CAPA_BACSU
ID CAPA_BACSU Reviewed; 380 AA.
AC P96738; Q795C8; Q9AJM1;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=PGA biosynthesis protein CapA;
GN Name=capA; Synonyms=pgsA, ywtB; OrderedLocusNames=BSU35880;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NBRC 3336;
RX PubMed=10486244; DOI=10.1006/bbrc.1999.1298;
RA Ashiuchi M., Soda K., Misono H.;
RT "A poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336:
RT gene cloning and biochemical analysis of poly-gammma-glutamate produced by
RT Escherichia coli clone cells.";
RL Biochem. Biophys. Res. Commun. 263:6-12(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Asahikawa;
RA Tran L.P., Itoh Y.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=NBRC 16449;
RX PubMed=11751809; DOI=10.1128/jb.184.2.337-343.2002;
RA Urushibata Y., Tokuyama S., Tahara Y.;
RT "Characterization of the Bacillus subtilis ywsC gene, involved in gamma-
RT polyglutamic acid production.";
RL J. Bacteriol. 184:337-343(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Seems to be required for maximum PGA (gamma-polyglutamic
CC acid) production.
CC -!- DISRUPTION PHENOTYPE: Cells produce PGA but at a greatly reduced level.
CC {ECO:0000269|PubMed:11751809}.
CC -!- SIMILARITY: Belongs to the CapA family. {ECO:0000305}.
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DR EMBL; Z92954; CAB07469.1; -; Genomic_DNA.
DR EMBL; AB016245; BAA85265.1; -; Genomic_DNA.
DR EMBL; AB039950; BAB13486.1; -; Genomic_DNA.
DR EMBL; AB046355; BAB40950.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15605.1; -; Genomic_DNA.
DR PIR; H70069; H70069.
DR RefSeq; NP_391469.1; NC_000964.3.
DR RefSeq; WP_003243306.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P96738; -.
DR STRING; 224308.BSU35880; -.
DR PaxDb; P96738; -.
DR PRIDE; P96738; -.
DR DNASU; 936830; -.
DR EnsemblBacteria; CAB15605; CAB15605; BSU_35880.
DR GeneID; 936830; -.
DR KEGG; bsu:BSU35880; -.
DR PATRIC; fig|224308.179.peg.3884; -.
DR eggNOG; COG2843; Bacteria.
DR InParanoid; P96738; -.
DR OMA; HWGQEYD; -.
DR PhylomeDB; P96738; -.
DR BioCyc; BSUB:BSU35880-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR CDD; cd07381; MPP_CapA; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR019079; Capsule_synth_CapA.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF09587; PGA_cap; 1.
DR SMART; SM00854; PGA_cap; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 3: Inferred from homology;
KW Reference proteome.
FT CHAIN 1..380
FT /note="PGA biosynthesis protein CapA"
FT /id="PRO_0000089308"
FT VARIANT 164..165
FT /note="KQ -> TK (in strain: IFO 16449)"
SQ SEQUENCE 380 AA; 42760 MW; 3D173A556A674422 CRC64;
MKKELSFHEK LLKLTKQQKK KTNKHVFIAI PIVFVLMFAF MWAGKAETPK VKTYSDDVLS
ASFVGDIMMG RYVEKVTEQK GADSIFQYVE PIFRASDYVA GNFENPVTYQ KNYKQADKEI
HLQTNKESVK VLKDMNFTVL NSANNHAMDY GVQGMKDTLG EFAKQNLDIV GAGYSLSDAK
KKISYQKVNG VTIATLGFTD VSGKGFAAKK NTPGVLPADP EIFIPMISEA KKHADIVVVQ
SHWGQEYDND PNDRQRQLAR AMSDAGADII VGHHPHVLEP IEVYNGTVIF YSLGNFVFDQ
GWTRTRDSAL VQYHLKKNGT GRFEVTPIDI HEATPAPVKK DSLKQKTIIR ELTKDSNFAW
KVEDGKLTFD IDHSDKLKSK
