XXT3_ARATH
ID XXT3_ARATH Reviewed; 457 AA.
AC Q9LF80; Q0WT14;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Probable xyloglucan 6-xylosyltransferase 3 {ECO:0000305};
DE EC=2.4.2.39 {ECO:0000305};
DE AltName: Full=Putative glycosyltransferase 3;
DE Short=AtGT3;
DE EC=2.4.-.-;
GN Name=XXT3 {ECO:0000305}; Synonyms=GT3, GTL7; OrderedLocusNames=At5g07720;
GN ORFNames=MBK20.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Mogelsvang S., Dupree P.;
RT "A novel Arabidopsis Golgi glycosyltransferase.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12032363; DOI=10.1073/pnas.102644799;
RA Faik A., Price N.J., Raikhel N.V., Keegstra K.;
RT "An Arabidopsis gene encoding an alpha-xylosyltransferase involved in
RT xyloglucan biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:7797-7802(2002).
CC -!- FUNCTION: Probable xyloglucan xylosyltransferase involved in the
CC biosynthesis of xyloglucan. {ECO:0000250|UniProtKB:Q9CA75}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a
CC glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-
CC glucose linkage.; EC=2.4.2.39; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KJ138699; AHL38639.1; -; mRNA.
DR EMBL; AJ245573; CAC01676.1; -; mRNA.
DR EMBL; AB010070; BAB11451.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91197.1; -; Genomic_DNA.
DR EMBL; BT010365; AAQ56808.1; -; mRNA.
DR EMBL; AK227750; BAE99734.1; -; mRNA.
DR RefSeq; NP_196389.1; NM_120854.3.
DR AlphaFoldDB; Q9LF80; -.
DR SMR; Q9LF80; -.
DR STRING; 3702.AT5G07720.1; -.
DR CAZy; GT34; Glycosyltransferase Family 34.
DR PaxDb; Q9LF80; -.
DR PRIDE; Q9LF80; -.
DR ProteomicsDB; 242380; -.
DR EnsemblPlants; AT5G07720.1; AT5G07720.1; AT5G07720.
DR GeneID; 830665; -.
DR Gramene; AT5G07720.1; AT5G07720.1; AT5G07720.
DR KEGG; ath:AT5G07720; -.
DR Araport; AT5G07720; -.
DR TAIR; locus:2160369; AT5G07720.
DR eggNOG; KOG4748; Eukaryota.
DR HOGENOM; CLU_034328_1_0_1; -.
DR InParanoid; Q9LF80; -.
DR OMA; RNETTFP; -.
DR OrthoDB; 629667at2759; -.
DR PhylomeDB; Q9LF80; -.
DR BioCyc; ARA:AT5G07720-MON; -.
DR PRO; PR:Q9LF80; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LF80; baseline and differential.
DR Genevisible; Q9LF80; AT.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR GO; GO:0033843; F:xyloglucan 6-xylosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009969; P:xyloglucan biosynthetic process; IBA:GO_Central.
DR GO; GO:0010411; P:xyloglucan metabolic process; IMP:TAIR.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR008630; Glyco_trans_34.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31311; PTHR31311; 1.
DR Pfam; PF05637; Glyco_transf_34; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..457
FT /note="Probable xyloglucan 6-xylosyltransferase 3"
FT /id="PRO_0000215171"
FT TOPO_DOM 1..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..71
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..457
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 431
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 457 AA; 52367 MW; 20C09D09C77B0132 CRC64;
MGKEDGFRTQ KRVSTASSAA AGVLPTTMAS GGVRRPPPRG RQIQKTFNNV KMTILCGFVT
ILVLRGTIGI NFGTSDADVV NQNIIEETNR LLAEIRSDSD PTDSNEPPDS DLDLNMTYTL
GPKITNWDQK RKLWLTQNPD FPSFINGKAK VLLLTGSPPK PCDNPIGDHY LLKSVKNKID
YCRIHGIEIV YNMAHLDKEL AGYWAKLPMI RRLMLSHPEI EWIWWMDSDA LFTDMVFEIP
LSRYENHNLV IHGYPDLLFD QKSWIALNTG SFLFRNCQWS LDLLDAWAPM GPKGPIREEA
GKILTANLKG RPAFEADDQS ALIYLLLSQK ETWMEKVFVE NQYYLHGFWE GLVDKYEEMM
EKYHPGLGDE RWPFITHFVG CKPCGSYADY AVERCLKSME RAFNFADNQV LKLYGFGHRG
LLSPKIKRIR NETTFPLKFV DRFDIRRTTP LKIEARS
