XXT1_ORYSJ
ID XXT1_ORYSJ Reviewed; 448 AA.
AC Q10MQ0; Q0DSM1;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Probable xyloglucan 6-xylosyltransferase 1 {ECO:0000305};
DE Short=OsXXT1 {ECO:0000303|PubMed:24834920};
DE EC=2.4.2.39 {ECO:0000305};
DE AltName: Full=Proteinn SHORT ROOT HAIR 2 {ECO:0000303|PubMed:24834920};
GN Name=XXT1; Synonyms=SRH2 {ECO:0000303|PubMed:24834920};
GN OrderedLocusNames=Os03g0300000 {ECO:0000312|EMBL:BAF11767.1},
GN LOC_Os03g18820 {ECO:0000312|EMBL:ABF95475.1};
GN ORFNames=OsJ_27597 {ECO:0000312|EMBL:EAZ43011.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-448.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP INDUCTION.
RX PubMed=24042130; DOI=10.1016/j.gene.2013.09.004;
RA Lu Y.C., Yang S.N., Zhang J.J., Zhang J.J., Tan L.R., Yang H.;
RT "A collection of glycosyltransferases from rice (Oryza sativa) exposed to
RT atrazine.";
RL Gene 531:243-252(2013).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=24834920; DOI=10.1093/jxb/eru189;
RA Wang C., Li S., Ng S., Zhang B., Zhou Y., Whelan J., Wu P., Shou H.;
RT "Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair
RT development in Oryza sativa.";
RL J. Exp. Bot. 65:4149-4157(2014).
CC -!- FUNCTION: Probable xyloglucan xylosyltransferase involved in the
CC biosynthesis of xyloglucan in roots. {ECO:0000269|PubMed:24834920}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a
CC glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-
CC glucose linkage.; EC=2.4.2.39; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips, leaves, inflorescences and
CC panicles. {ECO:0000269|PubMed:24834920}.
CC -!- INDUCTION: Down-regulated by treatment with atrazine.
CC {ECO:0000269|PubMed:24042130}.
CC -!- DISRUPTION PHENOTYPE: Root hair phenotype, characterized by short root
CC hairs with bubble-like extrusions at the tip.
CC {ECO:0000269|PubMed:24834920}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF11767.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DP000009; ABF95475.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11767.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000145; EAZ43011.1; -; Genomic_DNA.
DR EMBL; AK060863; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015631442.1; XM_015775956.1.
DR AlphaFoldDB; Q10MQ0; -.
DR SMR; Q10MQ0; -.
DR STRING; 4530.OS03T0300000-01; -.
DR CAZy; GT34; Glycosyltransferase Family 34.
DR PaxDb; Q10MQ0; -.
DR PRIDE; Q10MQ0; -.
DR GeneID; 4332564; -.
DR KEGG; osa:4332564; -.
DR eggNOG; KOG4748; Eukaryota.
DR OrthoDB; 1223199at2759; -.
DR PlantReactome; R-OSA-5655101; Xyloglucan biosynthesis.
DR PlantReactome; R-OSA-9639861; Development of root hair.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10MQ0; OS.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IBA:GO_Central.
DR GO; GO:0033843; F:xyloglucan 6-xylosyltransferase activity; IBA:GO_Central.
DR GO; GO:0048767; P:root hair elongation; IMP:UniProtKB.
DR GO; GO:0009969; P:xyloglucan biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR008630; Glyco_trans_34.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31311; PTHR31311; 1.
DR Pfam; PF05637; Glyco_transf_34; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..448
FT /note="Probable xyloglucan 6-xylosyltransferase 1"
FT /id="PRO_0000434323"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 20..42
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..448
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT REGION 71..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 421
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 448 AA; 51646 MW; DA26D68569A36EB6 CRC64;
MWVAERVVGE RRMREIQRFA RNAKLTVVCL LLTVVVLRGT VGAGKFGTPQ QDLIELRHRF
ISHPHRALAE HHDALSRGGG SSSSSGRAAQ RDDEPDPPPR TLRDPPYTLG PKISDWDEQR
AAWHRRHPET PPFVNDVKPR VLLVTGSSPK PCENPVGDHY LLKSIKNKMD YCRVHGLEIF
YNMALLDAEM AGFWAKLPLL RALLLAHPEI EFLWWMDSDA MFSDMAFELP WERYGPYNLI
MHGWDEMVYD DKNWIGLNTG SFLLRNCQWS LDFLDTWAPM GPKGPVRIEA GKVLTKYLKD
RPVFEADDQS AMVYILATER EKWGDKVYLE NGYYLHGYWG ILVDRYEEML ENYHPGLGDH
RWPLVTHFVG CKPCGKFGDY PVERCLKQME RAFNFGDNQI LQMYGFTHKS LGSRKVKRIR
NETSNPLDVK DELGLLHPAF KAMKTTST
