XYLA_ARATH
ID XYLA_ARATH Reviewed; 477 AA.
AC Q9FKK7; Q8L759;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Xylose isomerase;
DE EC=5.3.1.5;
GN Name=XYLA; OrderedLocusNames=At5g57655; ORFNames=MUA2.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-xylose = alpha-D-xylulofuranose; Xref=Rhea:RHEA:22816,
CC ChEBI:CHEBI:28518, ChEBI:CHEBI:188998; EC=5.3.1.5;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FKK7-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the xylose isomerase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08802.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB011482; BAB08802.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96932.1; -; Genomic_DNA.
DR EMBL; AY136469; AAM97134.1; -; mRNA.
DR EMBL; BT006607; AAP31951.1; -; mRNA.
DR RefSeq; NP_568861.3; NM_125147.5. [Q9FKK7-1]
DR AlphaFoldDB; Q9FKK7; -.
DR SMR; Q9FKK7; -.
DR BioGRID; 21115; 5.
DR STRING; 3702.AT5G57655.2; -.
DR PaxDb; Q9FKK7; -.
DR PRIDE; Q9FKK7; -.
DR ProteomicsDB; 242513; -. [Q9FKK7-1]
DR EnsemblPlants; AT5G57655.2; AT5G57655.2; AT5G57655. [Q9FKK7-1]
DR GeneID; 835871; -.
DR Gramene; AT5G57655.2; AT5G57655.2; AT5G57655. [Q9FKK7-1]
DR KEGG; ath:AT5G57655; -.
DR Araport; AT5G57655; -.
DR TAIR; locus:505006696; AT5G57655.
DR eggNOG; ENOG502QRMS; Eukaryota.
DR HOGENOM; CLU_037261_1_0_1; -.
DR InParanoid; Q9FKK7; -.
DR OMA; TLAMYEI; -.
DR PhylomeDB; Q9FKK7; -.
DR BioCyc; ARA:AT5G57655-MON; -.
DR PRO; PR:Q9FKK7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKK7; baseline and differential.
DR Genevisible; Q9FKK7; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009045; F:xylose isomerase activity; IBA:GO_Central.
DR GO; GO:0042843; P:D-xylose catabolic process; IBA:GO_Central.
DR HAMAP; MF_00455; Xylose_isom_A; 1.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR InterPro; IPR013452; Xylose_isom_bac.
DR InterPro; IPR001998; Xylose_isomerase.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR PRINTS; PR00688; XYLOSISMRASE.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR02630; xylose_isom_A; 1.
DR PROSITE; PS51415; XYLOSE_ISOMERASE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carbohydrate metabolism; Isomerase; Magnesium;
KW Manganese; Metal-binding; Reference proteome; Xylose metabolism.
FT CHAIN 1..477
FT /note="Xylose isomerase"
FT /id="PRO_0000195824"
FT ACT_SITE 142
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 309
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 309
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 312
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 337
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 348
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 350
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 380
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
SQ SEQUENCE 477 AA; 53720 MW; 4C89078F3179AE0C CRC64;
MKKVEFFMLL LCFIAASSLV SADPPTCPAD LGGKCSDSDD WQGDFFPEIP KIKYEGPSSK
NPLAYRWYNA EEEILGKKMK DWFRFSVAFW HTFRGTGGDP FGAATKYWPW EDGTNSVSMA
KRRMRANFEF LKKLGVDWWC FHDRDIAPDG TTLEESNKNL DEVIELAKEL QKGSKIKPLW
GTAQLFLHPR YMHGGATSSE VGVYAYAAAQ VKKAMEVTHY LGGENYVFWG GREGYQTLLN
TDMGRELDHL ARFFEAAVAY KKKIGFKGTL LIEPKPQEPT KHQYDWDAAT AANFLRKYGL
IDEFKLNIEC NHATLSGHTC HHELETARIN GLLGNIDANT GDAQTGWDTD QFLTDVGEAT
MVMMSVIKNG GIAPGGFNFD AKLRRESTDV EDLFIAHISG MDTMARGLRN AVKILEEGSL
SELVRKRYAT WDSELGKQIE EGKADFEYLE KKAKEFGEPK VSSAKQELAE MIFQSAM
