XTH1_SOLLC
ID XTH1_SOLLC Reviewed; 296 AA.
AC Q40144;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable xyloglucan endotransglucosylase/hydrolase 1;
DE Short=LeXTH1;
DE EC=2.4.1.207;
DE Flags: Precursor;
GN Name=XTH1; Synonyms=EXT;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8244968; DOI=10.1016/s0021-9258(19)74400-7;
RA Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E.,
RA Nishitani K.;
RT "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a
RT novel class of glycosyltransferase that mediates molecular grafting between
RT matrix polysaccharides in plant cell walls.";
RL J. Biol. Chem. 268:25364-25368(1993).
CC -!- FUNCTION: Catalyzes xyloglucan endohydrolysis (XEH) and/or
CC endotransglycosylation (XET). Cleaves and religates xyloglucan
CC polymers, an essential constituent of the primary cell wall, and
CC thereby participates in cell wall construction of growing tissues (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=breaks a beta-(1->4) bond in the backbone of a xyloglucan and
CC transfers the xyloglucanyl segment on to O-4 of the non-reducing
CC terminal glucose residue of an acceptor, which can be a xyloglucan or
CC an oligosaccharide of xyloglucan.; EC=2.4.1.207;
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Secreted,
CC extracellular space, apoplast {ECO:0000305}.
CC -!- PTM: Contains at least one intrachain disulfide bond essential for its
CC enzymatic activity. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. XTH group 1
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D16456; BAA03923.1; -; mRNA.
DR PIR; D49539; D49539.
DR RefSeq; NP_001233858.1; NM_001246929.2.
DR AlphaFoldDB; Q40144; -.
DR SMR; Q40144; -.
DR STRING; 4081.Solyc01g099630.2.1; -.
DR CAZy; GH16; Glycoside Hydrolase Family 16.
DR PaxDb; Q40144; -.
DR PRIDE; Q40144; -.
DR EnsemblPlants; Solyc01g099630.3.1; Solyc01g099630.3.1; Solyc01g099630.3.
DR GeneID; 544272; -.
DR Gramene; Solyc01g099630.3.1; Solyc01g099630.3.1; Solyc01g099630.3.
DR KEGG; sly:544272; -.
DR eggNOG; ENOG502QQ71; Eukaryota.
DR HOGENOM; CLU_048041_2_1_1; -.
DR InParanoid; Q40144; -.
DR OMA; HHIKFLN; -.
DR OrthoDB; 1209387at2759; -.
DR PhylomeDB; Q40144; -.
DR BRENDA; 2.4.1.207; 3101.
DR BRENDA; 3.2.1.151; 3101.
DR Proteomes; UP000004994; Chromosome 1.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0030247; F:polysaccharide binding; ISS:UniProtKB.
DR GO; GO:0016762; F:xyloglucan:xyloglucosyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0042546; P:cell wall biogenesis; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010411; P:xyloglucan metabolic process; IEA:InterPro.
DR CDD; cd02176; GH16_XET; 1.
DR InterPro; IPR044791; Beta-glucanase/XTH.
DR InterPro; IPR008264; Beta_glucanase.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR000757; GH16.
DR InterPro; IPR008263; GH16_AS.
DR InterPro; IPR010713; XET_C.
DR InterPro; IPR016455; XTH.
DR PANTHER; PTHR31062; PTHR31062; 1.
DR Pfam; PF00722; Glyco_hydro_16; 1.
DR Pfam; PF06955; XET_C; 1.
DR PIRSF; PIRSF005604; XET; 1.
DR PRINTS; PR00737; GLHYDRLASE16.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS01034; GH16_1; 1.
DR PROSITE; PS51762; GH16_2; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Cell wall; Cell wall biogenesis/degradation; Disulfide bond;
KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal;
KW Transferase.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..296
FT /note="Probable xyloglucan endotransglucosylase/hydrolase
FT 1"
FT /id="PRO_0000011835"
FT DOMAIN 23..221
FT /note="GH16"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01098"
FT ACT_SITE 107
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064"
FT ACT_SITE 111
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064"
FT BINDING 111
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT BINDING 124..126
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT BINDING 134..136
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT BINDING 200..201
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT BINDING 205
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT BINDING 282
FT /ligand="xyloglucan"
FT /ligand_id="ChEBI:CHEBI:18233"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT SITE 109
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 229..240
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
FT DISULFID 277..290
FT /evidence="ECO:0000250|UniProtKB:Q8GZD5"
SQ SEQUENCE 296 AA; 34011 MW; B4BD0A255406F444 CRC64;
MGIIKGVLFS IVLINLSLVV FCGYPRRPVD VPFWKNYEPS WASHHIKFLN GGTTTDLILD
RSSGAGFQSK KSYLFGHFSM KMRLVGGDSA GVVTAFYLSS NNAEHDEIDF EFLGNRTGQP
YILQTNVFTG GKGNREQRIY LWFDPTKGYH SYSVLWNTYL IVIFVDDVPI RAFKNSKDLG
VKFPFNQPMK IYSSLWDADD WATRGGLEKT NWANAPFTAS YTSFHVDGCE AATPQEVQVC
NTKGMKWWDQ KAFQDLDALQ YRRLRWVRQK YTVYNYCTDK ARYPVPPPEC TKDRDI
