CAPA_BACAN
ID CAPA_BACAN Reviewed; 411 AA.
AC P19579; Q9RMX6;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 18-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Capsule biosynthesis protein CapA;
GN Name=capA; OrderedLocusNames=pXO2-56, BXB0064, GBAA_pXO2_0064;
OS Bacillus anthracis.
OG Plasmid pXO2, and Plasmid pTE702.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pTE702;
RX PubMed=2536679; DOI=10.1128/jb.171.2.722-730.1989;
RA Makino S., Uchida I., Terakado N., Sasakawa C., Yoshikawa M.;
RT "Molecular characterization and protein analysis of the cap region, which
RT is essential for encapsulation in Bacillus anthracis.";
RL J. Bacteriol. 171:722-730(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Pasteur; PLASMID=pXO2;
RX PubMed=10475962; DOI=10.1046/j.1365-2672.1999.00883.x;
RA Okinaka R.T., Cloud K., Hampton O., Hoffmaster A., Hill K.K., Keim P.,
RA Koehler T., Lamke G., Kumano S., Manter D., Martinez Y., Ricke D.,
RA Svensson R., Jackson P.J.;
RT "Sequence, assembly and analysis of pXO1 and pXO2.";
RL J. Appl. Microbiol. 87:261-262(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Ames / isolate Florida / A2012; PLASMID=pXO2;
RX PubMed=12004073; DOI=10.1126/science.1071837;
RA Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA Fraser C.M.;
RT "Comparative genome sequencing for discovery of novel polymorphisms in
RT Bacillus anthracis.";
RL Science 296:2028-2033(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor; PLASMID=pXO2;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-107.
RC STRAIN=PAI; PLASMID=pXO2;
RX PubMed=14985634;
RA Inoue S., Noguchi A., Tanabayashi K., Yamada A.;
RT "Preparation of a positive control DNA for molecular diagnosis of Bacillus
RT anthracis.";
RL Jpn. J. Infect. Dis. 57:29-32(2004).
RN [6]
RP INDUCTION.
RC PLASMID=pXO2;
RX PubMed=14702298; DOI=10.1128/jb.186.2.307-315.2004;
RA Drysdale M., Bourgogne A., Hilsenbeck S.G., Koehler T.M.;
RT "atxA controls Bacillus anthracis capsule synthesis via acpA and a newly
RT discovered regulator, acpB.";
RL J. Bacteriol. 186:307-315(2004).
CC -!- FUNCTION: Essential for the synthesis of the polyglutamate capsule of
CC B.anthracis which is one of the principal virulence factors during
CC anthrax infection. May form a polyglutamyl synthetase complex together
CC with proteins CapB and CapC.
CC -!- PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
CC -!- INDUCTION: Capsule synthesis is transcriptionally regulated by AtxA,
CC AcpA and AcpB. {ECO:0000269|PubMed:14702298}.
CC -!- SIMILARITY: Belongs to the CapA family. {ECO:0000305}.
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DR EMBL; M24150; AAA22288.1; -; Genomic_DNA.
DR EMBL; AF188935; AAF13661.1; -; Genomic_DNA.
DR EMBL; AE011191; AAM26220.1; -; Genomic_DNA.
DR EMBL; AE017335; AAT28994.2; -; Genomic_DNA.
DR EMBL; AB125962; BAD14938.1; -; Genomic_DNA.
DR PIR; C30091; C30091.
DR RefSeq; NP_053211.1; NC_002146.1.
DR RefSeq; WP_001253154.1; NZ_RXZJ01000072.1.
DR RefSeq; WP_001253155.1; NZ_VTZL01000009.1.
DR AlphaFoldDB; P19579; -.
DR EnsemblBacteria; AAT28994; AAT28994; GBAA_pXO2_0064.
DR GeneID; 45025365; -.
DR KEGG; bar:GBAA_pXO2_0064; -.
DR HOGENOM; CLU_038823_2_2_9; -.
DR OMA; HWGQEYD; -.
DR BioCyc; MetaCyc:MON-16979; -.
DR UniPathway; UPA00934; -.
DR Proteomes; UP000000594; Plasmid pXO2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07381; MPP_CapA; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR019079; Capsule_synth_CapA.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF09587; PGA_cap; 1.
DR SMART; SM00854; PGA_cap; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 2: Evidence at transcript level;
KW Capsule biogenesis/degradation; Cell membrane; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..411
FT /note="Capsule biosynthesis protein CapA"
FT /id="PRO_0000089307"
FT TRANSMEM 25..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 386..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..404
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 105
FT /note="E -> G (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="T -> A (in Ref. 1; AAA22288)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 411 AA; 46453 MW; C84572D0BB18DCC0 CRC64;
MRRKLTFQEK LLIFIKKTKK KNPRYVAIVL PLIAVILIAA TWVQRTEAVA PVKHRENEKL
TMTMVGDIMM GRHVKEIVNR YGTDYVFRHV SPYLKNSDYV SGNFEHPVLL EDKKNYQKAD
KNIHLSAKEE TVKAVKEAGF TVLNLANNHM TDYGAKGTKD TIKAFKEADL DYVGAGENFK
DVKNIVYQNV NGVRVATLGF TDAFVAGAIA TKEQPGSLSM NPDVLLKQIS KAKDPKKGNA
DLVVVNTHWG EEYDNKPSPR QEALAKAMVD AGADIIVGHH PHVLQSFDVY KQGIIFYSLG
NFVFDQGWTR TKDSALVQYH LRDNGTAILD VVPLNIQEGS PKPVTSALDK NRVYRQLTKD
TSKGALWSKK DDKLEIKLNH KHVIEKMKKR EKQEHQDKQE KENQVSVETT T
