XYLA_BACSU
ID XYLA_BACSU Reviewed; 445 AA.
AC P0CI80; P04788; P94491;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Xylose isomerase;
DE EC=5.3.1.5;
GN Name=xylA; OrderedLocusNames=BSU17600;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2994009; DOI=10.1093/nar/13.15.5717;
RA Wilhelm M., Hollenberg C.P.;
RT "Nucleotide sequence of the Bacillus subtilis xylose isomerase gene:
RT extensive homology between the Bacillus and Escherichia coli enzyme.";
RL Nucleic Acids Res. 13:5717-5722(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Borchert S., Klein C., Piksa B., Hammelmann M., Entian K.-D.;
RT "Sequencing of a 26 kb region of the Bacillus subtilis genome downstream of
RT spoVJ.";
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 214 AND 246.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-xylose = alpha-D-xylulofuranose; Xref=Rhea:RHEA:22816,
CC ChEBI:CHEBI:28518, ChEBI:CHEBI:188998; EC=5.3.1.5;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. {ECO:0000250};
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the xylose isomerase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26562.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X02795; CAA26562.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U66480; AAB41093.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13644.2; -; Genomic_DNA.
DR PIR; C69735; ISBSXS.
DR RefSeq; NP_389642.2; NC_000964.3.
DR RefSeq; WP_003245235.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P0CI80; -.
DR SMR; P0CI80; -.
DR STRING; 224308.BSU17600; -.
DR PaxDb; P0CI80; -.
DR PRIDE; P0CI80; -.
DR EnsemblBacteria; CAB13644; CAB13644; BSU_17600.
DR GeneID; 939558; -.
DR KEGG; bsu:BSU17600; -.
DR PATRIC; fig|224308.179.peg.1910; -.
DR eggNOG; COG2115; Bacteria.
DR InParanoid; P0CI80; -.
DR OMA; TLAMYEI; -.
DR PhylomeDB; P0CI80; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009045; F:xylose isomerase activity; IBA:GO_Central.
DR GO; GO:0042843; P:D-xylose catabolic process; IBA:GO_Central.
DR HAMAP; MF_00455; Xylose_isom_A; 1.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR InterPro; IPR013452; Xylose_isom_bac.
DR InterPro; IPR001998; Xylose_isomerase.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR PRINTS; PR00688; XYLOSISMRASE.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR02630; xylose_isom_A; 1.
DR PROSITE; PS51415; XYLOSE_ISOMERASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Cytoplasm; Isomerase; Magnesium; Metal-binding;
KW Reference proteome; Xylose metabolism.
FT CHAIN 1..445
FT /note="Xylose isomerase"
FT /id="PRO_0000195767"
FT ACT_SITE 107
FT /evidence="ECO:0000250"
FT ACT_SITE 110
FT /evidence="ECO:0000250"
FT BINDING 238
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 274
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 274
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 277
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 302
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 313
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 315
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 345
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT CONFLICT 9
FT /note="I -> V (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 15
FT /note="A -> V (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="Y -> F (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 24
FT /note="D -> A (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="L -> M (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 86..87
FT /note="KM -> RA (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 127..129
FT /note="MIM -> IIV (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 138..140
FT /note="NSG -> DSN (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 214
FT /note="N -> D (in Ref. 1; AAB41093)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="G -> E (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 246
FT /note="T -> A (in Ref. 1; AAB41093)"
FT /evidence="ECO:0000305"
FT CONFLICT 359
FT /note="I -> V (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 405
FT /note="I -> T (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 421..423
FT /note="HKS -> NKT (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="K -> R (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
FT CONFLICT 436
FT /note="A -> P (in Ref. 2; CAA26562)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 445 AA; 50282 MW; 6A7E37F25E135AFD CRC64;
MAQSHSSSIN YFGSANKVVY EGKDSTNPLA FKYYNPQEVI GGKTLKEHLR FSIAYWHTFT
ADGTDVFGAA TMQRPWDHYK GMDLAKMRVE AAFEMFEKLD APFFAFHDRD IAPEGSTLKE
TNQNLDMIMG MIKDYMRNSG VKLLWNTANM FTNPRFVHGA ATSCNADVFA YAAAQVKKGL
ETAKELGAEN YVFWGGREGY ETLLNTDLKF ELDNLARFMH MAVDYAKEIG YTGQFLIEPK
PKEPTTHQYD TDAATTIAFL KQYGLDNHFK LNLEANHATL AGHTFEHELR MARVHGLLGS
VDANQGHPLL GWDTDEFPTD LYSTTLAMYE ILQNGGLGSG GLNFDAKVRR SSFEPDDLIY
AHIAGMDAFA RGLKVAHKLI EDRVFEDVIQ HRYRSFTEGI GLEIIEGRAN FHTLEQYALN
HKSIKNESGR QEKLKAILNQ YILEV
