XXT4_ARATH
ID XXT4_ARATH Reviewed; 513 AA.
AC Q9M9U0; Q682Q1; W8PW22;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Xyloglucan 6-xylosyltransferase 4 {ECO:0000305};
DE EC=2.4.2.39 {ECO:0000269|PubMed:22670626};
DE AltName: Full=Putative glycosyltransferase 4;
DE Short=AtGT4;
DE EC=2.4.-.-;
GN Name=XXT4 {ECO:0000303|PubMed:22670626}; Synonyms=GT4;
GN OrderedLocusNames=At1g18690; ORFNames=F6A14.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12032363; DOI=10.1073/pnas.102644799;
RA Faik A., Price N.J., Raikhel N.V., Keegstra K.;
RT "An Arabidopsis gene encoding an alpha-xylosyltransferase involved in
RT xyloglucan biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:7797-7802(2002).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=22670626; DOI=10.1111/j.1469-8137.2012.04196.x;
RA Vuttipongchaikij S., Brocklehurst D., Steele-King C., Ashford D.A.,
RA Gomez L.D., McQueen-Mason S.J.;
RT "Arabidopsis GT34 family contains five xyloglucan alpha-1,6-
RT xylosyltransferases.";
RL New Phytol. 195:585-595(2012).
CC -!- FUNCTION: Xylosyltransferase specific to UDP-D-xylose that accepts
CC cellohexaose as substrate to produce xyloglucan.
CC {ECO:0000269|PubMed:22670626}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a
CC glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-
CC glucose linkage.; EC=2.4.2.39;
CC Evidence={ECO:0000269|PubMed:22670626};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC {ECO:0000305}.
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DR EMBL; KJ139001; AHL38941.1; -; mRNA.
DR EMBL; AC011809; AAF27110.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29746.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58179.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58180.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58181.1; -; Genomic_DNA.
DR EMBL; AK175316; BAD43079.1; -; mRNA.
DR EMBL; AK229765; BAF01601.1; -; mRNA.
DR PIR; F86320; F86320.
DR RefSeq; NP_001319036.1; NM_001332359.1.
DR RefSeq; NP_001320634.1; NM_001332361.1.
DR RefSeq; NP_001320635.1; NM_001332360.1.
DR RefSeq; NP_173304.2; NM_101727.3.
DR AlphaFoldDB; Q9M9U0; -.
DR SMR; Q9M9U0; -.
DR BioGRID; 23689; 1.
DR STRING; 3702.AT1G18690.1; -.
DR CAZy; GT34; Glycosyltransferase Family 34.
DR PaxDb; Q9M9U0; -.
DR PRIDE; Q9M9U0; -.
DR ProteomicsDB; 242409; -.
DR EnsemblPlants; AT1G18690.1; AT1G18690.1; AT1G18690.
DR EnsemblPlants; AT1G18690.2; AT1G18690.2; AT1G18690.
DR EnsemblPlants; AT1G18690.3; AT1G18690.3; AT1G18690.
DR EnsemblPlants; AT1G18690.4; AT1G18690.4; AT1G18690.
DR GeneID; 838450; -.
DR Gramene; AT1G18690.1; AT1G18690.1; AT1G18690.
DR Gramene; AT1G18690.2; AT1G18690.2; AT1G18690.
DR Gramene; AT1G18690.3; AT1G18690.3; AT1G18690.
DR Gramene; AT1G18690.4; AT1G18690.4; AT1G18690.
DR KEGG; ath:AT1G18690; -.
DR Araport; AT1G18690; -.
DR TAIR; locus:2035030; AT1G18690.
DR eggNOG; KOG4748; Eukaryota.
DR HOGENOM; CLU_034328_1_0_1; -.
DR InParanoid; Q9M9U0; -.
DR OMA; KVTRWNA; -.
DR OrthoDB; 629667at2759; -.
DR PhylomeDB; Q9M9U0; -.
DR BioCyc; ARA:AT1G18690-MON; -.
DR PRO; PR:Q9M9U0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9U0; baseline and differential.
DR Genevisible; Q9M9U0; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IDA:TAIR.
DR GO; GO:0033843; F:xyloglucan 6-xylosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009969; P:xyloglucan biosynthetic process; IBA:GO_Central.
DR GO; GO:0010411; P:xyloglucan metabolic process; IDA:TAIR.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR008630; Glyco_trans_34.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31311; PTHR31311; 4.
DR Pfam; PF05637; Glyco_transf_34; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..513
FT /note="Xyloglucan 6-xylosyltransferase 4"
FT /id="PRO_0000215172"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..513
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 490
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 95
FT /note="H -> Y (in Ref. 4; BAF01601/BAD43079)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 513 AA; 58314 MW; ABA6ECC18912B275 CRC64;
MFQDGSRSSG SGRGLSTTAV SNGGWRTRGF LRGWQIQNTL FNNIKFMILC CFVTILILLG
TIRVGNLGSS NADSVNQSFI KETIPILAEI PSDSHSTDLA EPPKADVSPN ATYTLEPRIA
EIPSDVHSTD LVELPKADIS PNATYTLGPR IAEIPSDSHL TDLLEPPKAD ISPNATYTLG
PKITNWDSQR KVWLNQNPEF PNIVNGKARI LLLTGSSPGP CDKPIGNYYL LKAVKNKIDY
CRLHGIEIVY NMANLDEELS GYWTKLPMIR TLMLSHPEVE WIWWMDSDAL FTDILFEIPL
PRYENHNLVI HGYPDLLFNQ KSWVALNTGI FLLRNCQWSL DLLDAWAPMG PKGKIRDETG
KILTAYLKGR PAFEADDQSA LIYLLLSQKE KWIEKVYVEN QYYLHGFWEG LVDRYEEMIE
KYHPGLGDER WPFVTHFVGC KPCGSYADYA VDRCFKSMER AFNFADNQVL KLYGFSHRGL
LSPKIKRIRN ETVSPLESVD KFDIRRMHME TKP
