ID   XYL1_SCHSH              Reviewed;         323 AA.
AC   Q9P430;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=NAD(P)H-dependent D-xylose reductase;
DE            Short=XR;
DE            EC=1.1.1.307;
GN   Name=XYL1;
OS   Scheffersomyces shehatae (Yeast) (Candida shehatae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=45589;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CS051;
RA   Govinden R., Pillay B., Pillay D.;
RT   "Isolation and expression of the Candida shehatae xylose reductase gene
RT   (XYL1) in Saccharomyces cerevisiae.";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reduces D-xylose into xylitol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + xylitol = D-xylose + H(+) + NADH;
CC         Xref=Rhea:RHEA:27441, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC         ChEBI:CHEBI:53455, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.307;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + xylitol = D-xylose + H(+) + NADPH;
CC         Xref=Rhea:RHEA:27445, ChEBI:CHEBI:15378, ChEBI:CHEBI:17151,
CC         ChEBI:CHEBI:53455, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.307;
CC   -!- PATHWAY: Carbohydrate metabolism; D-xylose degradation.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; AF278715; AAF86345.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9P430; -.
DR   SMR; Q9P430; -.
DR   PRIDE; Q9P430; -.
DR   UniPathway; UPA00810; -.
DR   GO; GO:0032866; F:D-xylose:NADP reductase activity; IEA:InterPro.
DR   GO; GO:0042843; P:D-xylose catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd19113; AKR_AKR2B1-10; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044486; AKR2B1.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; NAD; Oxidoreductase; Xylose metabolism.
FT   CHAIN           1..323
FT                   /note="NAD(P)H-dependent D-xylose reductase"
FT                   /id="PRO_0000124660"
FT   ACT_SITE        53
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         219..285
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   SITE            82
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   323 AA;  36594 MW;  E5AB1A3141FF27E3 CRC64;
     MSPSPIPAFK LNNGLEMPSI GFGCWKLDKS TAADQVYNAI KAGYRLFDGA EDYGNEQEVG
     EGVKRAIDEG IVTREEIFLT SKLWNNYHDP KNVETALNKT LKDLKVDYVD LFLIHFPIAF
     KFVPIEEKYP PGFYCGDGDN FVYEDVPILE TWKALEKLVK AGKIRSIGVS NFPGALLLDL
     FRGATIKPAV LQVEHHPYLQ QPKLIEYAQK VGITVTAYSS FGPQSFVEMN QGRALNTPTL
     FEHDVIKAIA AKHNKVPAEV LLRWSAQRGI AVIPKSNLPE RLVQNRSFND FELTKEDFEE
     ISKLDINLRF NDPWDWDNIP IFV