGQQARS LSDFYEACKG LELARNFQFP VLREPPQSFL
TTMEEYIKEA PRVVDVPAEP LLLTYRPDDG LTTEDTEPSH EEREMLPSDD VVVVSEETEP
SPPPPPSANA QNFIDTDDLW GLNTGAPDTS VIEDQNALAL AIVSTDADPP TPHFGQPNNY
DPTGWELALV TAPSSDISAS TERKLAGGLD TLTLSSLYDD GAYIASQRPV YGAPAPNPFA
SHDPFASSNG TAPPPQQQAV NNPFGAYQQT YQHQPQPTYQ HQSNPPTNNS NPFGDFGEFP
VNPVSQQPNT SGYGDFSVNQ HNNPFRSTGL I
