XYL1_MAGO7
ID XYL1_MAGO7 Reviewed; 324 AA.
AC G4N708; Q3MSM7;
DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=NAD(P)H-dependent D-xylose reductase XYR1;
DE Short=XR;
DE EC=1.1.1.307;
GN Name=XYR1; ORFNames=MGG_03648;
OS Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS fungus) (Pyricularia oryzae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX NCBI_TaxID=242507;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC STRAIN=P1.2;
RX PubMed=23499935; DOI=10.1016/j.febslet.2013.03.003;
RA Klaubauf S., Ribot C., Melayah D., Lagorce A., Lebrun M.H., de Vries R.P.;
RT "The pentose catabolic pathway of the rice-blast fungus Magnaporthe oryzae
RT involves a novel pentose reductase restricted to few fungal species.";
RL FEBS Lett. 587:1346-1352(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX PubMed=15846337; DOI=10.1038/nature03449;
RA Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL Nature 434:980-986(2005).
CC -!- FUNCTION: Catalyzes the initial reaction in the xylose utilization
CC pathway by reducing D-xylose into xylitol. Xylose is a major component
CC of hemicelluloses such as xylan. Most fungi utilize D-xylose via three
CC enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase
CC (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters
CC pentose phosphate pathway (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + xylitol = D-xylose + H(+) + NADH;
CC Xref=Rhea:RHEA:27441, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC ChEBI:CHEBI:53455, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.1.1.307;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NADP(+) + xylitol = D-xylose + H(+) + NADPH;
CC Xref=Rhea:RHEA:27445, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC ChEBI:CHEBI:53455, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.307;
CC -!- PATHWAY: Carbohydrate metabolism; D-xylose degradation.
CC -!- INDUCTION: Overexpressed during growth on both D-xylose or L-arabinose
CC compared to D-glucose. Significantly expressed during late stages of
CC infection of barley leaves. {ECO:0000269|PubMed:23499935}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR EMBL; AJ890447; CAI67591.1; -; mRNA.
DR EMBL; CM001234; EHA49921.1; -; Genomic_DNA.
DR RefSeq; XP_003716240.1; XM_003716192.1.
DR AlphaFoldDB; G4N708; -.
DR SMR; G4N708; -.
DR STRING; 318829.MGG_03648T0; -.
DR EnsemblFungi; MGG_03648T0; MGG_03648T0; MGG_03648.
DR GeneID; 2676633; -.
DR KEGG; mgr:MGG_03648; -.
DR VEuPathDB; FungiDB:MGG_03648; -.
DR eggNOG; KOG1577; Eukaryota.
DR HOGENOM; CLU_023205_0_0_1; -.
DR InParanoid; G4N708; -.
DR OMA; ECGEGVA; -.
DR OrthoDB; 1016440at2759; -.
DR UniPathway; UPA00810; -.
DR Proteomes; UP000009058; Chromosome 4.
DR GO; GO:0032866; F:D-xylose:NADP reductase activity; IEA:InterPro.
DR GO; GO:0042843; P:D-xylose catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd19115; AKR_AKR2D1; 1.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR044487; AKR2D.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; SSF51430; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; NAD; NADP; Oxidoreductase; Reference proteome;
KW Xylose metabolism.
FT CHAIN 1..324
FT /note="NAD(P)H-dependent D-xylose reductase XYR1"
FT /id="PRO_0000425415"
FT ACT_SITE 50
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 168..169
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 217..226
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 273..283
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT SITE 79
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 324 AA; 36631 MW; EA7067EE14977862 CRC64;
MASPTMKLNN GLDMPQVGFG LWKVENSVCA DVVYNAIKAG YRLFDGACDY GNEVECGQGV
KRAIDEGLVK REELFIVSKL WNTFHDGERV EPIVKKQLAD WGIEYFDLYL IHFPVALEYV
DPSVRYPPGW HYDDAGTEIR PSKASIQETW TAMEKLVDAG LSKAIGVSNF QAQLLYDMLR
YARIRPATLQ IEHHPYLVQQ RLIEACKTEG IVVTAYSSFG PASFKEFNME HAEALTPLLE
EPTIVKLAEK YGKDPGQVLL RWATQRGLAV IPKSSREKTM KSNFEAVGWD MEDSDIKTIS
ALDKGIRFNQ PANYFSTDKL WIFG
