XYL1_CANTR
ID XYL1_CANTR Reviewed; 324 AA.
AC O13283;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=NAD(P)H-dependent D-xylose reductase I,II;
DE Short=XR;
DE EC=1.1.1.307;
GN Name=xyrA;
OS Candida tropicalis (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=5482;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96745 / CBS 4913 / NBRC 0618 / NRRL Y-5716;
RA Yokoyama S., Kinoshita Y., Suzuki T., Kawai K., Horitsu H., Takamizawa K.;
RT "Cloning and sequencing of two D-xylose reductase genes (xyrA and xyrB)
RT from Candida tropicalis.";
RL J. Ferment. Bioeng. 80:603-605(1995).
CC -!- FUNCTION: Reduces D-xylose into xylitol. Has a preference for NADPH,
CC but can also utilize NADH as cosubstrate (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + xylitol = D-xylose + H(+) + NADH;
CC Xref=Rhea:RHEA:27441, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC ChEBI:CHEBI:53455, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.307;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NADP(+) + xylitol = D-xylose + H(+) + NADPH;
CC Xref=Rhea:RHEA:27445, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC ChEBI:CHEBI:53455, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.307;
CC -!- PATHWAY: Carbohydrate metabolism; D-xylose degradation.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR EMBL; AB002105; BAA19476.1; -; Genomic_DNA.
DR AlphaFoldDB; O13283; -.
DR SMR; O13283; -.
DR PRIDE; O13283; -.
DR VEuPathDB; FungiDB:CTMYA2_054230; -.
DR VEuPathDB; FungiDB:CTRG_05993; -.
DR UniPathway; UPA00810; -.
DR GO; GO:0032866; F:D-xylose:NADP reductase activity; IEA:InterPro.
DR GO; GO:0042843; P:D-xylose catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd19113; AKR_AKR2B1-10; 1.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR044486; AKR2B1.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; SSF51430; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; NAD; NADP; Oxidoreductase; Xylose metabolism.
FT CHAIN 1..324
FT /note="NAD(P)H-dependent D-xylose reductase I,II"
FT /id="PRO_0000124662"
FT ACT_SITE 54
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 116
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 171..172
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 220..229
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 276..286
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT SITE 83
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 324 AA; 36575 MW; 3002FFBDA21FA3E0 CRC64;
MSTTPTIPTI KLNSGYEMPL VGFGCWKVTN ATAADQIYNA IKTGYRLFDG AEDYGNEKEV
GEGINRAIKE GLVKREELFI TSKLWNNFHD PKNVETALNK TLSDLNLDYV DLFLIHFPIA
FKFVPIEEKY PPGFYCGDGD NFHYEDVPLL DTWKALEKLV EAGKIKSIGI SNFTGALIYD
LIRGATIKPA VLQIEHHPYL QQPKLIEYVQ KAGIAITGYS SFGPQSFLEL ESKRALNTPT
LFEHETIKLI ADKHGKSPAQ VLLRWATQRN IAVIPKSNNP ERLAQNLSVV DFDLTKDDLD
NIAKLDIGLR FNDPWDWDNI PIFV
